5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i>
Endophytic fungi are valuable sources of bioactive secondary metabolites, with potential applications in pharmaceutical and agricultural fields. This study investigates the metabolic potential of <i>Diaporthe hongkongensis</i>, an endophytic fungus isolated from <i>Minquartia guian...
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2025-03-01
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| author | Andrei da Silva Alexandre Luana Lopes Casas David Ribeiro da Silva Cecilia Veronica Nunez |
| author_facet | Andrei da Silva Alexandre Luana Lopes Casas David Ribeiro da Silva Cecilia Veronica Nunez |
| author_sort | Andrei da Silva Alexandre |
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| description | Endophytic fungi are valuable sources of bioactive secondary metabolites, with potential applications in pharmaceutical and agricultural fields. This study investigates the metabolic potential of <i>Diaporthe hongkongensis</i>, an endophytic fungus isolated from <i>Minquartia guianensis</i>. To date, no secondary metabolites have been identified from this species, highlighting the novelty of this research and its contribution to understanding the chemical diversity of endophytic fungi. The fungus was cultivated on parboiled rice under static and dark conditions for 28 days, leading to the isolation of the following three compounds: 5,6-dihydro-5,6-epoxymultiplolide A (<b>1</b>), cytosporone C (<b>2</b>), and uridine (<b>3</b>). Structural identification was carried out using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The results revealed the metabolic versatility of <i>D. hongkongensis</i>, as demonstrated by its ability to produce structurally diverse substances with biological relevance. Hence, it describes the first isolation of secondary metabolites from the endophytic fungus <i>D. hongkongensis</i>, marking a significant step in understanding its chemical profile. The identification of a known antifungal compound and a lactone derivative underscores the biosynthetic potential of this endophytic fungus, while the isolation of a nucleoside expands the chemical repertoire of fungal metabolites, suggesting possible roles in cellular metabolism and stress adaptation. These findings highlight the role of endophytic fungi as prolific sources of structurally diverse and potentially bioactive natural products, supporting further exploration of their biotechnological applications. |
| format | Article |
| id | doaj-art-387f96e32b694bbcbc97caf68de5358a |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
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| record_format | Article |
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| spelling | doaj-art-387f96e32b694bbcbc97caf68de5358a2025-08-20T02:28:24ZengMDPI AGMicroorganisms2076-26072025-03-0113479210.3390/microorganisms130407925,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i>Andrei da Silva Alexandre0Luana Lopes Casas1David Ribeiro da Silva2Cecilia Veronica Nunez3Bioprospecting and Biotechnology Laboratory, Technology and Innovation Coordination, National Institute of Amazonian Research, Manaus 69067-375, AM, BrazilBioprospecting and Biotechnology Laboratory, Technology and Innovation Coordination, National Institute of Amazonian Research, Manaus 69067-375, AM, BrazilBioprospecting and Biotechnology Laboratory, Technology and Innovation Coordination, National Institute of Amazonian Research, Manaus 69067-375, AM, BrazilBioprospecting and Biotechnology Laboratory, Technology and Innovation Coordination, National Institute of Amazonian Research, Manaus 69067-375, AM, BrazilEndophytic fungi are valuable sources of bioactive secondary metabolites, with potential applications in pharmaceutical and agricultural fields. This study investigates the metabolic potential of <i>Diaporthe hongkongensis</i>, an endophytic fungus isolated from <i>Minquartia guianensis</i>. To date, no secondary metabolites have been identified from this species, highlighting the novelty of this research and its contribution to understanding the chemical diversity of endophytic fungi. The fungus was cultivated on parboiled rice under static and dark conditions for 28 days, leading to the isolation of the following three compounds: 5,6-dihydro-5,6-epoxymultiplolide A (<b>1</b>), cytosporone C (<b>2</b>), and uridine (<b>3</b>). Structural identification was carried out using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The results revealed the metabolic versatility of <i>D. hongkongensis</i>, as demonstrated by its ability to produce structurally diverse substances with biological relevance. Hence, it describes the first isolation of secondary metabolites from the endophytic fungus <i>D. hongkongensis</i>, marking a significant step in understanding its chemical profile. The identification of a known antifungal compound and a lactone derivative underscores the biosynthetic potential of this endophytic fungus, while the isolation of a nucleoside expands the chemical repertoire of fungal metabolites, suggesting possible roles in cellular metabolism and stress adaptation. These findings highlight the role of endophytic fungi as prolific sources of structurally diverse and potentially bioactive natural products, supporting further exploration of their biotechnological applications.https://www.mdpi.com/2076-2607/13/4/792fungusparboiled ricesecondary metabolitesNMR spectroscopymultiplolidepolyketide |
| spellingShingle | Andrei da Silva Alexandre Luana Lopes Casas David Ribeiro da Silva Cecilia Veronica Nunez 5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i> Microorganisms fungus parboiled rice secondary metabolites NMR spectroscopy multiplolide polyketide |
| title | 5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i> |
| title_full | 5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i> |
| title_fullStr | 5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i> |
| title_full_unstemmed | 5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i> |
| title_short | 5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production by <i>Diaporthe hongkongensis</i>, an Endophytic Fungus from <i>Minquartia guianensis</i> |
| title_sort | 5 6 dihydro 5 6 epoxymultiplolide a cytosporone c and uridine production by i diaporthe hongkongensis i an endophytic fungus from i minquartia guianensis i |
| topic | fungus parboiled rice secondary metabolites NMR spectroscopy multiplolide polyketide |
| url | https://www.mdpi.com/2076-2607/13/4/792 |
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