Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma
Summary: Here, we present a protocol for developing an in vitro 3D model of neuroblastoma through bioprinting, incorporating key components of the tumor microenvironment (TME), including vasculature and cancer cell line spheroids. We describe steps for the preparation, bioprinting, and evaluation of...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001315 |
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| author | Mehdi Salar Amoli Sarah Rezapourdamanab Linqi Jin Melissa A. Cadena Kaveeta Kaw Vani Sridhar Mostafa Meselhe Sarah Krikor Morteza Mahmoudi Liqun Ning Jenny Shim Martin L. Tomov Kelly C. Goldsmith Vahid Serpooshan |
| author_facet | Mehdi Salar Amoli Sarah Rezapourdamanab Linqi Jin Melissa A. Cadena Kaveeta Kaw Vani Sridhar Mostafa Meselhe Sarah Krikor Morteza Mahmoudi Liqun Ning Jenny Shim Martin L. Tomov Kelly C. Goldsmith Vahid Serpooshan |
| author_sort | Mehdi Salar Amoli |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for developing an in vitro 3D model of neuroblastoma through bioprinting, incorporating key components of the tumor microenvironment (TME), including vasculature and cancer cell line spheroids. We describe steps for the preparation, bioprinting, and evaluation of tumor models and their use to assess the impact of TME on solid tumor behavior. We report procedures for bioink preparation, bioprinting, the measurement of mechanical properties and fidelity, as well as the viability of different cell types and their interactions.For complete details on the use and execution of this protocol, please refer to Ning et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-38eed314e4ab464683376554a7746f42 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-38eed314e4ab464683376554a7746f422025-08-20T02:16:29ZengElsevierSTAR Protocols2666-16672025-06-016210372510.1016/j.xpro.2025.103725Protocol for 3D bioprinting of a 3D in vitro model of neuroblastomaMehdi Salar Amoli0Sarah Rezapourdamanab1Linqi Jin2Melissa A. Cadena3Kaveeta Kaw4Vani Sridhar5Mostafa Meselhe6Sarah Krikor7Morteza Mahmoudi8Liqun Ning9Jenny Shim10Martin L. Tomov11Kelly C. Goldsmith12Vahid Serpooshan13Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Corresponding authorDepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USAPrecision Health Program, Michigan State University, East Lansing, MI, USAPrecision Health Program, Michigan State University, East Lansing, MI, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Mechanical Engineering, Cleveland State University, Cleveland, OH 44115, USAWinship Cancer Institute, Emory University, Atlanta, GA, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Aflac Cancer and Blood Disorders Center, The Children’s Healthcare of Atlanta, Atlanta, GA, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USAWinship Cancer Institute, Emory University, Atlanta, GA, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Aflac Cancer and Blood Disorders Center, The Children’s Healthcare of Atlanta, Atlanta, GA, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA; Corresponding authorSummary: Here, we present a protocol for developing an in vitro 3D model of neuroblastoma through bioprinting, incorporating key components of the tumor microenvironment (TME), including vasculature and cancer cell line spheroids. We describe steps for the preparation, bioprinting, and evaluation of tumor models and their use to assess the impact of TME on solid tumor behavior. We report procedures for bioink preparation, bioprinting, the measurement of mechanical properties and fidelity, as well as the viability of different cell types and their interactions.For complete details on the use and execution of this protocol, please refer to Ning et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001315cell culturecancerbiotechnology and bioengineering |
| spellingShingle | Mehdi Salar Amoli Sarah Rezapourdamanab Linqi Jin Melissa A. Cadena Kaveeta Kaw Vani Sridhar Mostafa Meselhe Sarah Krikor Morteza Mahmoudi Liqun Ning Jenny Shim Martin L. Tomov Kelly C. Goldsmith Vahid Serpooshan Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma STAR Protocols cell culture cancer biotechnology and bioengineering |
| title | Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma |
| title_full | Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma |
| title_fullStr | Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma |
| title_full_unstemmed | Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma |
| title_short | Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma |
| title_sort | protocol for 3d bioprinting of a 3d in vitro model of neuroblastoma |
| topic | cell culture cancer biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725001315 |
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