Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells
Aim. CD133+ stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended to develop a nonviral system for appropriate cell engineering. Materials and Methods. Mod...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7152761 |
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| _version_ | 1850105708666683392 |
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| author | Paula Müller Natalia Voronina Frauke Hausburg Cornelia A. Lux Frank Wiekhorst Gustav Steinhoff Robert David |
| author_facet | Paula Müller Natalia Voronina Frauke Hausburg Cornelia A. Lux Frank Wiekhorst Gustav Steinhoff Robert David |
| author_sort | Paula Müller |
| collection | DOAJ |
| description | Aim. CD133+ stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended to develop a nonviral system for appropriate cell engineering. Materials and Methods. Modification of human CD133+ stem cells with magnetic polyplexes carrying microRNA was studied in terms of efficiency, safety, and targeting potential. Results. High microRNA uptake rates (~80–90%) were achieved without affecting CD133+ stem cell properties. Modified cells can be magnetically guided. Conclusion. We developed a safe and efficient protocol for CD133+ stem cell modification. Our work may become a basis to improve stem cell therapeutical effects as well as their monitoring with magnetic resonance imaging. |
| format | Article |
| id | doaj-art-38e5c8c31d114fa3b99a4b080b171acc |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-38e5c8c31d114fa3b99a4b080b171acc2025-08-20T02:38:59ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/71527617152761Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem CellsPaula Müller0Natalia Voronina1Frauke Hausburg2Cornelia A. Lux3Frank Wiekhorst4Gustav Steinhoff5Robert David6Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyReference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyReference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyReference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyPhysikalisch-Technische Bundesanstalt, Abbestraße 2-12, Berlin 10587, GermanyReference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyReference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyAim. CD133+ stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended to develop a nonviral system for appropriate cell engineering. Materials and Methods. Modification of human CD133+ stem cells with magnetic polyplexes carrying microRNA was studied in terms of efficiency, safety, and targeting potential. Results. High microRNA uptake rates (~80–90%) were achieved without affecting CD133+ stem cell properties. Modified cells can be magnetically guided. Conclusion. We developed a safe and efficient protocol for CD133+ stem cell modification. Our work may become a basis to improve stem cell therapeutical effects as well as their monitoring with magnetic resonance imaging.http://dx.doi.org/10.1155/2016/7152761 |
| spellingShingle | Paula Müller Natalia Voronina Frauke Hausburg Cornelia A. Lux Frank Wiekhorst Gustav Steinhoff Robert David Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells Stem Cells International |
| title | Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells |
| title_full | Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells |
| title_fullStr | Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells |
| title_full_unstemmed | Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells |
| title_short | Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells |
| title_sort | magnet bead based microrna delivery system to modify cd133 stem cells |
| url | http://dx.doi.org/10.1155/2016/7152761 |
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