Discovery of novel mycoviruses from fungi associated with mango leaf spots
Mango (Mangifera indica) is a commercially significant fruit crop cultivated globally. However, leaf spot diseases are common in mango orchards, which severely impact the yield. Mycoviruses hold promise as potential biocontrol agents. To investigate this possibility, fungi were isolated from mango l...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1545534/full |
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| author | Qihua Wang Mengyi Chen Yanling Xie |
| author_facet | Qihua Wang Mengyi Chen Yanling Xie |
| author_sort | Qihua Wang |
| collection | DOAJ |
| description | Mango (Mangifera indica) is a commercially significant fruit crop cultivated globally. However, leaf spot diseases are common in mango orchards, which severely impact the yield. Mycoviruses hold promise as potential biocontrol agents. To investigate this possibility, fungi were isolated from mango leaf spot lesions, resulting in the identification of six strains that contained double-stranded RNA (dsRNA). Through BLASTx analysis of the NCBI non-redundant database, 27 mycovirus-related contigs were identified, which corresponded to 10 distinct viruses grouped into 8 lineages: Alternaviridae, Chrysoviridae, Partitiviridae, Polymycoviridae, Orthototiviridae, Deltaflexiviridae, Narnaviridae, and Bunyaviricetes. Full genomic sequences of these viruses were characterized and confirmed to be associated with their host fungi. The findings included six novel mycoviruses, three previously unreported viruses discovered in new hosts, and one virus strain. These results highlight the diversity and taxonomy of mycoviruses found in fungi associated with mango leaf spots. |
| format | Article |
| id | doaj-art-38b07c91654f46ceb533ce4ad58bb9e6 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-38b07c91654f46ceb533ce4ad58bb9e62025-08-20T03:04:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15455341545534Discovery of novel mycoviruses from fungi associated with mango leaf spotsQihua Wang0Mengyi Chen1Yanling Xie2Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang Key Laboratory of Tropical Crop Genetic Improvement, Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, ChinaKey Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang Key Laboratory of Tropical Crop Genetic Improvement, Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, ChinaMango (Mangifera indica) is a commercially significant fruit crop cultivated globally. However, leaf spot diseases are common in mango orchards, which severely impact the yield. Mycoviruses hold promise as potential biocontrol agents. To investigate this possibility, fungi were isolated from mango leaf spot lesions, resulting in the identification of six strains that contained double-stranded RNA (dsRNA). Through BLASTx analysis of the NCBI non-redundant database, 27 mycovirus-related contigs were identified, which corresponded to 10 distinct viruses grouped into 8 lineages: Alternaviridae, Chrysoviridae, Partitiviridae, Polymycoviridae, Orthototiviridae, Deltaflexiviridae, Narnaviridae, and Bunyaviricetes. Full genomic sequences of these viruses were characterized and confirmed to be associated with their host fungi. The findings included six novel mycoviruses, three previously unreported viruses discovered in new hosts, and one virus strain. These results highlight the diversity and taxonomy of mycoviruses found in fungi associated with mango leaf spots.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1545534/fullMangifera indicaleaf spotsnext-generation sequencingmycovirusviral diversityviral evolution |
| spellingShingle | Qihua Wang Mengyi Chen Yanling Xie Discovery of novel mycoviruses from fungi associated with mango leaf spots Frontiers in Microbiology Mangifera indica leaf spots next-generation sequencing mycovirus viral diversity viral evolution |
| title | Discovery of novel mycoviruses from fungi associated with mango leaf spots |
| title_full | Discovery of novel mycoviruses from fungi associated with mango leaf spots |
| title_fullStr | Discovery of novel mycoviruses from fungi associated with mango leaf spots |
| title_full_unstemmed | Discovery of novel mycoviruses from fungi associated with mango leaf spots |
| title_short | Discovery of novel mycoviruses from fungi associated with mango leaf spots |
| title_sort | discovery of novel mycoviruses from fungi associated with mango leaf spots |
| topic | Mangifera indica leaf spots next-generation sequencing mycovirus viral diversity viral evolution |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1545534/full |
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