Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles.
<h4>Background</h4>Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped.<h4>Methodology/principal findings<...
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| Format: | Article |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0137820 |
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| author | Martin Převorovský Martina Oravcová Jarmila Tvarůžková Róbert Zach Petr Folk František Půta Jürg Bähler |
| author_facet | Martin Převorovský Martina Oravcová Jarmila Tvarůžková Róbert Zach Petr Folk František Půta Jürg Bähler |
| author_sort | Martin Převorovský |
| collection | DOAJ |
| description | <h4>Background</h4>Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped.<h4>Methodology/principal findings</h4>Using a combination of transcriptome profiling under various conditions and genome-wide analysis of CSL-DNA interactions, we identify genes regulated directly and indirectly by CSL proteins in fission yeast. We show that the expression of stress-response genes and genes that are expressed periodically during the cell cycle is deregulated upon genetic manipulation of cbf11 and/or cbf12. Accordingly, the coordination of mitosis and cytokinesis is perturbed in cells with genetically manipulated CSL protein levels, together with other specific defects in cell-cycle progression. Cbf11 activity is nutrient-dependent and Δcbf11-associated defects are mitigated by inactivation of the protein kinase A (Pka1) and stress-activated MAP kinase (Sty1p38) pathways. Furthermore, Cbf11 directly regulates a set of lipid metabolism genes and Δcbf11 cells feature a stark decrease in the number of storage lipid droplets.<h4>Conclusions/significance</h4>Our results provide a framework for a more detailed understanding of the role of CSL proteins in the regulation of cell-cycle progression in fission yeast. |
| format | Article |
| id | doaj-art-381a333f68f147c78c60bbbbec7d192e |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-381a333f68f147c78c60bbbbec7d192e2025-08-20T02:22:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013782010.1371/journal.pone.0137820Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles.Martin PřevorovskýMartina OravcováJarmila TvarůžkováRóbert ZachPetr FolkFrantišek PůtaJürg Bähler<h4>Background</h4>Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped.<h4>Methodology/principal findings</h4>Using a combination of transcriptome profiling under various conditions and genome-wide analysis of CSL-DNA interactions, we identify genes regulated directly and indirectly by CSL proteins in fission yeast. We show that the expression of stress-response genes and genes that are expressed periodically during the cell cycle is deregulated upon genetic manipulation of cbf11 and/or cbf12. Accordingly, the coordination of mitosis and cytokinesis is perturbed in cells with genetically manipulated CSL protein levels, together with other specific defects in cell-cycle progression. Cbf11 activity is nutrient-dependent and Δcbf11-associated defects are mitigated by inactivation of the protein kinase A (Pka1) and stress-activated MAP kinase (Sty1p38) pathways. Furthermore, Cbf11 directly regulates a set of lipid metabolism genes and Δcbf11 cells feature a stark decrease in the number of storage lipid droplets.<h4>Conclusions/significance</h4>Our results provide a framework for a more detailed understanding of the role of CSL proteins in the regulation of cell-cycle progression in fission yeast.https://doi.org/10.1371/journal.pone.0137820 |
| spellingShingle | Martin Převorovský Martina Oravcová Jarmila Tvarůžková Róbert Zach Petr Folk František Půta Jürg Bähler Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles. PLoS ONE |
| title | Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles. |
| title_full | Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles. |
| title_fullStr | Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles. |
| title_full_unstemmed | Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles. |
| title_short | Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles. |
| title_sort | fission yeast csl transcription factors mapping their target genes and biological roles |
| url | https://doi.org/10.1371/journal.pone.0137820 |
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