Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SN

Background and Aim: Feed additives composed of natural minerals and probiotics are increasingly explored in aquaculture to improve fish health and meat quality while reducing antibiotic dependency. Zeolite and vermiculite, due to their adsorptive and ion-exchange properties, enhance mineral bioavail...

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Main Authors: Nurzhan Sarsembayeva, Gulmariya Ikramzhan, Tolkyn Abdigaliyeva, Zhumagul Kirkimbaeva, Birzhan Biyashev, Saule Sherimova, Primkul Ibragimov
Format: Article
Language:English
Published: Veterinary World 2025-06-01
Series:Veterinary World
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Online Access:https://www.veterinaryworld.org/Vol.18/June-2025/11.pdf
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author Nurzhan Sarsembayeva
Gulmariya Ikramzhan
Tolkyn Abdigaliyeva
Zhumagul Kirkimbaeva
Birzhan Biyashev
Saule Sherimova
Primkul Ibragimov
author_facet Nurzhan Sarsembayeva
Gulmariya Ikramzhan
Tolkyn Abdigaliyeva
Zhumagul Kirkimbaeva
Birzhan Biyashev
Saule Sherimova
Primkul Ibragimov
author_sort Nurzhan Sarsembayeva
collection DOAJ
description Background and Aim: Feed additives composed of natural minerals and probiotics are increasingly explored in aquaculture to improve fish health and meat quality while reducing antibiotic dependency. Zeolite and vermiculite, due to their adsorptive and ion-exchange properties, enhance mineral bioavailability, whereas probiotics, such as Escherichia coli 39-SN improve digestive function and nutrient assimilation. This study aimed to evaluate the effects of dietary inclusion of zeolite or vermiculite in combination with E. coli 39-SN on the chemical, mineral, and amino acid composition of African sharptooth catfish (Clarias gariepinus). Materials and Methods: A total of 150 catfish were divided into three groups: A control group fed a standard diet, and two experimental groups receiving diets supplemented with either 5% zeolite or 5% vermiculite, each combined with 0.1% E. coli 39-SN. The trial lasted 87 days under controlled aquaculture conditions. Post-trial, muscle tissues were analyzed for moisture, fat, protein, ash content, energy value, mineral content (e.g., iron, zinc, and magnesium), and amino acid profiles using standardized laboratory methods. Results: Fish in the experimental groups exhibited improved nutritional profiles compared to the control. Notably, the zeolite + probiotic group showed the highest total amino acid content (34.63%) and significant increases in essential amino acids, such as leucine and phenylalanine. Vermiculite + probiotic supplementation yielded the highest fat content (0.69 g/100 g) and increased concentrations of iron and zinc. Both experimental groups demonstrated improved energy values, elevated mineral levels, and enhanced biological value of the muscle tissue. No signs of stress or cannibalism were observed, indicating good tolerance to the diets. Conclusion: The synergistic use of natural minerals and E. coli 39-SN significantly enhanced the chemical and nutritional quality of C. gariepinus muscle tissue. These findings suggest that such dietary strategies offer a promising alternative to antibiotics and can enhance the commercial value of aquaculture products. Future studies should explore dose optimization, long-term health effects, and the immunomodulatory potential of E. coli 39-SN in fish.
