Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern China
Avian leukosis virus (ALV) is a dangerous pathogen in poultry, causing high mortality and decreased fertility due to tumor formation. Generally, the ALV subgroup E (ALV-E) strains are typically low in pathogenicity and tumorigenicity. In this study, we isolated and identified a ALV strain JS1208 fro...
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Elsevier
2025-05-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125002925 |
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| author | Guige Xu Lei Bei Mingrui Zhao Hui Yan Huajie Zhang Shijin Jiang Ruihua Zhang |
| author_facet | Guige Xu Lei Bei Mingrui Zhao Hui Yan Huajie Zhang Shijin Jiang Ruihua Zhang |
| author_sort | Guige Xu |
| collection | DOAJ |
| description | Avian leukosis virus (ALV) is a dangerous pathogen in poultry, causing high mortality and decreased fertility due to tumor formation. Generally, the ALV subgroup E (ALV-E) strains are typically low in pathogenicity and tumorigenicity. In this study, we isolated and identified a ALV strain JS1208 from an AL-like tumor case in a commercial Hy-Line Brown layer flock in China. Immunofluorescence assays and transmission electron microscopy revealed that the JS1208 strain replicated in chick embryo fibroblasts (CEF). Complete genome sequencing and analysis identified JS1208 as a recombinant strain, with a major parental backbone from ALV subgroup K (ALV-K) and a minor contribution from ALV-E. Infection of specific-pathogen-free chickens with JS1208 induced typical clinical signs and tumor-like lesions similar to those observed in the original infected flock. To our best knowledge, this is the first report of a recombinant ALV-E strain containing an ALV-K backbone and an ALV-E gp85 gene, resulting in a novel circulating ALV-E variant. These findings will contribute to a deeper understanding of ALV epidemiology. |
| format | Article |
| id | doaj-art-37fccacd75ef49a999018ef60fd38510 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-37fccacd75ef49a999018ef60fd385102025-08-20T01:51:41ZengElsevierPoultry Science0032-57912025-05-01104510505310.1016/j.psj.2025.105053Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern ChinaGuige Xu0Lei Bei1Mingrui Zhao2Hui Yan3Huajie Zhang4Shijin Jiang5Ruihua Zhang6Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian 271017, PR China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, PR ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian 271017, PR China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, PR ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian 271017, PR China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, PR ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian 271017, PR China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, PR ChinaWeihai Animal Disease Prevention and Control Center, Shandong Weihai 264200, PR ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian 271017, PR China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, PR ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian 271017, PR China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, PR China; Correspondence author at. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271017, PR China.Avian leukosis virus (ALV) is a dangerous pathogen in poultry, causing high mortality and decreased fertility due to tumor formation. Generally, the ALV subgroup E (ALV-E) strains are typically low in pathogenicity and tumorigenicity. In this study, we isolated and identified a ALV strain JS1208 from an AL-like tumor case in a commercial Hy-Line Brown layer flock in China. Immunofluorescence assays and transmission electron microscopy revealed that the JS1208 strain replicated in chick embryo fibroblasts (CEF). Complete genome sequencing and analysis identified JS1208 as a recombinant strain, with a major parental backbone from ALV subgroup K (ALV-K) and a minor contribution from ALV-E. Infection of specific-pathogen-free chickens with JS1208 induced typical clinical signs and tumor-like lesions similar to those observed in the original infected flock. To our best knowledge, this is the first report of a recombinant ALV-E strain containing an ALV-K backbone and an ALV-E gp85 gene, resulting in a novel circulating ALV-E variant. These findings will contribute to a deeper understanding of ALV epidemiology.http://www.sciencedirect.com/science/article/pii/S0032579125002925Avian leukosis virusPathogenicityPhylogenetic analysisRecombinant analysis |
| spellingShingle | Guige Xu Lei Bei Mingrui Zhao Hui Yan Huajie Zhang Shijin Jiang Ruihua Zhang Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern China Poultry Science Avian leukosis virus Pathogenicity Phylogenetic analysis Recombinant analysis |
| title | Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern China |
| title_full | Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern China |
| title_fullStr | Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern China |
| title_full_unstemmed | Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern China |
| title_short | Isolation and characterization of a recombinant avian leukosis virus subgroup E from a commercial layer farm in Eastern China |
| title_sort | isolation and characterization of a recombinant avian leukosis virus subgroup e from a commercial layer farm in eastern china |
| topic | Avian leukosis virus Pathogenicity Phylogenetic analysis Recombinant analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125002925 |
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