Protocol for purifying and reconstituting a vacuole membrane transporter Ypq1 into proteoliposomes

Protocol for purifying and reconstituting a vacuole membrane transporter Ypq1 into proteoliposomes

Summary: Studying the biochemical function of membrane transporters is important in understanding the biology of transporter-laden organelles such as lysosomes and vacuoles. We present a protocol for overexpressing, purifying, and reconstituting a vacuole membrane transporter Ypq1 into proteoliposom...

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Bibliographic Details
Main Authors: Felichi Mae Arines, Aleksander Wielenga, Randy B. Stockbridge, Ming Li
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell Membrane
Protein Biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006488
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