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spelling doaj-art-3808414bc2b24a3d9ff4bef3bd7ddd992025-08-20T03:47:17ZengVeterinary WorldVeterinary World0972-89882231-09162025-06-011861517152610.14202/vetworld.2025.1517-1526Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SNNurzhan Sarsembayeva0https://orcid.org/0000-0002-3501-3720Gulmariya Ikramzhan1https://orcid.org/0009-0006-6761-2337Tolkyn Abdigaliyeva2https://orcid.org/0000-0002-1404-8852Zhumagul Kirkimbaeva3https://orcid.org/0000-0003-3873-9049Birzhan Biyashev4https://orcid.org/0009-0008-4193-3567Saule Sherimova5https://orcid.org/0000-0002-0436-5467Primkul Ibragimov6https://orcid.org/0000-0002-0084-9464Department of Veterinary Sanitary Examination and Hygiene, Kazakh National Agrarian Research University, Almaty, Kazakhstan.Department of Veterinary Sanitary Examination and Hygiene, Kazakh National Agrarian Research University, Almaty, Kazakhstan.Department of Food Biotechnology, Almaty Technological University, Almaty, Kazakhstan.Department of Microbiology, Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan.Department of Microbiology, Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan.Department of Veterinary medicine, M. Auezov South Kazakhstan university, Shymkent, Kazakhstan.Department of Biological Safety, Kazakh National Agrarian Research University, Almaty, Kazakhstan.Background and Aim: Feed additives composed of natural minerals and probiotics are increasingly explored in aquaculture to improve fish health and meat quality while reducing antibiotic dependency. Zeolite and vermiculite, due to their adsorptive and ion-exchange properties, enhance mineral bioavailability, whereas probiotics, such as Escherichia coli 39-SN improve digestive function and nutrient assimilation. This study aimed to evaluate the effects of dietary inclusion of zeolite or vermiculite in combination with E. coli 39-SN on the chemical, mineral, and amino acid composition of African sharptooth catfish (Clarias gariepinus). Materials and Methods: A total of 150 catfish were divided into three groups: A control group fed a standard diet, and two experimental groups receiving diets supplemented with either 5% zeolite or 5% vermiculite, each combined with 0.1% E. coli 39-SN. The trial lasted 87 days under controlled aquaculture conditions. Post-trial, muscle tissues were analyzed for moisture, fat, protein, ash content, energy value, mineral content (e.g., iron, zinc, and magnesium), and amino acid profiles using standardized laboratory methods. Results: Fish in the experimental groups exhibited improved nutritional profiles compared to the control. Notably, the zeolite + probiotic group showed the highest total amino acid content (34.63%) and significant increases in essential amino acids, such as leucine and phenylalanine. Vermiculite + probiotic supplementation yielded the highest fat content (0.69 g/100 g) and increased concentrations of iron and zinc. Both experimental groups demonstrated improved energy values, elevated mineral levels, and enhanced biological value of the muscle tissue. No signs of stress or cannibalism were observed, indicating good tolerance to the diets. Conclusion: The synergistic use of natural minerals and E. coli 39-SN significantly enhanced the chemical and nutritional quality of C. gariepinus muscle tissue. These findings suggest that such dietary strategies offer a promising alternative to antibiotics and can enhance the commercial value of aquaculture products. Future studies should explore dose optimization, long-term health effects, and the immunomodulatory potential of E. coli 39-SN in fish.https://www.veterinaryworld.org/Vol.18/June-2025/11.pdfamino acid profileaquacultureclarias gariepinusescherichia coli 39-snfeed additivesnutritional enhancementvermiculitezeolite
spellingShingle Nurzhan Sarsembayeva
Gulmariya Ikramzhan
Tolkyn Abdigaliyeva
Zhumagul Kirkimbaeva
Birzhan Biyashev
Saule Sherimova
Primkul Ibragimov
Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SN
Veterinary World
amino acid profile
aquaculture
clarias gariepinus
escherichia coli 39-sn
feed additives
nutritional enhancement
vermiculite
zeolite
title Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SN
title_full Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SN
title_fullStr Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SN
title_full_unstemmed Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SN
title_short Enhancing the nutritional profile of African sharptooth catfish (Clarias gariepinus) through dietary supplementation with natural minerals and probiotic Escherichia coli 39-SN
title_sort enhancing the nutritional profile of african sharptooth catfish clarias gariepinus through dietary supplementation with natural minerals and probiotic escherichia coli 39 sn
topic amino acid profile
aquaculture
clarias gariepinus
escherichia coli 39-sn
feed additives
nutritional enhancement
vermiculite
zeolite
url https://www.veterinaryworld.org/Vol.18/June-2025/11.pdf
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