Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala Injury
Amygdala is involved in the fear memory that recognizes certain environmental cues predicting threatening events. Manipulation of neurotransmission within the amygdala affects the expression of conditioned and unconditioned emotional memories such as fear freezing behaviour. We previously demonstrat...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2016/8521297 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850105909182726144 |
|---|---|
| author | Kyungha Shin Yeseul Cha Kwang Sei Kim Ehn-Kyoung Choi Youngjin Choi Haiyu Guo Young-Hwan Ban Jong-Choon Kim Dongsun Park Yun-Bae Kim |
| author_facet | Kyungha Shin Yeseul Cha Kwang Sei Kim Ehn-Kyoung Choi Youngjin Choi Haiyu Guo Young-Hwan Ban Jong-Choon Kim Dongsun Park Yun-Bae Kim |
| author_sort | Kyungha Shin |
| collection | DOAJ |
| description | Amygdala is involved in the fear memory that recognizes certain environmental cues predicting threatening events. Manipulation of neurotransmission within the amygdala affects the expression of conditioned and unconditioned emotional memories such as fear freezing behaviour. We previously demonstrated that F3.ChAT human neural stem cells (NSCs) overexpressing choline acetyltransferase (ChAT) improve cognitive function of Alzheimer’s disease model rats with hippocampal or cholinergic nerve injuries by increasing acetylcholine (ACh) level. In the present study, we examined the effect of F3.ChAT cells on the deficit of unconditioned fear freezing. Rats given N-methyl-d-aspartate (NMDA) in their amygdala 2 weeks prior to cat odor exposure displayed very short resting (freezing) time compared to normal animals. NMDA induced neuronal degeneration in the amygdala, leading to a decreased ACh concentration in cerebrospinal fluid. However, intracerebroventricular transplantation of F3.ChAT cells attenuated amygdala lesions 4 weeks after transplantation. The transplanted cells were found in the NMDA-injury sites and produced ChAT protein. In addition, F3.ChAT-receiving rats recuperated freezing time staying remote from the cat odor source, according to the recovery of brain ACh concentration. The results indicate that human NSCs overexpressing ChAT may facilitate retrieval of unconditioned fear memory by increasing ACh level. |
| format | Article |
| id | doaj-art-386e0cfabbe848c09748d0a0d0a35cd7 |
| institution | OA Journals |
| issn | 0953-4180 1875-8584 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-386e0cfabbe848c09748d0a0d0a35cd72025-08-20T02:38:58ZengWileyBehavioural Neurology0953-41801875-85842016-01-01201610.1155/2016/85212978521297Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala InjuryKyungha Shin0Yeseul Cha1Kwang Sei Kim2Ehn-Kyoung Choi3Youngjin Choi4Haiyu Guo5Young-Hwan Ban6Jong-Choon Kim7Dongsun Park8Yun-Bae Kim9College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaAmygdala is involved in the fear memory that recognizes certain environmental cues predicting threatening events. Manipulation of neurotransmission within the amygdala affects the expression of conditioned and unconditioned emotional memories such as fear freezing behaviour. We previously demonstrated that F3.ChAT human neural stem cells (NSCs) overexpressing choline acetyltransferase (ChAT) improve cognitive function of Alzheimer’s disease model rats with hippocampal or cholinergic nerve injuries by increasing acetylcholine (ACh) level. In the present study, we examined the effect of F3.ChAT cells on the deficit of unconditioned fear freezing. Rats given N-methyl-d-aspartate (NMDA) in their amygdala 2 weeks prior to cat odor exposure displayed very short resting (freezing) time compared to normal animals. NMDA induced neuronal degeneration in the amygdala, leading to a decreased ACh concentration in cerebrospinal fluid. However, intracerebroventricular transplantation of F3.ChAT cells attenuated amygdala lesions 4 weeks after transplantation. The transplanted cells were found in the NMDA-injury sites and produced ChAT protein. In addition, F3.ChAT-receiving rats recuperated freezing time staying remote from the cat odor source, according to the recovery of brain ACh concentration. The results indicate that human NSCs overexpressing ChAT may facilitate retrieval of unconditioned fear memory by increasing ACh level.http://dx.doi.org/10.1155/2016/8521297 |
| spellingShingle | Kyungha Shin Yeseul Cha Kwang Sei Kim Ehn-Kyoung Choi Youngjin Choi Haiyu Guo Young-Hwan Ban Jong-Choon Kim Dongsun Park Yun-Bae Kim Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala Injury Behavioural Neurology |
| title | Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala Injury |
| title_full | Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala Injury |
| title_fullStr | Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala Injury |
| title_full_unstemmed | Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala Injury |
| title_short | Human Neural Stem Cells Overexpressing Choline Acetyltransferase Restore Unconditioned Fear in Rats with Amygdala Injury |
| title_sort | human neural stem cells overexpressing choline acetyltransferase restore unconditioned fear in rats with amygdala injury |
| url | http://dx.doi.org/10.1155/2016/8521297 |
| work_keys_str_mv | AT kyunghashin humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT yeseulcha humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT kwangseikim humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT ehnkyoungchoi humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT youngjinchoi humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT haiyuguo humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT younghwanban humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT jongchoonkim humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT dongsunpark humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury AT yunbaekim humanneuralstemcellsoverexpressingcholineacetyltransferaserestoreunconditionedfearinratswithamygdalainjury |