Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae
Summary: Chordotonal organs are sensory structures containing ciliated neurons specialized in detecting mechanical forces. Here, we present a protocol for recording the neuronal activity of mechanically stimulated pentascolopidial chordotonal organs (lch5) of Drosophila larvae. We describe steps for...
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002278 |
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| author | Marek B. Körner Michal Mitro Dmitrij Ljaschenko Nicole Scholz |
| author_facet | Marek B. Körner Michal Mitro Dmitrij Ljaschenko Nicole Scholz |
| author_sort | Marek B. Körner |
| collection | DOAJ |
| description | Summary: Chordotonal organs are sensory structures containing ciliated neurons specialized in detecting mechanical forces. Here, we present a protocol for recording the neuronal activity of mechanically stimulated pentascolopidial chordotonal organs (lch5) of Drosophila larvae. We describe steps for dissecting larvae, measuring electrical activity of chordotonal neurons, and quantifying action currents. This protocol is useful for studying the biology of larval lch5 neurons, as well as the molecular mechanisms and components that contribute to their mechanosensitive properties.For complete details on the use and execution of this protocol, please refer to Bormann et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-38a031bd1b02430783f2debf1f8e8a77 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-38a031bd1b02430783f2debf1f8e8a772025-08-20T03:49:46ZengElsevierSTAR Protocols2666-16672025-06-016210382110.1016/j.xpro.2025.103821Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvaeMarek B. Körner0Michal Mitro1Dmitrij Ljaschenko2Nicole Scholz3Rudolf Schönheimer Institute of Biochemistry, Division of General Biochemistry, Medical Faculty, Leipzig University, 04103 Leipzig, GermanyRudolf Schönheimer Institute of Biochemistry, Division of General Biochemistry, Medical Faculty, Leipzig University, 04103 Leipzig, GermanyRudolf Schönheimer Institute of Biochemistry, Division of General Biochemistry, Medical Faculty, Leipzig University, 04103 Leipzig, Germany; Corresponding authorRudolf Schönheimer Institute of Biochemistry, Division of General Biochemistry, Medical Faculty, Leipzig University, 04103 Leipzig, Germany; Corresponding authorSummary: Chordotonal organs are sensory structures containing ciliated neurons specialized in detecting mechanical forces. Here, we present a protocol for recording the neuronal activity of mechanically stimulated pentascolopidial chordotonal organs (lch5) of Drosophila larvae. We describe steps for dissecting larvae, measuring electrical activity of chordotonal neurons, and quantifying action currents. This protocol is useful for studying the biology of larval lch5 neurons, as well as the molecular mechanisms and components that contribute to their mechanosensitive properties.For complete details on the use and execution of this protocol, please refer to Bormann et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002278Cell BiologyModel OrganismsNeuroscience |
| spellingShingle | Marek B. Körner Michal Mitro Dmitrij Ljaschenko Nicole Scholz Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae STAR Protocols Cell Biology Model Organisms Neuroscience |
| title | Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae |
| title_full | Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae |
| title_fullStr | Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae |
| title_full_unstemmed | Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae |
| title_short | Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae |
| title_sort | protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in drosophila larvae |
| topic | Cell Biology Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002278 |
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