Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGE
Background: it is known that intrauterine growth retardation (IUGR) represents a risk factor for the deterioration of renal function as it can adversely impact on the number of nephrons developed in the kidney during nephrogenesis. An interesting molecule is the Cystatin-C (cyst-C): it is considered...
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Elsevier
2022-11-01
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| Series: | Pediatrics and Neonatology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1875957222001541 |
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| author | Chiara Grasselli |
| author_facet | Chiara Grasselli |
| author_sort | Chiara Grasselli |
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| description | Background: it is known that intrauterine growth retardation (IUGR) represents a risk factor for the deterioration of renal function as it can adversely impact on the number of nephrons developed in the kidney during nephrogenesis. An interesting molecule is the Cystatin-C (cyst-C): it is considered to have the potency to detect both glomerular and proximal renal injury. Recently, using a quantitative EIA cyst-C detection kit, we found increased levels of cyst-C in the urine of neonates with IUGR. Since cyst-C molecules can be present in both monomer and/or polymer forms, the purpose of this study is to investigate in which forms this molecule is present in the urine of IUGR neonates by Immunoblot SDS-PAGE in order to verify if the presence or absence of a particular type of cyst-C conformation can give more information about the renal functioning. Methods: urine samples were collected from 64 neonates with IUGR, and 86 healthy controls defined as appropriate for gestational age (AGA). Urinary cyst-C was investigated by the Immunoblot SDS-PAGE. Results: in all urine samples, SDS-PAGE analysis showed a reactivity of the IgG anti cyst-C with a complex of about 70 kDa. The monomer form at 13 KDa appeared in 78% of IUGR neonates and in 12% of AGA neonates. Conclusions: this study revealed the presence of monomer cyst-C in the urine of IUGR neonates, and suggests an insufficient and/or non-compensatory reabsorption by tubular cells. Monomeric cyst-C can be considered an early biochemical marker to identify and to select IUGR neonates who need to be monitored for risk of renal injury. |
| format | Article |
| id | doaj-art-38f3108c8acf444aaad8feaa289a1256 |
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| issn | 1875-9572 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
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| series | Pediatrics and Neonatology |
| spelling | doaj-art-38f3108c8acf444aaad8feaa289a12562025-08-20T01:56:19ZengElsevierPediatrics and Neonatology1875-95722022-11-0163659960410.1016/j.pedneo.2022.04.012Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGEChiara Grasselli0Department of Surgical and Biomedical Sciences, University of Perugia, Perugia, ItalyBackground: it is known that intrauterine growth retardation (IUGR) represents a risk factor for the deterioration of renal function as it can adversely impact on the number of nephrons developed in the kidney during nephrogenesis. An interesting molecule is the Cystatin-C (cyst-C): it is considered to have the potency to detect both glomerular and proximal renal injury. Recently, using a quantitative EIA cyst-C detection kit, we found increased levels of cyst-C in the urine of neonates with IUGR. Since cyst-C molecules can be present in both monomer and/or polymer forms, the purpose of this study is to investigate in which forms this molecule is present in the urine of IUGR neonates by Immunoblot SDS-PAGE in order to verify if the presence or absence of a particular type of cyst-C conformation can give more information about the renal functioning. Methods: urine samples were collected from 64 neonates with IUGR, and 86 healthy controls defined as appropriate for gestational age (AGA). Urinary cyst-C was investigated by the Immunoblot SDS-PAGE. Results: in all urine samples, SDS-PAGE analysis showed a reactivity of the IgG anti cyst-C with a complex of about 70 kDa. The monomer form at 13 KDa appeared in 78% of IUGR neonates and in 12% of AGA neonates. Conclusions: this study revealed the presence of monomer cyst-C in the urine of IUGR neonates, and suggests an insufficient and/or non-compensatory reabsorption by tubular cells. Monomeric cyst-C can be considered an early biochemical marker to identify and to select IUGR neonates who need to be monitored for risk of renal injury.http://www.sciencedirect.com/science/article/pii/S1875957222001541biochemical markercyst-Cearly diagnosiskidney damageImmunoblot-SDS PAGE |
| spellingShingle | Chiara Grasselli Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGE Pediatrics and Neonatology biochemical marker cyst-C early diagnosis kidney damage Immunoblot-SDS PAGE |
| title | Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGE |
| title_full | Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGE |
| title_fullStr | Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGE |
| title_full_unstemmed | Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGE |
| title_short | Detection of urinary cystatin-c in IUGR neonates by immunoblot SDS-PAGE |
| title_sort | detection of urinary cystatin c in iugr neonates by immunoblot sds page |
| topic | biochemical marker cyst-C early diagnosis kidney damage Immunoblot-SDS PAGE |
| url | http://www.sciencedirect.com/science/article/pii/S1875957222001541 |
| work_keys_str_mv | AT chiaragrasselli detectionofurinarycystatinciniugrneonatesbyimmunoblotsdspage |