Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activity
Citrus is the world's largest fruit, developed a rich variety resources in a long history of cultivation. Flavonoid is a natural product found widely in citrus plants. In this study, the major flavonoids and relative activities of 9 citrus peels were investigated by using ultra-performance liqu...
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Elsevier
2024-12-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X2400248X |
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| author | Yulin ZHENG Yingxian WU Biying WANG Hong WANG Yuanhong ZHANG Wei WANG Limei YU |
| author_facet | Yulin ZHENG Yingxian WU Biying WANG Hong WANG Yuanhong ZHANG Wei WANG Limei YU |
| author_sort | Yulin ZHENG |
| collection | DOAJ |
| description | Citrus is the world's largest fruit, developed a rich variety resources in a long history of cultivation. Flavonoid is a natural product found widely in citrus plants. In this study, the major flavonoids and relative activities of 9 citrus peels were investigated by using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) analysis and antioxidant and anti-inflammatory assays in A549 cell. A total of 51 flavonoids were identified, comprising a variety of subclasses. Notably, Citrus Reticulata Blanco (MJ) and Bu Zhi Huo (CJ) demonstrated distinctive flavonoid profiles. Flavonoid extracts from MJ and Citrus maxima (HY) peels significantly reduced reactive oxygen species (ROS) generation and decreased the levels of cytokines (IL-8, TNF-α, MCP-1, and IL-6) in A549 cells. Furthermore, these extracts up-regulated the expression of Nrf2 and HO-1 and inhibited the phosphorylation of p65 and NF-κB activity. Gray correlation analysis identified (-)-vestitone, dimefline, eupafolin, and trans-cinnamaldehyde as strongly associated with inflammatory markers. The findings suggest that flavonoids extracted from citrus peels possess significant antioxidant and anti-inflammatory activities, highlighting their potential as natural therapeutic agents for combating inflammation. |
| format | Article |
| id | doaj-art-383485ad3d2e4f87b0b01339bf4fe9d9 |
| institution | OA Journals |
| issn | 2772-753X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Food Chemistry Advances |
| spelling | doaj-art-383485ad3d2e4f87b0b01339bf4fe9d92025-08-20T02:38:35ZengElsevierFood Chemistry Advances2772-753X2024-12-01510085310.1016/j.focha.2024.100853Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activityYulin ZHENG0Yingxian WU1Biying WANG2Hong WANG3Yuanhong ZHANG4Wei WANG5Limei YU6College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Food Sciences, South China Agricultural University, Guangzhou 510640, ChinaCollege of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; Corresponding authors.College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; Corresponding authors.Citrus is the world's largest fruit, developed a rich variety resources in a long history of cultivation. Flavonoid is a natural product found widely in citrus plants. In this study, the major flavonoids and relative activities of 9 citrus peels were investigated by using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) analysis and antioxidant and anti-inflammatory assays in A549 cell. A total of 51 flavonoids were identified, comprising a variety of subclasses. Notably, Citrus Reticulata Blanco (MJ) and Bu Zhi Huo (CJ) demonstrated distinctive flavonoid profiles. Flavonoid extracts from MJ and Citrus maxima (HY) peels significantly reduced reactive oxygen species (ROS) generation and decreased the levels of cytokines (IL-8, TNF-α, MCP-1, and IL-6) in A549 cells. Furthermore, these extracts up-regulated the expression of Nrf2 and HO-1 and inhibited the phosphorylation of p65 and NF-κB activity. Gray correlation analysis identified (-)-vestitone, dimefline, eupafolin, and trans-cinnamaldehyde as strongly associated with inflammatory markers. The findings suggest that flavonoids extracted from citrus peels possess significant antioxidant and anti-inflammatory activities, highlighting their potential as natural therapeutic agents for combating inflammation.http://www.sciencedirect.com/science/article/pii/S2772753X2400248XCitrus varietiesFlavonoid metabolitesIdentificationReactive oxygen species (ROS)Anti-inflammatory |
| spellingShingle | Yulin ZHENG Yingxian WU Biying WANG Hong WANG Yuanhong ZHANG Wei WANG Limei YU Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activity Food Chemistry Advances Citrus varieties Flavonoid metabolites Identification Reactive oxygen species (ROS) Anti-inflammatory |
| title | Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activity |
| title_full | Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activity |
| title_fullStr | Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activity |
| title_full_unstemmed | Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activity |
| title_short | Flavonoids analysis in citrus peels by UPLC-Q-TOF-MS/MS and its antioxidant and anti-inflammation activity |
| title_sort | flavonoids analysis in citrus peels by uplc q tof ms ms and its antioxidant and anti inflammation activity |
| topic | Citrus varieties Flavonoid metabolites Identification Reactive oxygen species (ROS) Anti-inflammatory |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X2400248X |
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