Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorial
Comprehensive two-dimensional liquid chromatography (LC×LC) is a valuable tool for the analysis of very complex samples. LC×LC, which involves the coupling of two or more orthogonal separation systems, is a potential alternative to the conventional liquid-chromatography (1D-LC) approach. The profits...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
|
| Series: | Journal of Chromatography Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772391725000209 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849321305667534848 |
|---|---|
| author | Katia Arena Francesco Cacciola Roberto Laganà Vinci Paola Dugo Luigi Mondello |
| author_facet | Katia Arena Francesco Cacciola Roberto Laganà Vinci Paola Dugo Luigi Mondello |
| author_sort | Katia Arena |
| collection | DOAJ |
| description | Comprehensive two-dimensional liquid chromatography (LC×LC) is a valuable tool for the analysis of very complex samples. LC×LC, which involves the coupling of two or more orthogonal separation systems, is a potential alternative to the conventional liquid-chromatography (1D-LC) approach. The profits of such a technique, compared to the 1D-LC approach, are the increased resolving power along with significant reduced ion enhancement or suppression phenomena in the MS source. This tutorial aims to provide detailed information on theoretical and practical aspects of the LC×LC approach with a particular focus on column combination, method optimization and separation performance. In addition, future perspectives and new trends are highlighted. |
| format | Article |
| id | doaj-art-38d76cd94ea74ce3be0c3f5008a46737 |
| institution | Kabale University |
| issn | 2772-3917 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Chromatography Open |
| spelling | doaj-art-38d76cd94ea74ce3be0c3f5008a467372025-08-20T03:49:46ZengElsevierJournal of Chromatography Open2772-39172025-05-01710022210.1016/j.jcoa.2025.100222Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorialKatia Arena0Francesco Cacciola1Roberto Laganà Vinci2Paola Dugo3Luigi Mondello4Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, 98168 Messina, ItalyMessina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, 98168 Messina, Italy; Corresponding author.Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, 98168 Messina, ItalyMessina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, 98168 Messina, Italy; Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, 98168 Messina, ItalyMessina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, 98168 Messina, Italy; Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, 98168 Messina, ItalyComprehensive two-dimensional liquid chromatography (LC×LC) is a valuable tool for the analysis of very complex samples. LC×LC, which involves the coupling of two or more orthogonal separation systems, is a potential alternative to the conventional liquid-chromatography (1D-LC) approach. The profits of such a technique, compared to the 1D-LC approach, are the increased resolving power along with significant reduced ion enhancement or suppression phenomena in the MS source. This tutorial aims to provide detailed information on theoretical and practical aspects of the LC×LC approach with a particular focus on column combination, method optimization and separation performance. In addition, future perspectives and new trends are highlighted.http://www.sciencedirect.com/science/article/pii/S2772391725000209LC×LCMultidimensional chromatographyComplex samples, method optimization |
| spellingShingle | Katia Arena Francesco Cacciola Roberto Laganà Vinci Paola Dugo Luigi Mondello Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorial Journal of Chromatography Open LC×LC Multidimensional chromatography Complex samples, method optimization |
| title | Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorial |
| title_full | Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorial |
| title_fullStr | Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorial |
| title_full_unstemmed | Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorial |
| title_short | Exploration of comprehensive two-dimensional liquid chromatography: Theoretical aspects and practical considerations - A tutorial |
| title_sort | exploration of comprehensive two dimensional liquid chromatography theoretical aspects and practical considerations a tutorial |
| topic | LC×LC Multidimensional chromatography Complex samples, method optimization |
| url | http://www.sciencedirect.com/science/article/pii/S2772391725000209 |
| work_keys_str_mv | AT katiaarena explorationofcomprehensivetwodimensionalliquidchromatographytheoreticalaspectsandpracticalconsiderationsatutorial AT francescocacciola explorationofcomprehensivetwodimensionalliquidchromatographytheoreticalaspectsandpracticalconsiderationsatutorial AT robertolaganavinci explorationofcomprehensivetwodimensionalliquidchromatographytheoreticalaspectsandpracticalconsiderationsatutorial AT paoladugo explorationofcomprehensivetwodimensionalliquidchromatographytheoreticalaspectsandpracticalconsiderationsatutorial AT luigimondello explorationofcomprehensivetwodimensionalliquidchromatographytheoreticalaspectsandpracticalconsiderationsatutorial |