Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant Activity

In this study, an improved method combining natural surfactants with a solvent–antisolvent precipitation technique was developed to prepare highly effective nano-sized tangeretin particles. Various natural surfactants were tested and compared, and the formulation was optimized using Plackett–Burman...

Full description

Saved in:
Bibliographic Details
Main Authors: Yan Huang, Wenxuan Huang, Xiaonan Zhang, Zhiwei Liu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Nanomaterials
Subjects:
Online Access:https://www.mdpi.com/2079-4991/15/11/791
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849722287490596864
author Yan Huang
Wenxuan Huang
Xiaonan Zhang
Zhiwei Liu
author_facet Yan Huang
Wenxuan Huang
Xiaonan Zhang
Zhiwei Liu
author_sort Yan Huang
collection DOAJ
description In this study, an improved method combining natural surfactants with a solvent–antisolvent precipitation technique was developed to prepare highly effective nano-sized tangeretin particles. Various natural surfactants were tested and compared, and the formulation was optimized using Plackett–Burman and Box–Behnken design methodologies. The optimal preparation conditions were identified as follows: a tangeretin–dimethyl sulfoxide (DMSO) solution concentration of 5.23 mg/mL, surfactant concentration of 4.72%, and a rotor diameter of 20 mm. Under these conditions, uniform nano-tangeretin particles with an average size of 428.73 ± 30.25 nm were successfully produced. The preparation process significantly reduced particle size without chemical structure of tangeretin, as confirmed by spectral analysis. Importantly, the free radical scavenging activity of the nano-tangeretin was markedly enhanced, showing 65.4% increase in DPPH radical inhibition compared to the unprocessed powder. These results demonstrate that the proposed method can improve the bioactivity and dispersibility of tangeretin, providing a valuable strategy for the efficient utilization and industrial-scale production of bioactive compounds from natural resources.
format Article
id doaj-art-3864af9dc18146eeaff554c060c6cf1b
institution DOAJ
issn 2079-4991
language English
publishDate 2025-05-01
publisher MDPI AG
record_format Article
series Nanomaterials
spelling doaj-art-3864af9dc18146eeaff554c060c6cf1b2025-08-20T03:11:22ZengMDPI AGNanomaterials2079-49912025-05-01151179110.3390/nano15110791Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant ActivityYan Huang0Wenxuan Huang1Xiaonan Zhang2Zhiwei Liu3Life Science College, Jiaying University, Meizhou 514000, ChinaLife Science College, Jiaying University, Meizhou 514000, ChinaLife Science College, Jiaying University, Meizhou 514000, ChinaLife Science College, Jiaying University, Meizhou 514000, ChinaIn this study, an improved method combining natural surfactants with a solvent–antisolvent precipitation technique was developed to prepare highly effective nano-sized tangeretin particles. Various natural surfactants were tested and compared, and the formulation was optimized using Plackett–Burman and Box–Behnken design methodologies. The optimal preparation conditions were identified as follows: a tangeretin–dimethyl sulfoxide (DMSO) solution concentration of 5.23 mg/mL, surfactant concentration of 4.72%, and a rotor diameter of 20 mm. Under these conditions, uniform nano-tangeretin particles with an average size of 428.73 ± 30.25 nm were successfully produced. The preparation process significantly reduced particle size without chemical structure of tangeretin, as confirmed by spectral analysis. Importantly, the free radical scavenging activity of the nano-tangeretin was markedly enhanced, showing 65.4% increase in DPPH radical inhibition compared to the unprocessed powder. These results demonstrate that the proposed method can improve the bioactivity and dispersibility of tangeretin, providing a valuable strategy for the efficient utilization and industrial-scale production of bioactive compounds from natural resources.https://www.mdpi.com/2079-4991/15/11/791natural surfactantsnano-tangeretin in particlessolvent–antisolvent precipitationantioxidant activity
spellingShingle Yan Huang
Wenxuan Huang
Xiaonan Zhang
Zhiwei Liu
Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant Activity
Nanomaterials
natural surfactants
nano-tangeretin in particles
solvent–antisolvent precipitation
antioxidant activity
title Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant Activity
title_full Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant Activity
title_fullStr Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant Activity
title_full_unstemmed Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant Activity
title_short Optimization of Nano-Tangeretin Recrystallization via Natural Surfactants in the Antisolvent Precipitation Process: Physicochemical Characterization and Antioxidant Activity
title_sort optimization of nano tangeretin recrystallization via natural surfactants in the antisolvent precipitation process physicochemical characterization and antioxidant activity
topic natural surfactants
nano-tangeretin in particles
solvent–antisolvent precipitation
antioxidant activity
url https://www.mdpi.com/2079-4991/15/11/791
work_keys_str_mv AT yanhuang optimizationofnanotangeretinrecrystallizationvianaturalsurfactantsintheantisolventprecipitationprocessphysicochemicalcharacterizationandantioxidantactivity
AT wenxuanhuang optimizationofnanotangeretinrecrystallizationvianaturalsurfactantsintheantisolventprecipitationprocessphysicochemicalcharacterizationandantioxidantactivity
AT xiaonanzhang optimizationofnanotangeretinrecrystallizationvianaturalsurfactantsintheantisolventprecipitationprocessphysicochemicalcharacterizationandantioxidantactivity
AT zhiweiliu optimizationofnanotangeretinrecrystallizationvianaturalsurfactantsintheantisolventprecipitationprocessphysicochemicalcharacterizationandantioxidantactivity