<i>FoxO1</i> Mediated by H3K27me3 Inhibits Porcine Follicular Development by Regulating the Transcription of <i>CYP1A1</i>
It is well known that the function of granulosa cells (GCs) is closely related to follicular development, and <i>FoxO1</i> and histone methylation have been implicated in follicular development. However, the specific mechanisms by which <i>FoxO1</i> and histone methylation re...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/23/3514 |
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| Summary: | It is well known that the function of granulosa cells (GCs) is closely related to follicular development, and <i>FoxO1</i> and histone methylation have been implicated in follicular development. However, the specific mechanisms by which <i>FoxO1</i> and histone methylation regulate follicular development are still largely unknown. To explore the specific mechanism of <i>FoxO1</i> in regulating follicular development, in this study, we showed that the expression of <i>FoxO1</i> in immature ovaries and small follicles was significantly higher than in mature ovaries and large follicles of sows, respectively. <i>FoxO1</i> was found to inhibit the secretion of testosterone and proliferation of porcine GCs and promote the secretion of progesterone and apoptosis of porcine GCs. Furthermore, H3K27me3, as a transcriptional inhibitor, can inhibit the transcription of <i>FoxO1</i>. <i>FoxO1</i> could promote the transcription of <i>CYP1A1</i>, and <i>CYP1A1</i> was found to inhibit the proliferation and facilitate the ferroptosis of porcine GCs. Collectively, our results revealed that the H3K27me3-<i>FoxO1</i>-<i>CYP1A1</i> pathway might participate in follicular development, and these findings could provide potential targets for improving follicular development in sows. |
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| ISSN: | 2076-2615 |