Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene Production
ABSTRACT Yeast extract (YE) is a complex nutritional source associated with high performance on microbial production processes. However, its inherent compositional variability challenges its scalability. While prior efforts have focused on growth‐associated products, the dynamics of growth‐uncoupled...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-11-01
|
| Series: | Microbial Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1751-7915.70056 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850224741312364544 |
|---|---|
| author | Carlos Castillo‐Saldarriaga Christine N. S. Santos Stephen Sarria Parayil K. Ajikumar Ralf Takors |
| author_facet | Carlos Castillo‐Saldarriaga Christine N. S. Santos Stephen Sarria Parayil K. Ajikumar Ralf Takors |
| author_sort | Carlos Castillo‐Saldarriaga |
| collection | DOAJ |
| description | ABSTRACT Yeast extract (YE) is a complex nutritional source associated with high performance on microbial production processes. However, its inherent compositional variability challenges its scalability. While prior efforts have focused on growth‐associated products, the dynamics of growth‐uncoupled production, which leads to higher production rates and conversion yields, still need to be explored. This production scenario is common in large‐scale applications. This study presents a systematic approach to replace YE for the production of the terpene amorpha‐4,11‐diene in Escherichia coli. Sequential processing was successfully applied to identify glutamic acid, alanine, leucine, valine, isoleucine and glycine as the key amino acids (AAs) under slow‐growth conditions. Thoroughly applying biomass retention as part of sequential processing increased production capacity by 45% using these AAs instead of YE. Further studies, including flux balance analyses, targeted pyruvate as the common AA precursor. The optimized fed‐batch process feeding pyruvate with 0.09 gPyr h−1 enhanced amorpha‐4,11‐diene production by 37%, although adding only 1% carbon via pyruvate. Flux balance analysis revealed the criteria for optimum pyruvate feeding, for example, to prevent succinate secretion and maintain the NADH/NAD+ balance. These findings illustrate the interplay between media composition and metabolic activity and provide a successful guideline for identifying lean, best‐performing media for industrial applications. |
| format | Article |
| id | doaj-art-388e1bbcf1754c809bb68f94e409f427 |
| institution | OA Journals |
| issn | 1751-7915 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj-art-388e1bbcf1754c809bb68f94e409f4272025-08-20T02:05:32ZengWileyMicrobial Biotechnology1751-79152024-11-011711n/an/a10.1111/1751-7915.70056Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene ProductionCarlos Castillo‐Saldarriaga0Christine N. S. Santos1Stephen Sarria2Parayil K. Ajikumar3Ralf Takors4Institute of Biochemical Engineering University of Stuttgart Stuttgart GermanyManusBio Cambridge Massachusetts USAManusBio Cambridge Massachusetts USAManusBio Cambridge Massachusetts USAInstitute of Biochemical Engineering University of Stuttgart Stuttgart GermanyABSTRACT Yeast extract (YE) is a complex nutritional source associated with high performance on microbial production processes. However, its inherent compositional variability challenges its scalability. While prior efforts have focused on growth‐associated products, the dynamics of growth‐uncoupled production, which leads to higher production rates and conversion yields, still need to be explored. This production scenario is common in large‐scale applications. This study presents a systematic approach to replace YE for the production of the terpene amorpha‐4,11‐diene in Escherichia coli. Sequential processing was successfully applied to identify glutamic acid, alanine, leucine, valine, isoleucine and glycine as the key amino acids (AAs) under slow‐growth conditions. Thoroughly applying biomass retention as part of sequential processing increased production capacity by 45% using these AAs instead of YE. Further studies, including flux balance analyses, targeted pyruvate as the common AA precursor. The optimized fed‐batch process feeding pyruvate with 0.09 gPyr h−1 enhanced amorpha‐4,11‐diene production by 37%, although adding only 1% carbon via pyruvate. Flux balance analysis revealed the criteria for optimum pyruvate feeding, for example, to prevent succinate secretion and maintain the NADH/NAD+ balance. These findings illustrate the interplay between media composition and metabolic activity and provide a successful guideline for identifying lean, best‐performing media for industrial applications.https://doi.org/10.1111/1751-7915.70056amino acidscomplex mediaEscherichia colifermentationpyruvateterpenoids |
| spellingShingle | Carlos Castillo‐Saldarriaga Christine N. S. Santos Stephen Sarria Parayil K. Ajikumar Ralf Takors Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene Production Microbial Biotechnology amino acids complex media Escherichia coli fermentation pyruvate terpenoids |
| title | Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene Production |
| title_full | Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene Production |
| title_fullStr | Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene Production |
| title_full_unstemmed | Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene Production |
| title_short | Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha‐4,11‐Diene Production |
| title_sort | substitute yeast extract while maintaining performance showcase amorpha 4 11 diene production |
| topic | amino acids complex media Escherichia coli fermentation pyruvate terpenoids |
| url | https://doi.org/10.1111/1751-7915.70056 |
| work_keys_str_mv | AT carloscastillosaldarriaga substituteyeastextractwhilemaintainingperformanceshowcaseamorpha411dieneproduction AT christinenssantos substituteyeastextractwhilemaintainingperformanceshowcaseamorpha411dieneproduction AT stephensarria substituteyeastextractwhilemaintainingperformanceshowcaseamorpha411dieneproduction AT parayilkajikumar substituteyeastextractwhilemaintainingperformanceshowcaseamorpha411dieneproduction AT ralftakors substituteyeastextractwhilemaintainingperformanceshowcaseamorpha411dieneproduction |