Comparison of Commercially Available Thermostable DNA Polymerases with Reverse Transcriptase Activity in Coupled Reverse Transcription Polymerase Chain Reaction Assays

Comparison of Commercially Available Thermostable DNA Polymerases with Reverse Transcriptase Activity in Coupled Reverse Transcription Polymerase Chain Reaction Assays

Reverse transcription polymerase chain reaction (RT-PCR) is an important tool for the detection of target RNA molecules and the assay of RNA pathogens. Coupled RT-PCR is performed with an enzyme mixture containing a reverse transcriptase and a thermostable DNA polymerase. To date, several biotechnol...

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Bibliographic Details
Main Authors: Evgeniya V. Smirnova, Konstantin A. Blagodatskikh, Ekaterina V. Barsova, Dmitriy A. Varlamov, Vladimir M. Kramarov, Konstantin B. Ignatov
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Methods and Protocols
Subjects:
reverse transcriptase
thermostable DNA polymerase
reverse transcription polymerase chain reaction
SARS-CoV2
beta-2-microglobulin mRNA
<i>GAPDH</i> mRNA
Online Access:https://www.mdpi.com/2409-9279/8/1/11
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