Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method

Mexico is home to a rich variety of fermented beverages made from both wild and domesticated plant species. Fermentation practices vary, with producers using either wild or inoculated techniques to obtain culturally valued final products. It is generally assumed that wild fermentations yield a great...

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Main Authors: César Ojeda-Linares, Alejandro Casas, Patricia Severiano-Pérez, Marcela Sandoval-Velasco, Yolanda M. García-Rodríguez, Francisco J. Espinosa-García
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Fermentation
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Online Access:https://www.mdpi.com/2311-5637/11/5/275
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author César Ojeda-Linares
Alejandro Casas
Patricia Severiano-Pérez
Marcela Sandoval-Velasco
Yolanda M. García-Rodríguez
Francisco J. Espinosa-García
author_facet César Ojeda-Linares
Alejandro Casas
Patricia Severiano-Pérez
Marcela Sandoval-Velasco
Yolanda M. García-Rodríguez
Francisco J. Espinosa-García
author_sort César Ojeda-Linares
collection DOAJ
description Mexico is home to a rich variety of fermented beverages made from both wild and domesticated plant species. Fermentation practices vary, with producers using either wild or inoculated techniques to obtain culturally valued final products. It is generally assumed that wild fermentations yield a greater diversity of volatile compounds compared to inoculated fermentations, as the latter tend to reduce microbial diversity throughout the process. However, this pattern remains largely unexplored in relation to the volatile profiles of traditionally fermented cactus-based beverages. Despite this assumption, comparative studies examining these profiles across different fermentation methods are scarce, especially given that these beverages are not produced under standardized conditions. To investigate this, we used GC-MS to characterize the aroma profile of <i>colonche</i>, a traditional fermented beverage made primarily from <i>Opuntia streptacantha</i> fruits. <i>Colonche</i> is produced by both wild and inoculated fermentation methods. In addition, a rapid sensory evaluation using the modified Flash Profile (mFP) technique was performed to evaluate flavor differences between the fermentation methods. A total of 55 volatile compounds were identified, with wild fermentations showing greater diversity (55) than inoculated fermentations (50). Most compounds overlapped, but five were unique to spontaneous fermentations, contributing to distinct sensory profiles. The mFP results also indicate that sensory attributes vary by fermentation type, with wild fermentations being more strongly associated with positive descriptors such as taste and smell, while inoculated samples have a distinctly pungent aftertaste. These findings highlight <i>colonche</i> not only as a reservoir of microbial diversity in arid regions but also as a culturally significant beverage with complex sensory attributes. Recognizing and preserving these attributes is essential for safeguarding traditional foodscapes.
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publishDate 2025-05-01
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series Fermentation
spelling doaj-art-38d5e9e109b94d1f995cce261af6b0f02025-08-20T02:33:48ZengMDPI AGFermentation2311-56372025-05-0111527510.3390/fermentation11050275Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation MethodCésar Ojeda-Linares0Alejandro Casas1Patricia Severiano-Pérez2Marcela Sandoval-Velasco3Yolanda M. García-Rodríguez4Francisco J. Espinosa-García5Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62210, MexicoInstituto de Investigaciones en Ecosistemas y Sostenibilidad, Universidad Nacional Autónoma de México, Morelia 58190, MexicoFacultad de Química, Departamento de Alimentos y Biotecnología, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoCentro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62210, MexicoInstituto de Investigaciones en Ecosistemas y Sostenibilidad, Universidad Nacional Autónoma de México, Morelia 58190, MexicoInstituto de Investigaciones en Ecosistemas y Sostenibilidad, Universidad Nacional Autónoma de México, Morelia 58190, MexicoMexico is home to a rich variety of fermented beverages made from both wild and domesticated plant species. Fermentation practices vary, with producers using either wild or inoculated techniques to obtain culturally valued final products. It is generally assumed that wild fermentations yield a greater diversity of volatile compounds compared to inoculated fermentations, as the latter tend to reduce microbial diversity throughout the process. However, this pattern remains largely unexplored in relation to the volatile profiles of traditionally fermented cactus-based beverages. Despite this assumption, comparative studies examining these profiles across different fermentation methods are scarce, especially given that these beverages are not produced under standardized conditions. To investigate this, we used GC-MS to characterize the aroma profile of <i>colonche</i>, a traditional fermented beverage made primarily from <i>Opuntia streptacantha</i> fruits. <i>Colonche</i> is produced by both wild and inoculated fermentation methods. In addition, a rapid sensory evaluation using the modified Flash Profile (mFP) technique was performed to evaluate flavor differences between the fermentation methods. A total of 55 volatile compounds were identified, with wild fermentations showing greater diversity (55) than inoculated fermentations (50). Most compounds overlapped, but five were unique to spontaneous fermentations, contributing to distinct sensory profiles. The mFP results also indicate that sensory attributes vary by fermentation type, with wild fermentations being more strongly associated with positive descriptors such as taste and smell, while inoculated samples have a distinctly pungent aftertaste. These findings highlight <i>colonche</i> not only as a reservoir of microbial diversity in arid regions but also as a culturally significant beverage with complex sensory attributes. Recognizing and preserving these attributes is essential for safeguarding traditional foodscapes.https://www.mdpi.com/2311-5637/11/5/275ethnomicrobiologyvolatile compoundssensory evaluationmodified flash profileGC–MS
spellingShingle César Ojeda-Linares
Alejandro Casas
Patricia Severiano-Pérez
Marcela Sandoval-Velasco
Yolanda M. García-Rodríguez
Francisco J. Espinosa-García
Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method
Fermentation
ethnomicrobiology
volatile compounds
sensory evaluation
modified flash profile
GC–MS
title Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method
title_full Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method
title_fullStr Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method
title_full_unstemmed Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method
title_short Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method
title_sort exploring volatile profiles in cactus based fermented beverages effects of fermentation method
topic ethnomicrobiology
volatile compounds
sensory evaluation
modified flash profile
GC–MS
url https://www.mdpi.com/2311-5637/11/5/275
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