Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy
Abstract Mild-temperature photothermal therapy (MPTT) advances anticancer management by regulating reactive oxygen species (ROS) and lipid peroxides (LPO) to inhibit the overexpression of heat shock protein 70 (HSP70), thus decreasing the cellular heat resistance and increasing the efficacy of tumor...
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BMC
2025-05-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03379-x |
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| author | Binyue Zhang Yanchun Ma Qi Liu Shutong Wu Lin Chen Chunmei Jiang Haonan Chen Hongyan Jia Ziliang Zheng Ruiping Zhang |
| author_facet | Binyue Zhang Yanchun Ma Qi Liu Shutong Wu Lin Chen Chunmei Jiang Haonan Chen Hongyan Jia Ziliang Zheng Ruiping Zhang |
| author_sort | Binyue Zhang |
| collection | DOAJ |
| description | Abstract Mild-temperature photothermal therapy (MPTT) advances anticancer management by regulating reactive oxygen species (ROS) and lipid peroxides (LPO) to inhibit the overexpression of heat shock protein 70 (HSP70), thus decreasing the cellular heat resistance and increasing the efficacy of tumor ablation. However, formidable challenge remains on the traditional MPTT without imaging-guided optimal treatment time point, thus inadequate HSP70 blockage would potentially further diminish the effectiveness of MPTT. Herein, a novel biomimetic nanoprobe (Cu-ABTS@CCMs) is developed, based on encapsulating the multifunctional Cu nanoparticles and ROS-responsive 2,2′-azino-bis (3-ethylbenzothiazole-6- sulphonic acid) (ABTS) within cancer cell membranes (CCMs) to ensure second near-infrared photoacoustic (NIR-II PA) imaging-guided precise MPTT time point. The core Cu nanoparticles achieve highly effective HSP70 blockage via a nearly simultaneous cascade of photocatalytic O2-generation and dual ROS/LPO accumulation. Triggered by self-enhanced ROS/LPO up-regulation, the ABTS can correspondingly oxidize to ABTS•+, which further leads the real-time ratiometric PA signals (ABTS•+-PA730/Cu-PA960) that show highly accurate visualization of ROS and quantitatively convert into dynamic tracking of the changes in HSP70 blockage. The intelligent dual-modality imaging information will provide more possibilities for the optimal time-point and site-specificity of MPTT and potential avenues for the development of clinical breast cancer treatments. |
| format | Article |
| id | doaj-art-38bf62ccf92d4e2982985fac414b01e9 |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-38bf62ccf92d4e2982985fac414b01e92025-08-20T01:51:32ZengBMCJournal of Nanobiotechnology1477-31552025-05-0123111510.1186/s12951-025-03379-xVisualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategyBinyue Zhang0Yanchun Ma1Qi Liu2Shutong Wu3Lin Chen4Chunmei Jiang5Haonan Chen6Hongyan Jia7Ziliang Zheng8Ruiping Zhang9Laboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Translational Medicine Research Center, Department of Pathology, Shanxi Medical UniversityLaboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Laboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Laboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Laboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Laboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Department of Breast Surgery, First Hospital of Shanxi Medical UniversityLaboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Laboratory of Molecular Imaging, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital)Abstract Mild-temperature photothermal therapy (MPTT) advances anticancer management by regulating reactive oxygen species (ROS) and lipid peroxides (LPO) to inhibit the overexpression of heat shock protein 70 (HSP70), thus decreasing the cellular heat resistance and increasing the efficacy of tumor ablation. However, formidable challenge remains on the traditional MPTT without imaging-guided optimal treatment time point, thus inadequate HSP70 blockage would potentially further diminish the effectiveness of MPTT. Herein, a novel biomimetic nanoprobe (Cu-ABTS@CCMs) is developed, based on encapsulating the multifunctional Cu nanoparticles and ROS-responsive 2,2′-azino-bis (3-ethylbenzothiazole-6- sulphonic acid) (ABTS) within cancer cell membranes (CCMs) to ensure second near-infrared photoacoustic (NIR-II PA) imaging-guided precise MPTT time point. The core Cu nanoparticles achieve highly effective HSP70 blockage via a nearly simultaneous cascade of photocatalytic O2-generation and dual ROS/LPO accumulation. Triggered by self-enhanced ROS/LPO up-regulation, the ABTS can correspondingly oxidize to ABTS•+, which further leads the real-time ratiometric PA signals (ABTS•+-PA730/Cu-PA960) that show highly accurate visualization of ROS and quantitatively convert into dynamic tracking of the changes in HSP70 blockage. The intelligent dual-modality imaging information will provide more possibilities for the optimal time-point and site-specificity of MPTT and potential avenues for the development of clinical breast cancer treatments.https://doi.org/10.1186/s12951-025-03379-xDual-modal imagingRatiometric photoacoustic imagingSecond near-infrared windowMild-photothermal therapyBreast cancer |
| spellingShingle | Binyue Zhang Yanchun Ma Qi Liu Shutong Wu Lin Chen Chunmei Jiang Haonan Chen Hongyan Jia Ziliang Zheng Ruiping Zhang Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy Journal of Nanobiotechnology Dual-modal imaging Ratiometric photoacoustic imaging Second near-infrared window Mild-photothermal therapy Breast cancer |
| title | Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy |
| title_full | Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy |
| title_fullStr | Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy |
| title_full_unstemmed | Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy |
| title_short | Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy |
| title_sort | visualization of hsp70 regulated mild photothermal therapy for synergistic tumor treatment a precise space time mild temperature photothermal ablation strategy |
| topic | Dual-modal imaging Ratiometric photoacoustic imaging Second near-infrared window Mild-photothermal therapy Breast cancer |
| url | https://doi.org/10.1186/s12951-025-03379-x |
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