Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy Goats
<i>Lentinus edodes</i> (LE) is a nutrient-rich medicinal fungus with potential applications in animal nutrition; however, its effects on dairy goats remain underexplored. This study investigated the impact of dietary LE addition on rumen microbiota, metabolic profiles, serum immunity, an...
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2025-02-01
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| author | Huijun Shen Mengyu Wang Yong Ning Yiqi Zhao Baiji Danzeng Kaixin Li Huaiping Shi Weijuan Li |
| author_facet | Huijun Shen Mengyu Wang Yong Ning Yiqi Zhao Baiji Danzeng Kaixin Li Huaiping Shi Weijuan Li |
| author_sort | Huijun Shen |
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| description | <i>Lentinus edodes</i> (LE) is a nutrient-rich medicinal fungus with potential applications in animal nutrition; however, its effects on dairy goats remain underexplored. This study investigated the impact of dietary LE addition on rumen microbiota, metabolic profiles, serum immunity, and milk quality in lactating dairy goats. Twenty Saanen goats were randomly assigned to a control group (CON, basal diet) or an LE group (basal diet + 25 g <i>Lentinus edodes</i>) for 56 days. Rumen fluid, serum, and milk samples were analyzed using 16S rDNA sequencing, metabolomics, and biochemical assays. The results showed that the addition of LE altered the microbial composition, decreasing the abundance of fibrobacterial flora and <i>Treponema</i> (<i>p</i> < 0.05) while increasing VadinHA49. Metabolomic analysis revealed elevated fumaric acid, lysophospholipids (LysoPE, LysoPG), and D-quinic acid in the rumen (<i>p</i> < 0.05). Serum immunoglobulin A (IgA), immunoglobulin G (IgG), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and tumor necrosis factor-α (TNF-α) levels were significantly enhanced (<i>p</i> < 0.05). The milk somatic cell count (SCC) was reduced by 52.8% (<i>p</i> = 0.005) at day 56, and protein, fat, and total solids increased significantly (<i>p</i> < 0.05). Enrichment of functional metabolites such as D-arabitol and D-tryptophan in milk further highlighted LE’s role in improving product value. These findings demonstrate that LE optimizes rumen flora, enhances antioxidant and immune functions, and improves milk quality, supporting its use as a functional feed additive for dairy goats. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-38890fee85aa4105bb48dbc4c6de69dc2025-08-20T02:04:34ZengMDPI AGAnimals2076-26152025-02-0115567610.3390/ani15050676Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy GoatsHuijun Shen0Mengyu Wang1Yong Ning2Yiqi Zhao3Baiji Danzeng4Kaixin Li5Huaiping Shi6Weijuan Li7College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650224, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650224, China<i>Lentinus edodes</i> (LE) is a nutrient-rich medicinal fungus with potential applications in animal nutrition; however, its effects on dairy goats remain underexplored. This study investigated the impact of dietary LE addition on rumen microbiota, metabolic profiles, serum immunity, and milk quality in lactating dairy goats. Twenty Saanen goats were randomly assigned to a control group (CON, basal diet) or an LE group (basal diet + 25 g <i>Lentinus edodes</i>) for 56 days. Rumen fluid, serum, and milk samples were analyzed using 16S rDNA sequencing, metabolomics, and biochemical assays. The results showed that the addition of LE altered the microbial composition, decreasing the abundance of fibrobacterial flora and <i>Treponema</i> (<i>p</i> < 0.05) while increasing VadinHA49. Metabolomic analysis revealed elevated fumaric acid, lysophospholipids (LysoPE, LysoPG), and D-quinic acid in the rumen (<i>p</i> < 0.05). Serum immunoglobulin A (IgA), immunoglobulin G (IgG), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and tumor necrosis factor-α (TNF-α) levels were significantly enhanced (<i>p</i> < 0.05). The milk somatic cell count (SCC) was reduced by 52.8% (<i>p</i> = 0.005) at day 56, and protein, fat, and total solids increased significantly (<i>p</i> < 0.05). Enrichment of functional metabolites such as D-arabitol and D-tryptophan in milk further highlighted LE’s role in improving product value. These findings demonstrate that LE optimizes rumen flora, enhances antioxidant and immune functions, and improves milk quality, supporting its use as a functional feed additive for dairy goats.https://www.mdpi.com/2076-2615/15/5/676<i>Lentinus edodes</i>rumen microbiotametabolomicsimmune enhancementmilk compositionoxidative stress |
| spellingShingle | Huijun Shen Mengyu Wang Yong Ning Yiqi Zhao Baiji Danzeng Kaixin Li Huaiping Shi Weijuan Li Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy Goats Animals <i>Lentinus edodes</i> rumen microbiota metabolomics immune enhancement milk composition oxidative stress |
| title | Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy Goats |
| title_full | Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy Goats |
| title_fullStr | Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy Goats |
| title_full_unstemmed | Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy Goats |
| title_short | Effect of Dietary Addition of <i>Lentinus edodes</i> on Rumen Flora, Lactation, and Health of Dairy Goats |
| title_sort | effect of dietary addition of i lentinus edodes i on rumen flora lactation and health of dairy goats |
| topic | <i>Lentinus edodes</i> rumen microbiota metabolomics immune enhancement milk composition oxidative stress |
| url | https://www.mdpi.com/2076-2615/15/5/676 |
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