New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit

Rotavirus species A (RVA) is a major cause of acute viral gastroenteritis in young humans and diverse animal species. The study of the genetic characteristics of RVAs that infect rabbits (Oryctolagus cuniculus) (lapine strain [LRV]) has been limited, and, to date, the most common and epidemiological...

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Main Authors: Emmanuel Reynoso-Utrera, Linda Guiliana Bautista-Gómez, Salvador Fonseca-Coronado, Juan Diego Pérez-de la Rosa, Valeria Jazmín Rodríguez-Villavicencio, Camilo Romero-Núñez, Ariadna Flores-Ortega, Pedro Abel Hernández-García, José Simón Martínez-Castañeda
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Viruses
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Online Access:https://www.mdpi.com/1999-4915/16/11/1729
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author Emmanuel Reynoso-Utrera
Linda Guiliana Bautista-Gómez
Salvador Fonseca-Coronado
Juan Diego Pérez-de la Rosa
Valeria Jazmín Rodríguez-Villavicencio
Camilo Romero-Núñez
Ariadna Flores-Ortega
Pedro Abel Hernández-García
José Simón Martínez-Castañeda
author_facet Emmanuel Reynoso-Utrera
Linda Guiliana Bautista-Gómez
Salvador Fonseca-Coronado
Juan Diego Pérez-de la Rosa
Valeria Jazmín Rodríguez-Villavicencio
Camilo Romero-Núñez
Ariadna Flores-Ortega
Pedro Abel Hernández-García
José Simón Martínez-Castañeda
author_sort Emmanuel Reynoso-Utrera
collection DOAJ
description Rotavirus species A (RVA) is a major cause of acute viral gastroenteritis in young humans and diverse animal species. The study of the genetic characteristics of RVAs that infect rabbits (Oryctolagus cuniculus) (lapine strain [LRV]) has been limited, and, to date, the most common and epidemiologically important combinations of G/P genotypes in rabbits have been reported to be G3 P[14] and G3 P[22]. In this study, a rotavirus species A detected from an outbreak of enteritis in a Mexican commercial rabbitry was genotypically characterized. Based on sequence and phylogenetic analysis of the VP7 and VP4 genes, the strain identified in this study (C-3/15) demonstrated a G3 P[8] genotype of rotavirus, which had not previously been reported in rabbits. Moreover, both genes were closely related to human, not lapine, rotaviruses. The G3 genotype has been reported in a wide variety of hosts, including humans and rabbits, whereas the P[8] genotype has only been reported in humans. Because this combination of genotypes has never been identified in rabbits, it is proposed that the finding presented here is possibly the result of an interspecies transmission event. This is the first work to study the molecular characteristics of rotaviruses in rabbits in Mexico, as well as the identification of human G3 and P[8] genotypes in a rabbit with enteric disease.
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spelling doaj-art-387e30f97e3e44239a2f7c2af4524fcb2025-08-20T01:54:08ZengMDPI AGViruses1999-49152024-11-011611172910.3390/v16111729New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric RabbitEmmanuel Reynoso-Utrera0Linda Guiliana Bautista-Gómez1Salvador Fonseca-Coronado2Juan Diego Pérez-de la Rosa3Valeria Jazmín Rodríguez-Villavicencio4Camilo Romero-Núñez5Ariadna Flores-Ortega6Pedro Abel Hernández-García7José Simón Martínez-Castañeda8Laboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, MexicoLaboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, MexicoLaboratorio de Investigación en Inmunología y Salud Pública, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastian Xhala 54714, MexicoLaboratorio de Diagnóstico Molecular JGG & RNL, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoLaboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, MexicoHospital Veterinario DERMAVET, Ciudad de México 09510, MexicoLaboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, MexicoLaboratorio Multidisciplinario en Investigación, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, MexicoCentro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca 50000, MexicoRotavirus species A (RVA) is a major cause of acute viral gastroenteritis in young humans and diverse animal species. The study of the genetic characteristics of RVAs that infect rabbits (Oryctolagus cuniculus) (lapine strain [LRV]) has been limited, and, to date, the most common and epidemiologically important combinations of G/P genotypes in rabbits have been reported to be G3 P[14] and G3 P[22]. In this study, a rotavirus species A detected from an outbreak of enteritis in a Mexican commercial rabbitry was genotypically characterized. Based on sequence and phylogenetic analysis of the VP7 and VP4 genes, the strain identified in this study (C-3/15) demonstrated a G3 P[8] genotype of rotavirus, which had not previously been reported in rabbits. Moreover, both genes were closely related to human, not lapine, rotaviruses. The G3 genotype has been reported in a wide variety of hosts, including humans and rabbits, whereas the P[8] genotype has only been reported in humans. Because this combination of genotypes has never been identified in rabbits, it is proposed that the finding presented here is possibly the result of an interspecies transmission event. This is the first work to study the molecular characteristics of rotaviruses in rabbits in Mexico, as well as the identification of human G3 and P[8] genotypes in a rabbit with enteric disease.https://www.mdpi.com/1999-4915/16/11/1729rotavirusrabbitgenotypic characterizationG3P[8]Mexico
spellingShingle Emmanuel Reynoso-Utrera
Linda Guiliana Bautista-Gómez
Salvador Fonseca-Coronado
Juan Diego Pérez-de la Rosa
Valeria Jazmín Rodríguez-Villavicencio
Camilo Romero-Núñez
Ariadna Flores-Ortega
Pedro Abel Hernández-García
José Simón Martínez-Castañeda
New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit
Viruses
rotavirus
rabbit
genotypic characterization
G3P[8]
Mexico
title New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit
title_full New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit
title_fullStr New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit
title_full_unstemmed New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit
title_short New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit
title_sort new genotype g3 p 8 of rotavirus identified in a mexican gastroenteric rabbit
topic rotavirus
rabbit
genotypic characterization
G3P[8]
Mexico
url https://www.mdpi.com/1999-4915/16/11/1729
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