Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study
Regulatory Foxp3-expressing T cells (Tregs), IL-10-producing B cells (Bregs), and IDO-expressing dendritic cells (DCregs) downregulate inflammatory processes and induces peripheral tolerance. These subpopulations also might participate in maintaining allograft immunological quiescence in kidney tran...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2012/256960 |
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| author | Janette Furuzawa-Carballeda Guadalupe Lima Perla Simancas Dolores Ramos-Bello Margaret Simancas Ian C. Bostock Mario Vilatobá Bernardo Gabilondo Julio Granados Luis Morales-Buenrostro Josefina Alberú Luis Llorente |
| author_facet | Janette Furuzawa-Carballeda Guadalupe Lima Perla Simancas Dolores Ramos-Bello Margaret Simancas Ian C. Bostock Mario Vilatobá Bernardo Gabilondo Julio Granados Luis Morales-Buenrostro Josefina Alberú Luis Llorente |
| author_sort | Janette Furuzawa-Carballeda |
| collection | DOAJ |
| description | Regulatory Foxp3-expressing T cells (Tregs), IL-10-producing B cells (Bregs), and IDO-expressing dendritic cells (DCregs) downregulate inflammatory processes and induces peripheral tolerance. These subpopulations also might participate in maintaining allograft immunological quiescence in kidney transplant recipients (KTRs) with an excellent long-term graft function under immunosuppression (ELTGF). The aim of the study was to characterize and to enumerate peripheral Tregs, Bregs, and DCregs in KTR with an ELTGF for more than 5 years after transplant. Fourteen KTR with an ELTGF, 9 KTR with chronic graft dysfunction (CGD), and 12 healthy donors (HDs) were included in the study. CD19+-expressing peripheral B lymphocytes were purified by positive selection. IL-10-producing B cells, CD4+/CD25hi, and CD8+/CD28− Tregs, as well as CCR6+/CD123+/IDO+ DCs, were quantitated by flow cytometry. IL-10-producing Bregs (immature/transitional, but not CD19+/CD38hi/CD24hi/CD27+B10 cells), CCR6+/CD123+/IDO+ DCs, and Tregs from ELTGF patients had similar or higher percentages versus HD (P<0.05). By contrast, number of Tregs, DCregs, and Bregs except for CD27+B10 cells from CGD patients had lower levels versus HD and ELTGF patients (P<0.05). The findings of this exploratory study might suggest that in ELTGF patients, peripheral tolerance mechanisms could be directly involved in the maintenance of a quiescent immunologic state and graft function stability. |
| format | Article |
| id | doaj-art-38a6d71a098c4ea2877251c58c76e5ea |
| institution | Kabale University |
| issn | 2090-0007 2090-0015 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Transplantation |
| spelling | doaj-art-38a6d71a098c4ea2877251c58c76e5ea2025-02-03T01:21:47ZengWileyJournal of Transplantation2090-00072090-00152012-01-01201210.1155/2012/256960256960Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional StudyJanette Furuzawa-Carballeda0Guadalupe Lima1Perla Simancas2Dolores Ramos-Bello3Margaret Simancas4Ian C. Bostock5Mario Vilatobá6Bernardo Gabilondo7Julio Granados8Luis Morales-Buenrostro9Josefina Alberú10Luis Llorente11Department of Immunology and Rheumatology, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Immunology and Rheumatology, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Transplantation, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Immunology and Rheumatology, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Transplantation, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Transplantation, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Transplantation, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Transplantation, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Transplantation, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Nephrology, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Transplantation, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoDepartment of Immunology and Rheumatology, National Institute of Medical Sciences and Nutrition, 14000 Mexico City, DF, MexicoRegulatory Foxp3-expressing T cells (Tregs), IL-10-producing B cells (Bregs), and IDO-expressing dendritic cells (DCregs) downregulate inflammatory processes and induces peripheral tolerance. These subpopulations also might participate in maintaining allograft immunological quiescence in kidney transplant recipients (KTRs) with an excellent long-term graft function under immunosuppression (ELTGF). The aim of the study was to characterize and to enumerate peripheral Tregs, Bregs, and DCregs in KTR with an ELTGF for more than 5 years after transplant. Fourteen KTR with an ELTGF, 9 KTR with chronic graft dysfunction (CGD), and 12 healthy donors (HDs) were included in the study. CD19+-expressing peripheral B lymphocytes were purified by positive selection. IL-10-producing B cells, CD4+/CD25hi, and CD8+/CD28− Tregs, as well as CCR6+/CD123+/IDO+ DCs, were quantitated by flow cytometry. IL-10-producing Bregs (immature/transitional, but not CD19+/CD38hi/CD24hi/CD27+B10 cells), CCR6+/CD123+/IDO+ DCs, and Tregs from ELTGF patients had similar or higher percentages versus HD (P<0.05). By contrast, number of Tregs, DCregs, and Bregs except for CD27+B10 cells from CGD patients had lower levels versus HD and ELTGF patients (P<0.05). The findings of this exploratory study might suggest that in ELTGF patients, peripheral tolerance mechanisms could be directly involved in the maintenance of a quiescent immunologic state and graft function stability.http://dx.doi.org/10.1155/2012/256960 |
| spellingShingle | Janette Furuzawa-Carballeda Guadalupe Lima Perla Simancas Dolores Ramos-Bello Margaret Simancas Ian C. Bostock Mario Vilatobá Bernardo Gabilondo Julio Granados Luis Morales-Buenrostro Josefina Alberú Luis Llorente Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study Journal of Transplantation |
| title | Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study |
| title_full | Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study |
| title_fullStr | Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study |
| title_full_unstemmed | Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study |
| title_short | Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study |
| title_sort | peripheral regulatory cells immunophenotyping in kidney transplant recipients with different clinical profiles a cross sectional study |
| url | http://dx.doi.org/10.1155/2012/256960 |
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