Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity
Lipopolysaccharide- (LPS-) mediated systemic inflammation plays a critical role in neurodegenerative diseases. The present study was conducted to evaluate the protective effects of epigallocatechin gallate (EGCG), the major component in green tea, on LPS-mediated inflammation and neurotoxicity. LPS...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4962351 |
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| author | Jin-Biao Liu Li Zhou Yi-Zhong Wang Xu Wang Yu Zhou Wen-Zhe Ho Jie-Liang Li |
| author_facet | Jin-Biao Liu Li Zhou Yi-Zhong Wang Xu Wang Yu Zhou Wen-Zhe Ho Jie-Liang Li |
| author_sort | Jin-Biao Liu |
| collection | DOAJ |
| description | Lipopolysaccharide- (LPS-) mediated systemic inflammation plays a critical role in neurodegenerative diseases. The present study was conducted to evaluate the protective effects of epigallocatechin gallate (EGCG), the major component in green tea, on LPS-mediated inflammation and neurotoxicity. LPS treatment of macrophages induced expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6). However, EGCG pretreatment of macrophages significantly inhibited LPS-mediated induction of these cytokines. In addition, EGCG significantly diminished LPS-induced inflammatory cytokines in the peripheral mononuclear blood cells (PBMCs). Supernatant from EGCG-pretreated and LPS-activated macrophage cultures was found to be less cytotoxic to neurons than that from non-EGCG-pretreated and LPS-activated macrophage cultures. Furthermore, EGCG treatment of neurons could inhibit LPS-induced production of reactive oxygen species (ROS). Thus EGCG represents a potent and useful neuroprotective agent for inflammation-mediated neurological disorders. |
| format | Article |
| id | doaj-art-38a5d0e531d748e39595dae4073b1644 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-38a5d0e531d748e39595dae4073b16442025-02-03T05:54:38ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/49623514962351Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated CytotoxicityJin-Biao Liu0Li Zhou1Yi-Zhong Wang2Xu Wang3Yu Zhou4Wen-Zhe Ho5Jie-Liang Li6Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, Wuhan 430071, ChinaAnimal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, Wuhan 430071, ChinaDepartment of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USADepartment of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USADepartment of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USAAnimal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, Wuhan 430071, ChinaDepartment of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USALipopolysaccharide- (LPS-) mediated systemic inflammation plays a critical role in neurodegenerative diseases. The present study was conducted to evaluate the protective effects of epigallocatechin gallate (EGCG), the major component in green tea, on LPS-mediated inflammation and neurotoxicity. LPS treatment of macrophages induced expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6). However, EGCG pretreatment of macrophages significantly inhibited LPS-mediated induction of these cytokines. In addition, EGCG significantly diminished LPS-induced inflammatory cytokines in the peripheral mononuclear blood cells (PBMCs). Supernatant from EGCG-pretreated and LPS-activated macrophage cultures was found to be less cytotoxic to neurons than that from non-EGCG-pretreated and LPS-activated macrophage cultures. Furthermore, EGCG treatment of neurons could inhibit LPS-induced production of reactive oxygen species (ROS). Thus EGCG represents a potent and useful neuroprotective agent for inflammation-mediated neurological disorders.http://dx.doi.org/10.1155/2016/4962351 |
| spellingShingle | Jin-Biao Liu Li Zhou Yi-Zhong Wang Xu Wang Yu Zhou Wen-Zhe Ho Jie-Liang Li Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity Journal of Immunology Research |
| title | Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity |
| title_full | Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity |
| title_fullStr | Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity |
| title_full_unstemmed | Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity |
| title_short | Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity |
| title_sort | neuroprotective activity of epigallocatechin gallate against lipopolysaccharide mediated cytotoxicity |
| url | http://dx.doi.org/10.1155/2016/4962351 |
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