Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)

Selenium (Se) is an essential trace element critical for animal growth and immune function. This study investigated the dietary selenium requirement of juvenile large yellow croaker (<i>Larimichthys crocea</i>) through an 8-week feeding trial. Five experimental diets were formulated by s...

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Main Authors: Jinxing Xiao, Zhoudi Miao, Shiliang Dong, Kaiyang Wang, Fan Zhou, Zilong Li
Format: Article
Language:English
Published: MDPI AG 2025-08-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/15/2292
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author Jinxing Xiao
Zhoudi Miao
Shiliang Dong
Kaiyang Wang
Fan Zhou
Zilong Li
author_facet Jinxing Xiao
Zhoudi Miao
Shiliang Dong
Kaiyang Wang
Fan Zhou
Zilong Li
author_sort Jinxing Xiao
collection DOAJ
description Selenium (Se) is an essential trace element critical for animal growth and immune function. This study investigated the dietary selenium requirement of juvenile large yellow croaker (<i>Larimichthys crocea</i>) through an 8-week feeding trial. Five experimental diets were formulated by supplementing a basal diet with selenium polysaccharides (Se-PS) at 0, 20, 30, 40, and 50 mg/kg, resulting in analyzed Se concentrations of 0.35, 0.54, 0.71, 0.93, and 1.11 mg/kg, respectively. The results demonstrated that growth performance and feed efficiency improved with increasing dietary selenium, peaking at 0.93 mg/kg before declining at higher levels. Antioxidant enzyme activities—superoxide dismutase (SOD) and catalase (CAT)—in serum and liver tissues exhibited a dose-dependent increase, reaching maximal levels at 1.11 mg/kg. Conversely, malondialdehyde (MDA), a marker of oxidative stress, progressively decreased in both serum and liver, attaining its lowest concentration at 1.11 mg/kg, though this did not differ significantly from the 0.93 mg/kg group (<i>p</i> = 0.056). Tissue selenium accumulation was highest at these optimal dietary levels. Based on the growth performance, oxidative stress response, and tissue selenium retention, the recommended dietary selenium requirement for juvenile large yellow croaker is 0.93 mg/kg. These findings highlight the importance of optimal Se supplementation in aquafeeds to enhance growth and physiological health in farmed fish.
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spelling doaj-art-3832c802bd66469cbb9fd2fb89132c0a2025-08-20T03:02:55ZengMDPI AGAnimals2076-26152025-08-011515229210.3390/ani15152292Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)Jinxing Xiao0Zhoudi Miao1Shiliang Dong2Kaiyang Wang3Fan Zhou4Zilong Li5Ocean College, Zhejiang University, Zhoushan 316000, ChinaOcean Research Center of Zhoushan, Zhejiang University, Zhoushan 316000, ChinaOcean Research Center of Zhoushan, Zhejiang University, Zhoushan 316000, ChinaOcean Research Center of Zhoushan, Zhejiang University, Zhoushan 316000, ChinaZhejiang Fisheries Technical Extension Station, Hangzhou 311000, ChinaOcean College, Zhejiang University, Zhoushan 316000, ChinaSelenium (Se) is an essential trace element critical for animal growth and immune function. This study investigated the dietary selenium requirement of juvenile large yellow croaker (<i>Larimichthys crocea</i>) through an 8-week feeding trial. Five experimental diets were formulated by supplementing a basal diet with selenium polysaccharides (Se-PS) at 0, 20, 30, 40, and 50 mg/kg, resulting in analyzed Se concentrations of 0.35, 0.54, 0.71, 0.93, and 1.11 mg/kg, respectively. The results demonstrated that growth performance and feed efficiency improved with increasing dietary selenium, peaking at 0.93 mg/kg before declining at higher levels. Antioxidant enzyme activities—superoxide dismutase (SOD) and catalase (CAT)—in serum and liver tissues exhibited a dose-dependent increase, reaching maximal levels at 1.11 mg/kg. Conversely, malondialdehyde (MDA), a marker of oxidative stress, progressively decreased in both serum and liver, attaining its lowest concentration at 1.11 mg/kg, though this did not differ significantly from the 0.93 mg/kg group (<i>p</i> = 0.056). Tissue selenium accumulation was highest at these optimal dietary levels. Based on the growth performance, oxidative stress response, and tissue selenium retention, the recommended dietary selenium requirement for juvenile large yellow croaker is 0.93 mg/kg. These findings highlight the importance of optimal Se supplementation in aquafeeds to enhance growth and physiological health in farmed fish.https://www.mdpi.com/2076-2615/15/15/2292<i>Larimichthys crocea</i>selenized polysaccharidesgrowth performanceoxidative stresstissue selenium content
spellingShingle Jinxing Xiao
Zhoudi Miao
Shiliang Dong
Kaiyang Wang
Fan Zhou
Zilong Li
Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)
Animals
<i>Larimichthys crocea</i>
selenized polysaccharides
growth performance
oxidative stress
tissue selenium content
title Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)
title_full Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)
title_fullStr Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)
title_full_unstemmed Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)
title_short Optimizing Selenium Polysaccharide Supplementation: Impacts on Growth, Oxidative Stress, and Tissue Selenium in Juvenile Large Yellow Croaker (<i>Larimichthys crocea</i>)
title_sort optimizing selenium polysaccharide supplementation impacts on growth oxidative stress and tissue selenium in juvenile large yellow croaker i larimichthys crocea i
topic <i>Larimichthys crocea</i>
selenized polysaccharides
growth performance
oxidative stress
tissue selenium content
url https://www.mdpi.com/2076-2615/15/15/2292
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