Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> Strains
The objectives of this study were to obtain and characterise polyphenolic extracts from red grape pomace of <i>Vitis vinifera</i> L. cv Graciano via conventional solvent extraction (SE) and green supercritical fluid extraction (SFE) and to evaluate their <i>in vitro</i> antib...
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2024-12-01
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| author | Rocío Fernández-Pérez Silvia Ayuso Cristina Moreta María-José Saiz-Abajo Miguel Gastón-Lorente Fernanda Ruiz-Larrea Carmen Tenorio |
| author_facet | Rocío Fernández-Pérez Silvia Ayuso Cristina Moreta María-José Saiz-Abajo Miguel Gastón-Lorente Fernanda Ruiz-Larrea Carmen Tenorio |
| author_sort | Rocío Fernández-Pérez |
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| description | The objectives of this study were to obtain and characterise polyphenolic extracts from red grape pomace of <i>Vitis vinifera</i> L. cv Graciano via conventional solvent extraction (SE) and green supercritical fluid extraction (SFE) and to evaluate their <i>in vitro</i> antibacterial activity against susceptible and multidrug-resistant <i>Escherichia coli</i> strains of intestinal origin. The SE and SFE methods were optimised, and ultra-performance liquid chromatography/mass spectrometry (UPLC/QqQ-MS/MS) analysis revealed 38 phenolic compounds in the SE sample, with anthocyanins being the predominant polyphenols, and 21 phenolic compounds in the SFE samples, among which hydroxybenzoic acids and flavonols were the predominant compounds. The SE and SFE samples showed antibacterial activity against both antibiotic-susceptible and -resistant <i>E. coli</i> strains, and minimal inhibitory concentration values were in the range of 1–4 mg/mL. The activity was bacteriostatic in all cases, and it was shown that a higher content of total polyphenols correlated with a higher antibacterial activity of the extracts. This study shows that red grape pomace of <i>Vitis vinifera</i> L. cv. Graciano is a rich source of bioactive phenolic compounds that can become an important feedstock for additives and other upgraded products of biotechnological interest, which can help to modulate intestinal microbiota and combat bacterial antibiotic resistance. |
| format | Article |
| id | doaj-art-3909d58d84154dcab87fd374b9c4112a |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-3909d58d84154dcab87fd374b9c4112a2025-01-10T13:17:31ZengMDPI AGFoods2304-81582024-12-011411710.3390/foods14010017Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> StrainsRocío Fernández-Pérez0Silvia Ayuso1Cristina Moreta2María-José Saiz-Abajo3Miguel Gastón-Lorente4Fernanda Ruiz-Larrea5Carmen Tenorio6Instituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja, Consejo Superior de Investigaciones Científicas (CSIC), Gobierno de La Rioja), 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja, Consejo Superior de Investigaciones Científicas (CSIC), Gobierno de La Rioja), 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja, Consejo Superior de Investigaciones Científicas (CSIC), Gobierno de La Rioja), 26007 Logroño, SpainNational Centre for Food Technology and Safety (CNTA), 31570 San Adrian, SpainNational Centre for Food Technology and Safety (CNTA), 31570 San Adrian, SpainInstituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja, Consejo Superior de Investigaciones Científicas (CSIC), Gobierno de La Rioja), 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja, Consejo Superior de Investigaciones Científicas (CSIC), Gobierno de La Rioja), 26007 Logroño, SpainThe objectives of this study were to obtain and characterise polyphenolic extracts from red grape pomace of <i>Vitis vinifera</i> L. cv Graciano via conventional solvent extraction (SE) and green supercritical fluid extraction (SFE) and to evaluate their <i>in vitro</i> antibacterial activity against susceptible and multidrug-resistant <i>Escherichia coli</i> strains of intestinal origin. The SE and SFE methods were optimised, and ultra-performance liquid chromatography/mass spectrometry (UPLC/QqQ-MS/MS) analysis revealed 38 phenolic compounds in the SE sample, with anthocyanins being the predominant polyphenols, and 21 phenolic compounds in the SFE samples, among which hydroxybenzoic acids and flavonols were the predominant compounds. The SE and SFE samples showed antibacterial activity against both antibiotic-susceptible and -resistant <i>E. coli</i> strains, and minimal inhibitory concentration values were in the range of 1–4 mg/mL. The activity was bacteriostatic in all cases, and it was shown that a higher content of total polyphenols correlated with a higher antibacterial activity of the extracts. This study shows that red grape pomace of <i>Vitis vinifera</i> L. cv. Graciano is a rich source of bioactive phenolic compounds that can become an important feedstock for additives and other upgraded products of biotechnological interest, which can help to modulate intestinal microbiota and combat bacterial antibiotic resistance.https://www.mdpi.com/2304-8158/14/1/17grape pomace<i>Vitis vinifera</i>polyphenolssupercritical fluid extraction<i>Escherichia coli</i>antibiotic resistance |
| spellingShingle | Rocío Fernández-Pérez Silvia Ayuso Cristina Moreta María-José Saiz-Abajo Miguel Gastón-Lorente Fernanda Ruiz-Larrea Carmen Tenorio Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> Strains Foods grape pomace <i>Vitis vinifera</i> polyphenols supercritical fluid extraction <i>Escherichia coli</i> antibiotic resistance |
| title | Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> Strains |
| title_full | Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> Strains |
| title_fullStr | Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> Strains |
| title_full_unstemmed | Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> Strains |
| title_short | Chemical Profile and Antibacterial Activity of <i>Vitis vinifera</i> L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO<sub>2</sub> Extraction Method Against Multidrug-Resistant <i>Escherichia coli</i> Strains |
| title_sort | chemical profile and antibacterial activity of i vitis vinifera i l cv graciano pomace extracts obtained by green supercritical co sub 2 sub extraction method against multidrug resistant i escherichia coli i strains |
| topic | grape pomace <i>Vitis vinifera</i> polyphenols supercritical fluid extraction <i>Escherichia coli</i> antibiotic resistance |
| url | https://www.mdpi.com/2304-8158/14/1/17 |
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