Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study

Abstract Background: Follicle-stimulating hormone receptor (FSHR) and luteinizing hormone/choriogonadotropin receptor (LHCGR) are integral to ovarian function, facilitating follicle development and maturation through their respective hormonal interactions. The influence of receptor polymorphisms on...

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Main Authors: Jayesh Amin, Naga Sandhya Alle, Ami Patel, Bansi Prajapathi, Paresh Makwana, Jaya Prakash, Kota Murali Krishna
Format: Article
Language:English
Published: Shahid Sadoughi University of Medical Sciences 2024-09-01
Series:International Journal of Reproductive BioMedicine
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Online Access:https://doi.org/10.18502/ijrm.v22i7.16962
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author Jayesh Amin
Naga Sandhya Alle
Ami Patel
Bansi Prajapathi
Paresh Makwana
Jaya Prakash
Kota Murali Krishna
author_facet Jayesh Amin
Naga Sandhya Alle
Ami Patel
Bansi Prajapathi
Paresh Makwana
Jaya Prakash
Kota Murali Krishna
author_sort Jayesh Amin
collection DOAJ
description Abstract Background: Follicle-stimulating hormone receptor (FSHR) and luteinizing hormone/choriogonadotropin receptor (LHCGR) are integral to ovarian function, facilitating follicle development and maturation through their respective hormonal interactions. The influence of receptor polymorphisms on the outcomes of freeze-all cycles remains unclear. Objective: This study investigates the impact of FSHR N680S and LHCGR N312S polymorphisms on clinical outcomes in freeze-all cycles. Materials and Methods: Women undergoing controlled ovarian stimulation for assisted reproductive technology participated in this study. They were administered a gonadotropin-releasing hormone antagonist protocol, with recombinant follicle-stimulating hormone (rFSH) dosages adjusted according to age, body mass index, antral follicle count, and individual hormonal responses. Additionally, human menopausal gonadotropin dosages were tailored based on the LHCGR N312S genetic variant. Results: Analysis revealed no significant differences in age, body mass index, antral follicle count, or marital status across the genotypes of FSHR N680S and LHCGR N312S. However, notable differences were observed in the rFSH dosage required daily and in total among the FSHR polymorphism genotypes. Genotypes of the LHCGR polymorphism correlated with fewer stimulation days. A significant interaction was observed between the 2 polymorphisms concerning total rFSH dosage. Conclusion: The presence of serine in the FSHR polymorphism was associated with higher rFSH dosage requirements. Both FSHR N680S and LHCGR N312S polymorphisms significantly influenced clinical pregnancy and live birth outcomes in freeze-all cycles, underscoring the potential of a pharmacogenomic approach to optimize hormone supplementation in controlled ovarian stimulation protocols during assisted reproductive technology treatments.
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publisher Shahid Sadoughi University of Medical Sciences
record_format Article
series International Journal of Reproductive BioMedicine
spelling doaj-art-3904372f12074d62a63bb722ccf608e32025-08-20T01:54:26ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722024-09-0122753955210.18502/ijrm.v22i7.16962ijrm.v22i7.16962Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control studyJayesh Amin0Naga Sandhya Alle1Ami Patel2Bansi Prajapathi3Paresh Makwana4Jaya Prakash5Kota Murali Krishna6 Wings IVF Women's Hospital, Ahmedabad, Gujarat, India. Wings IVF Women's Hospital, Ahmedabad, Gujarat, India. Wings IVF Women's Hospital, Ahmedabad, Gujarat, India. Wings IVF Women's Hospital, Ahmedabad, Gujarat, India. Wings IVF Women's Hospital, Ahmedabad, Gujarat, India. Life Fertility and Research Center, Collector Office Jn, Maharanipeta, Visakhapatnam, Andhra Pradesh, India. Life Fertility and Research Center, Collector Office Jn, Maharanipeta, Visakhapatnam, Andhra Pradesh, India.Abstract Background: Follicle-stimulating hormone receptor (FSHR) and luteinizing hormone/choriogonadotropin receptor (LHCGR) are integral to ovarian function, facilitating follicle development and maturation through their respective hormonal interactions. The influence of receptor polymorphisms on the outcomes of freeze-all cycles remains unclear. Objective: This study investigates the impact of FSHR N680S and LHCGR N312S polymorphisms on clinical outcomes in freeze-all cycles. Materials and Methods: Women undergoing controlled ovarian stimulation for assisted reproductive technology participated in this study. They were administered a gonadotropin-releasing hormone antagonist protocol, with recombinant follicle-stimulating hormone (rFSH) dosages adjusted according to age, body mass index, antral follicle count, and individual hormonal responses. Additionally, human menopausal gonadotropin dosages were tailored based on the LHCGR N312S genetic variant. Results: Analysis revealed no significant differences in age, body mass index, antral follicle count, or marital status across the genotypes of FSHR N680S and LHCGR N312S. However, notable differences were observed in the rFSH dosage required daily and in total among the FSHR polymorphism genotypes. Genotypes of the LHCGR polymorphism correlated with fewer stimulation days. A significant interaction was observed between the 2 polymorphisms concerning total rFSH dosage. Conclusion: The presence of serine in the FSHR polymorphism was associated with higher rFSH dosage requirements. Both FSHR N680S and LHCGR N312S polymorphisms significantly influenced clinical pregnancy and live birth outcomes in freeze-all cycles, underscoring the potential of a pharmacogenomic approach to optimize hormone supplementation in controlled ovarian stimulation protocols during assisted reproductive technology treatments.https://doi.org/10.18502/ijrm.v22i7.16962lhcgr, fshr, polymorphism.
spellingShingle Jayesh Amin
Naga Sandhya Alle
Ami Patel
Bansi Prajapathi
Paresh Makwana
Jaya Prakash
Kota Murali Krishna
Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study
International Journal of Reproductive BioMedicine
lhcgr, fshr, polymorphism.
title Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study
title_full Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study
title_fullStr Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study
title_full_unstemmed Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study
title_short Significance of FSHR and LHCGR gene polymorphisms on clinical outcomes in gonadotropin-releasing hormone antagonist protocol with freeze-all strategy: A case-control study
title_sort significance of fshr and lhcgr gene polymorphisms on clinical outcomes in gonadotropin releasing hormone antagonist protocol with freeze all strategy a case control study
topic lhcgr, fshr, polymorphism.
url https://doi.org/10.18502/ijrm.v22i7.16962
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