AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism
Abstract Background Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer with a high metastatic rate and high mortality rate. The molecular mechanism of ccRCC development, however, needs further study. Aurora kinase B (AURKB) functions as an important oncogene in vario...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-024-01352-y |
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| _version_ | 1832571505112776704 |
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| author | Yang Yang Dan Li Zhigang Liu Kai Zhou Wenxing Li Yanqi Yang Ruifang Sun Yulong Li |
| author_facet | Yang Yang Dan Li Zhigang Liu Kai Zhou Wenxing Li Yanqi Yang Ruifang Sun Yulong Li |
| author_sort | Yang Yang |
| collection | DOAJ |
| description | Abstract Background Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer with a high metastatic rate and high mortality rate. The molecular mechanism of ccRCC development, however, needs further study. Aurora kinase B (AURKB) functions as an important oncogene in various tumors; therefore, in the present study, we aimed to explore the mechanism by which AURKB affects ccRCC development. Methods We performed bioinformatics analysis, CCK-8 assay, RNA sequencing, RT-PCR and Western blot to analyze the function and mechanism of AURKB in ccRCC. Results TIMER2.0 showed that AURKB was overexpressed in Kidney Renal Clear Cell Carcinoma (KIRC), the UALCAN database showed the survival rate of KIRC patients with different expression levels of AURKB and different gender indicated in the same gender, high AURKB expression predicts lower survival rate. Silencing of AURKB expression inhibits the proliferation of ccRCC cells. RNA-seq data suggested that AURKB is involved in fatty acid metabolism. Silencing of AURKB inhibited the expression of fatty acid synthase (FASN). FASN is a key gene involved in fatty acid metabolism. TIMER2.0 showed that FASN is upregulated in KIRC. Silencing of FASN inhibited the proliferation of ccRCC cells. Conclusions AURKB induces the proliferation of ccRCC cells by regulating fatty acid metabolism. |
| format | Article |
| id | doaj-art-385734b5663849a0bd56966012619897 |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-385734b5663849a0bd569660126198972025-02-02T12:30:40ZengSpringerDiscover Oncology2730-60112025-01-0116111210.1007/s12672-024-01352-yAURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolismYang Yang0Dan Li1Zhigang Liu2Kai Zhou3Wenxing Li4Yanqi Yang5Ruifang Sun6Yulong Li7School of Public Health, Shaanxi University of Chinese MedicineDepartment of Cell Biology and Genetics, Medical College of Yan’an UniversityDepartment of Thoracic Surgery, Shaanxi Provincial Cancer Hospital, Xi’an Jiaotong UniversityDepartment of Surgical Oncology, The First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Surgical Oncology, The First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Pathology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science CenterDepartment of Pathology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science CenterDepartment of Gastroenterology, Shaanxi Provincial People’s HospitalAbstract Background Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer with a high metastatic rate and high mortality rate. The molecular mechanism of ccRCC development, however, needs further study. Aurora kinase B (AURKB) functions as an important oncogene in various tumors; therefore, in the present study, we aimed to explore the mechanism by which AURKB affects ccRCC development. Methods We performed bioinformatics analysis, CCK-8 assay, RNA sequencing, RT-PCR and Western blot to analyze the function and mechanism of AURKB in ccRCC. Results TIMER2.0 showed that AURKB was overexpressed in Kidney Renal Clear Cell Carcinoma (KIRC), the UALCAN database showed the survival rate of KIRC patients with different expression levels of AURKB and different gender indicated in the same gender, high AURKB expression predicts lower survival rate. Silencing of AURKB expression inhibits the proliferation of ccRCC cells. RNA-seq data suggested that AURKB is involved in fatty acid metabolism. Silencing of AURKB inhibited the expression of fatty acid synthase (FASN). FASN is a key gene involved in fatty acid metabolism. TIMER2.0 showed that FASN is upregulated in KIRC. Silencing of FASN inhibited the proliferation of ccRCC cells. Conclusions AURKB induces the proliferation of ccRCC cells by regulating fatty acid metabolism.https://doi.org/10.1007/s12672-024-01352-yccRCCAURKBProliferationFatty acidFASN |
| spellingShingle | Yang Yang Dan Li Zhigang Liu Kai Zhou Wenxing Li Yanqi Yang Ruifang Sun Yulong Li AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism Discover Oncology ccRCC AURKB Proliferation Fatty acid FASN |
| title | AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism |
| title_full | AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism |
| title_fullStr | AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism |
| title_full_unstemmed | AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism |
| title_short | AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism |
| title_sort | aurkb affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism |
| topic | ccRCC AURKB Proliferation Fatty acid FASN |
| url | https://doi.org/10.1007/s12672-024-01352-y |
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