Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs
Aim: siRNA can silence targeted genes with lesser toxicity than therapeutic drugs. Therefore, this study aims to investigate new approaches to treat pancreatic cancer (PC) using combinations of siRNA and gemcitabine. Methods: Three genes, ANGTPL4, Notch1 and NF-κβ1, were silenced using siRNA, and th...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Future Science OA |
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| Online Access: | https://www.tandfonline.com/doi/10.2144/fsoa-2023-0145 |
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| author | Abdulfattah Al-Kadash Walhan Alshaer Ismail Sami Mahmoud Suha Wehaibi Malek Zihlif |
| author_facet | Abdulfattah Al-Kadash Walhan Alshaer Ismail Sami Mahmoud Suha Wehaibi Malek Zihlif |
| author_sort | Abdulfattah Al-Kadash |
| collection | DOAJ |
| description | Aim: siRNA can silence targeted genes with lesser toxicity than therapeutic drugs. Therefore, this study aims to investigate new approaches to treat pancreatic cancer (PC) using combinations of siRNA and gemcitabine. Methods: Three genes, ANGTPL4, Notch1 and NF-κβ1, were silenced using siRNA, and their anti-proliferative effects were studied in combination with gemcitabine on pancreatic cancer cell line (PANC-1) using MTT viability assay. Results: Our results showed a significant reduction in PANC-1 cells growth upon treating cells with gemcitabine and single and combinations of siRNA sequences specific for ANGTPL4, Notch1 and NF-κβ1 genes. Conclusion: Co-transfection of gemcitabine-treated PANC-1 cells with ANGPTL4, Notch1 and NF-κβsiRNAs enhances the chemosensitivity of PANC-1 cells to gemcitabine can be a promising therapeutic approach. |
| format | Article |
| id | doaj-art-37fca30f7aa44cfbb9ccfc1e71f01738 |
| institution | Kabale University |
| issn | 2056-5623 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Future Science OA |
| spelling | doaj-art-37fca30f7aa44cfbb9ccfc1e71f017382025-08-20T03:50:38ZengTaylor & Francis GroupFuture Science OA2056-56232024-12-0110110.2144/fsoa-2023-0145Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAsAbdulfattah Al-Kadash0Walhan Alshaer1Ismail Sami Mahmoud2Suha Wehaibi3Malek Zihlif4Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman, 11942, JordanCell Therapy Center, The University of Jordan, Amman, 11942, JordanDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, The Hashemite University, Zarqa, 13133, JordanCell Therapy Center, The University of Jordan, Amman, 11942, JordanDepartment of Pharmacology, Faculty of Medicine, The University of Jordan, Amman, 11942, JordanAim: siRNA can silence targeted genes with lesser toxicity than therapeutic drugs. Therefore, this study aims to investigate new approaches to treat pancreatic cancer (PC) using combinations of siRNA and gemcitabine. Methods: Three genes, ANGTPL4, Notch1 and NF-κβ1, were silenced using siRNA, and their anti-proliferative effects were studied in combination with gemcitabine on pancreatic cancer cell line (PANC-1) using MTT viability assay. Results: Our results showed a significant reduction in PANC-1 cells growth upon treating cells with gemcitabine and single and combinations of siRNA sequences specific for ANGTPL4, Notch1 and NF-κβ1 genes. Conclusion: Co-transfection of gemcitabine-treated PANC-1 cells with ANGPTL4, Notch1 and NF-κβsiRNAs enhances the chemosensitivity of PANC-1 cells to gemcitabine can be a promising therapeutic approach.https://www.tandfonline.com/doi/10.2144/fsoa-2023-0145ANGPTL4gemcitabineNF-κβ1Notch1pancreatic cancersiRNA |
| spellingShingle | Abdulfattah Al-Kadash Walhan Alshaer Ismail Sami Mahmoud Suha Wehaibi Malek Zihlif Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs Future Science OA ANGPTL4 gemcitabine NF-κβ1 Notch1 pancreatic cancer siRNA |
| title | Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs |
| title_full | Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs |
| title_fullStr | Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs |
| title_full_unstemmed | Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs |
| title_short | Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs |
| title_sort | enhancing chemosensitivity of panc1 pancreatic cancer cells to gemcitabine using angtpl4 notch1 and nf κβ1 sirnas |
| topic | ANGPTL4 gemcitabine NF-κβ1 Notch1 pancreatic cancer siRNA |
| url | https://www.tandfonline.com/doi/10.2144/fsoa-2023-0145 |
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