Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction
Persistent polyclonal B cell lymphocytosis (PPBL) is a rare disorder, diagnosed primarily in adult female smokers and characterized by an expansion of CD19+CD27+IgM+ memory B cells, by the presence of binucleated lymphocytes, and by a moderate elevation of serum IgM. The clinical course is usually b...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Advances in Hematology |
| Online Access: | http://dx.doi.org/10.1155/2014/854124 |
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| author | Emmanuelle Dugas-Bourdages Sonia Néron Annie Roy André Darveau Robert Delage |
| author_facet | Emmanuelle Dugas-Bourdages Sonia Néron Annie Roy André Darveau Robert Delage |
| author_sort | Emmanuelle Dugas-Bourdages |
| collection | DOAJ |
| description | Persistent polyclonal B cell lymphocytosis (PPBL) is a rare disorder, diagnosed primarily in adult female smokers and characterized by an expansion of CD19+CD27+IgM+ memory B cells, by the presence of binucleated lymphocytes, and by a moderate elevation of serum IgM. The clinical course is usually benign, but it is not known whether or not PPBL might be part of a process leading to the emergence of a malignant proliferative disorder. In this study we sought to investigate the functional response of B cells from patients with PPBL by use of an optimal memory B cell culture model based on the CD40-CD154 interaction. We found that the proliferation of PPBL B cells was almost as important as that of B cells from normal controls, resulting in high immunoglobulin secretion with in vitro isotypic switching. We conclude that the CD40-CD154 activation pathway is functional in the memory B cell population of PPBL patients, suggesting that the disorder may be due to either a dysfunction of other cells in the microenvironment or a possible defect in another B cell activation pathway. |
| format | Article |
| id | doaj-art-382a38688ba0448dbfa2c51f883beea6 |
| institution | Kabale University |
| issn | 1687-9104 1687-9112 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Hematology |
| spelling | doaj-art-382a38688ba0448dbfa2c51f883beea62025-02-03T05:52:48ZengWileyAdvances in Hematology1687-91041687-91122014-01-01201410.1155/2014/854124854124Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 InteractionEmmanuelle Dugas-Bourdages0Sonia Néron1Annie Roy2André Darveau3Robert Delage4Centre Universitaire d’Hématologie et d’Oncologie de Québec, CHU de Québec, Hôpital de l’Enfant-Jésus, 1401 18ième rue, Québec, QC, G1J 1Z4, CanadaHéma-Québec, Recherche et Développement, 1070 avenue des Sciences-de-la-Vie, Québec, QC, G1V 5C3, CanadaHéma-Québec, Recherche et Développement, 1070 avenue des Sciences-de-la-Vie, Québec, QC, G1V 5C3, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Pavillon Alexandre-Vachon, 1045 avenue de la Médecine, Bureau 3428, Université Laval, Québec, QC, G1V 0A6, CanadaCentre Universitaire d’Hématologie et d’Oncologie de Québec, CHU de Québec, Hôpital de l’Enfant-Jésus, 1401 18ième rue, Québec, QC, G1J 1Z4, CanadaPersistent polyclonal B cell lymphocytosis (PPBL) is a rare disorder, diagnosed primarily in adult female smokers and characterized by an expansion of CD19+CD27+IgM+ memory B cells, by the presence of binucleated lymphocytes, and by a moderate elevation of serum IgM. The clinical course is usually benign, but it is not known whether or not PPBL might be part of a process leading to the emergence of a malignant proliferative disorder. In this study we sought to investigate the functional response of B cells from patients with PPBL by use of an optimal memory B cell culture model based on the CD40-CD154 interaction. We found that the proliferation of PPBL B cells was almost as important as that of B cells from normal controls, resulting in high immunoglobulin secretion with in vitro isotypic switching. We conclude that the CD40-CD154 activation pathway is functional in the memory B cell population of PPBL patients, suggesting that the disorder may be due to either a dysfunction of other cells in the microenvironment or a possible defect in another B cell activation pathway.http://dx.doi.org/10.1155/2014/854124 |
| spellingShingle | Emmanuelle Dugas-Bourdages Sonia Néron Annie Roy André Darveau Robert Delage Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction Advances in Hematology |
| title | Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction |
| title_full | Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction |
| title_fullStr | Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction |
| title_full_unstemmed | Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction |
| title_short | Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction |
| title_sort | persistent polyclonal b cell lymphocytosis b cells can be activated through cd40 cd154 interaction |
| url | http://dx.doi.org/10.1155/2014/854124 |
| work_keys_str_mv | AT emmanuelledugasbourdages persistentpolyclonalbcelllymphocytosisbcellscanbeactivatedthroughcd40cd154interaction AT sonianeron persistentpolyclonalbcelllymphocytosisbcellscanbeactivatedthroughcd40cd154interaction AT annieroy persistentpolyclonalbcelllymphocytosisbcellscanbeactivatedthroughcd40cd154interaction AT andredarveau persistentpolyclonalbcelllymphocytosisbcellscanbeactivatedthroughcd40cd154interaction AT robertdelage persistentpolyclonalbcelllymphocytosisbcellscanbeactivatedthroughcd40cd154interaction |