The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensii
L-carnitine (LC) is a natural antioxidant that plays an important role in improving the antioxidant capacity of organisms and regulating the immune response. The aim of this study was to clarify the effect of LC on immune regulation in Pinctada fucata martensii. LC was injected into P. f. martensii...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000687 |
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| author | Weijiang Liao Jianuo Wu Xiaojun Liu Mingtao Zhang Ming Zhang Yu Jiao Qingheng Wang Chuangye Yang Yuewen Deng |
| author_facet | Weijiang Liao Jianuo Wu Xiaojun Liu Mingtao Zhang Ming Zhang Yu Jiao Qingheng Wang Chuangye Yang Yuewen Deng |
| author_sort | Weijiang Liao |
| collection | DOAJ |
| description | L-carnitine (LC) is a natural antioxidant that plays an important role in improving the antioxidant capacity of organisms and regulating the immune response. The aim of this study was to clarify the effect of LC on immune regulation in Pinctada fucata martensii. LC was injected into P. f. martensii prior to lipopolysaccharide (LPS) stimulation or transplantation surgery. Serum immune-related enzyme activities, hemocyte apoptosis, and nucleus retention and survival rate of the transplanted pearl oysters were analyzed. LC pretreatment prior to LPS injection can lower the serum content of TNF-α and IL-17, enhance the activities of SOD and CAT, block PI3K-Akt and TNF signaling pathways, and limit hemocyte apoptosis. LC injection prior to transplantation surgery also reduced the content of TNF-α and IL-17, enhanced the antioxidant activity of serum, inhibited the NF-kappa B signal pathway, and increased the nucleus retention rate; however, LC injection had no effect on the survival rate of the transplanted oysters. These findings indicate that LC could inhibit the immune response induced by LPS and transplantation surgery and have implications for the development of immunosuppressive agents. |
| format | Article |
| id | doaj-art-3889d3bea82e4deba0dace78f34e6085 |
| institution | DOAJ |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-3889d3bea82e4deba0dace78f34e60852025-08-20T02:59:52ZengElsevierAquaculture Reports2352-51342025-04-014110268210.1016/j.aqrep.2025.102682The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensiiWeijiang Liao0Jianuo Wu1Xiaojun Liu2Mingtao Zhang3Ming Zhang4Yu Jiao5Qingheng Wang6Chuangye Yang7Yuewen Deng8Fishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Corresponding author at: Fishery College, Guangdong Ocean University, Zhanjiang 524088, China.Fishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Zhanjiang 524088, China; Guangdong Marine Ecology Early Warning and Monitoring Laboratory, Zhanjiang 524088, ChinaL-carnitine (LC) is a natural antioxidant that plays an important role in improving the antioxidant capacity of organisms and regulating the immune response. The aim of this study was to clarify the effect of LC on immune regulation in Pinctada fucata martensii. LC was injected into P. f. martensii prior to lipopolysaccharide (LPS) stimulation or transplantation surgery. Serum immune-related enzyme activities, hemocyte apoptosis, and nucleus retention and survival rate of the transplanted pearl oysters were analyzed. LC pretreatment prior to LPS injection can lower the serum content of TNF-α and IL-17, enhance the activities of SOD and CAT, block PI3K-Akt and TNF signaling pathways, and limit hemocyte apoptosis. LC injection prior to transplantation surgery also reduced the content of TNF-α and IL-17, enhanced the antioxidant activity of serum, inhibited the NF-kappa B signal pathway, and increased the nucleus retention rate; however, LC injection had no effect on the survival rate of the transplanted oysters. These findings indicate that LC could inhibit the immune response induced by LPS and transplantation surgery and have implications for the development of immunosuppressive agents.http://www.sciencedirect.com/science/article/pii/S2352513425000687Pinctada fucata martensiiPearl formationTransplantationL-carnitine |
| spellingShingle | Weijiang Liao Jianuo Wu Xiaojun Liu Mingtao Zhang Ming Zhang Yu Jiao Qingheng Wang Chuangye Yang Yuewen Deng The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensii Aquaculture Reports Pinctada fucata martensii Pearl formation Transplantation L-carnitine |
| title | The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensii |
| title_full | The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensii |
| title_fullStr | The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensii |
| title_full_unstemmed | The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensii |
| title_short | The immunomodulatory effects of L-carnitine on the immune responses induced by LPS and transplantation in the pearl oyster Pinctada fucata martensii |
| title_sort | immunomodulatory effects of l carnitine on the immune responses induced by lps and transplantation in the pearl oyster pinctada fucata martensii |
| topic | Pinctada fucata martensii Pearl formation Transplantation L-carnitine |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000687 |
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