Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method

Ceftriaxone sodium, an antimicrobial agent that plays an important role in clinical practice, is successfully used to treat infections caused by most Gram-positive and Gram-negative organisms. Since there are few rapid analytical methods for ceftriaxone analysis to use in the pharmaceutical routine,...

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Main Authors: Patrícia Vidal de Aléssio, Ana Carolina Kogawa, Hérida Regina Nunes Salgado
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2017/7530242
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author Patrícia Vidal de Aléssio
Ana Carolina Kogawa
Hérida Regina Nunes Salgado
author_facet Patrícia Vidal de Aléssio
Ana Carolina Kogawa
Hérida Regina Nunes Salgado
author_sort Patrícia Vidal de Aléssio
collection DOAJ
description Ceftriaxone sodium, an antimicrobial agent that plays an important role in clinical practice, is successfully used to treat infections caused by most Gram-positive and Gram-negative organisms. Since there are few rapid analytical methods for ceftriaxone analysis to use in the pharmaceutical routine, the aim of this research was to develop a new method able to quantify this cephalosporin. Therefore, a sensitive, rapid, simple UV spectrophotometric method for the determination and quantification of ceftriaxone sodium was proposed. The UV detector was set at 241 nm. Beer’s law obeyed the concentration range of 10–20 µg mL−1. Statistical comparison of the results with a well-established reported method showed excellent agreement and proved that there is no significant difference in the accuracy and precision. Intra- and interday variability for the method were less than 2% relative standard deviation. The proposed method was applied to the determination of the examined drugs in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise, and reproducible as the official methods.
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issn 2090-8865
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language English
publishDate 2017-01-01
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series Journal of Analytical Methods in Chemistry
spelling doaj-art-3867d53addbe4e32b59094c4e8c5acef2025-08-20T02:38:58ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/75302427530242Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric MethodPatrícia Vidal de Aléssio0Ana Carolina Kogawa1Hérida Regina Nunes Salgado2Department of Pharmaceutics, School of Pharmaceutical Sciences of Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Pharmaceutics, School of Pharmaceutical Sciences of Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Pharmaceutics, School of Pharmaceutical Sciences of Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilCeftriaxone sodium, an antimicrobial agent that plays an important role in clinical practice, is successfully used to treat infections caused by most Gram-positive and Gram-negative organisms. Since there are few rapid analytical methods for ceftriaxone analysis to use in the pharmaceutical routine, the aim of this research was to develop a new method able to quantify this cephalosporin. Therefore, a sensitive, rapid, simple UV spectrophotometric method for the determination and quantification of ceftriaxone sodium was proposed. The UV detector was set at 241 nm. Beer’s law obeyed the concentration range of 10–20 µg mL−1. Statistical comparison of the results with a well-established reported method showed excellent agreement and proved that there is no significant difference in the accuracy and precision. Intra- and interday variability for the method were less than 2% relative standard deviation. The proposed method was applied to the determination of the examined drugs in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise, and reproducible as the official methods.http://dx.doi.org/10.1155/2017/7530242
spellingShingle Patrícia Vidal de Aléssio
Ana Carolina Kogawa
Hérida Regina Nunes Salgado
Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method
Journal of Analytical Methods in Chemistry
title Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method
title_full Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method
title_fullStr Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method
title_full_unstemmed Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method
title_short Quality of Ceftriaxone Sodium in Lyophilized Powder for Injection Evaluated by Clean, Fast, and Efficient Spectrophotometric Method
title_sort quality of ceftriaxone sodium in lyophilized powder for injection evaluated by clean fast and efficient spectrophotometric method
url http://dx.doi.org/10.1155/2017/7530242
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AT anacarolinakogawa qualityofceftriaxonesodiuminlyophilizedpowderforinjectionevaluatedbycleanfastandefficientspectrophotometricmethod
AT heridareginanunessalgado qualityofceftriaxonesodiuminlyophilizedpowderforinjectionevaluatedbycleanfastandefficientspectrophotometricmethod