Effect of Associative Learning on Memory Spine Formation in Mouse Barrel Cortex
Associative fear learning, in which stimulation of whiskers is paired with mild electric shock to the tail, modifies the barrel cortex, the functional representation of sensory receptors involved in the conditioning, by inducing formation of new inhibitory synapses on single-synapse spines of the co...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/9828517 |
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| author | Malgorzata Jasinska Ewa Siucinska Ewa Jasek Jan A. Litwin Elzbieta Pyza Malgorzata Kossut |
| author_facet | Malgorzata Jasinska Ewa Siucinska Ewa Jasek Jan A. Litwin Elzbieta Pyza Malgorzata Kossut |
| author_sort | Malgorzata Jasinska |
| collection | DOAJ |
| description | Associative fear learning, in which stimulation of whiskers is paired with mild electric shock to the tail, modifies the barrel cortex, the functional representation of sensory receptors involved in the conditioning, by inducing formation of new inhibitory synapses on single-synapse spines of the cognate barrel hollows and thus producing double-synapse spines. In the barrel cortex of conditioned, pseudoconditioned, and untreated mice, we analyzed the number and morphological features of dendritic spines at various maturation and stability levels: sER-free spines, spines containing smooth endoplasmic reticulum (sER), and spines containing spine apparatus. Using stereological analysis of serial sections examined by transmission electron microscopy, we found that the density of double-synapse spines containing spine apparatus was significantly increased in the conditioned mice. Learning also induced enhancement of the postsynaptic density area of inhibitory synapses as well as increase in the number of polyribosomes in such spines. In single-synapse spines, the effects of conditioning were less pronounced and included increase in the number of polyribosomes in sER-free spines. The results suggest that fear learning differentially affects single- and double-synapse spines in the barrel cortex: it promotes maturation and stabilization of double-synapse spines, which might possibly contribute to permanent memory formation, and upregulates protein synthesis in single-synapse spines. |
| format | Article |
| id | doaj-art-3844ec5833e140acab5605fc1b3e255e |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-3844ec5833e140acab5605fc1b3e255e2025-02-03T05:51:55ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/98285179828517Effect of Associative Learning on Memory Spine Formation in Mouse Barrel CortexMalgorzata Jasinska0Ewa Siucinska1Ewa Jasek2Jan A. Litwin3Elzbieta Pyza4Malgorzata Kossut5Department of Histology, Jagiellonian University Medical College, 7 Kopernika Street, 31-034 Krakow, PolandDepartment of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, PolandDepartment of Histology, Jagiellonian University Medical College, 7 Kopernika Street, 31-034 Krakow, PolandDepartment of Histology, Jagiellonian University Medical College, 7 Kopernika Street, 31-034 Krakow, PolandDepartment of Cell Biology and Imaging, Institute of Zoology, Jagiellonian University, 9 Gronostajowa Street, 30-387 Krakow, PolandDepartment of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, PolandAssociative fear learning, in which stimulation of whiskers is paired with mild electric shock to the tail, modifies the barrel cortex, the functional representation of sensory receptors involved in the conditioning, by inducing formation of new inhibitory synapses on single-synapse spines of the cognate barrel hollows and thus producing double-synapse spines. In the barrel cortex of conditioned, pseudoconditioned, and untreated mice, we analyzed the number and morphological features of dendritic spines at various maturation and stability levels: sER-free spines, spines containing smooth endoplasmic reticulum (sER), and spines containing spine apparatus. Using stereological analysis of serial sections examined by transmission electron microscopy, we found that the density of double-synapse spines containing spine apparatus was significantly increased in the conditioned mice. Learning also induced enhancement of the postsynaptic density area of inhibitory synapses as well as increase in the number of polyribosomes in such spines. In single-synapse spines, the effects of conditioning were less pronounced and included increase in the number of polyribosomes in sER-free spines. The results suggest that fear learning differentially affects single- and double-synapse spines in the barrel cortex: it promotes maturation and stabilization of double-synapse spines, which might possibly contribute to permanent memory formation, and upregulates protein synthesis in single-synapse spines.http://dx.doi.org/10.1155/2016/9828517 |
| spellingShingle | Malgorzata Jasinska Ewa Siucinska Ewa Jasek Jan A. Litwin Elzbieta Pyza Malgorzata Kossut Effect of Associative Learning on Memory Spine Formation in Mouse Barrel Cortex Neural Plasticity |
| title | Effect of Associative Learning on Memory Spine Formation in Mouse Barrel Cortex |
| title_full | Effect of Associative Learning on Memory Spine Formation in Mouse Barrel Cortex |
| title_fullStr | Effect of Associative Learning on Memory Spine Formation in Mouse Barrel Cortex |
| title_full_unstemmed | Effect of Associative Learning on Memory Spine Formation in Mouse Barrel Cortex |
| title_short | Effect of Associative Learning on Memory Spine Formation in Mouse Barrel Cortex |
| title_sort | effect of associative learning on memory spine formation in mouse barrel cortex |
| url | http://dx.doi.org/10.1155/2016/9828517 |
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