Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India
Objective. The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India. Method. Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/9042125 |
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| author | Rucha Patki Sunil Lilani Dhaneshwar Lanjewar |
| author_facet | Rucha Patki Sunil Lilani Dhaneshwar Lanjewar |
| author_sort | Rucha Patki |
| collection | DOAJ |
| description | Objective. The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India. Method. Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera (with 0.9% normal saline) of blood donors, with final dilution ranging from 1 : 40 to 1 : 320. Results. Out of 400 individuals providing samples, 78 (19.5%) individuals showed antibody titres ≥ 1 : 40 for at least one antigen and 322 (80.5%) showed no agglutination. The baseline antibody titres against O antigen and H antigen of Salmonella enterica serotype Typhi were found to be 1 : 40 and 1 : 80, respectively. Similarly, the baseline antibody titres for the H antigen of Salmonella enterica serotypes Paratyphi A and Paratyphi B were found to be 1 : 40 and 1 : 80, respectively. Conclusion. Thus, it was noted that the diagnostically significant cutoff of antibody titre from acute phase sample was ≥ 1 : 80 for S. Typhi O antigen and titre of ≥ 1 : 160 for both S. Typhi H antigen and S. Paratyphi BH antigen. Antibody titre of ≥ 1 : 80 can be considered significant for S. Paratyphi AH antigen. |
| format | Article |
| id | doaj-art-3810fac6637c47ef95e6c0eda7d75558 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-3810fac6637c47ef95e6c0eda7d755582025-02-03T05:52:49ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/90421259042125Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, IndiaRucha Patki0Sunil Lilani1Dhaneshwar Lanjewar2Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai, Maharashtra, IndiaShri Bhausaheb Hire Government Medical College and Hospital, Dhule, IndiaDepartment of Pathology, Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai, Maharashtra, IndiaObjective. The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India. Method. Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera (with 0.9% normal saline) of blood donors, with final dilution ranging from 1 : 40 to 1 : 320. Results. Out of 400 individuals providing samples, 78 (19.5%) individuals showed antibody titres ≥ 1 : 40 for at least one antigen and 322 (80.5%) showed no agglutination. The baseline antibody titres against O antigen and H antigen of Salmonella enterica serotype Typhi were found to be 1 : 40 and 1 : 80, respectively. Similarly, the baseline antibody titres for the H antigen of Salmonella enterica serotypes Paratyphi A and Paratyphi B were found to be 1 : 40 and 1 : 80, respectively. Conclusion. Thus, it was noted that the diagnostically significant cutoff of antibody titre from acute phase sample was ≥ 1 : 80 for S. Typhi O antigen and titre of ≥ 1 : 160 for both S. Typhi H antigen and S. Paratyphi BH antigen. Antibody titre of ≥ 1 : 80 can be considered significant for S. Paratyphi AH antigen.http://dx.doi.org/10.1155/2017/9042125 |
| spellingShingle | Rucha Patki Sunil Lilani Dhaneshwar Lanjewar Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India International Journal of Microbiology |
| title | Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India |
| title_full | Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India |
| title_fullStr | Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India |
| title_full_unstemmed | Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India |
| title_short | Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India |
| title_sort | baseline antibody titre against salmonella enterica in healthy population of mumbai maharashtra india |
| url | http://dx.doi.org/10.1155/2017/9042125 |
| work_keys_str_mv | AT ruchapatki baselineantibodytitreagainstsalmonellaentericainhealthypopulationofmumbaimaharashtraindia AT sunillilani baselineantibodytitreagainstsalmonellaentericainhealthypopulationofmumbaimaharashtraindia AT dhaneshwarlanjewar baselineantibodytitreagainstsalmonellaentericainhealthypopulationofmumbaimaharashtraindia |