Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structure

Abstract Background Intensive monoculture poses a serious threat to agricultural sustainable development due to the phenomenon of continuous cropping obstacles. Although organic amendment has been considered an efficient and environmentally friendly solution to mitigate this tough issue, the associa...

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Main Authors: Dan Chen, Yujie Zhou, Gang Wang, Kuai Dai, Jiangzhou Li, Xueru Song, Yongxian Xu, Yonghe Cui, Xiaoe Yang
Format: Article
Language:English
Published: BMC 2025-03-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-025-06266-7
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author Dan Chen
Yujie Zhou
Gang Wang
Kuai Dai
Jiangzhou Li
Xueru Song
Yongxian Xu
Yonghe Cui
Xiaoe Yang
author_facet Dan Chen
Yujie Zhou
Gang Wang
Kuai Dai
Jiangzhou Li
Xueru Song
Yongxian Xu
Yonghe Cui
Xiaoe Yang
author_sort Dan Chen
collection DOAJ
description Abstract Background Intensive monoculture poses a serious threat to agricultural sustainable development due to the phenomenon of continuous cropping obstacles. Although organic amendment has been considered an efficient and environmentally friendly solution to mitigate this tough issue, the associated mechanisms remain poorly understood. Here, a two-year field experiment was conducted with the application of four fertilizers, wood, rice straw, compound biochar-based organic fertilizers (WBF, RBF, CBF) and chemical fertilizer (CF) under tobacco rotation with broad bean and oilseed rape, respectively. This work aims to determine how BFs application alleviates tobacco CCO and to further reveal the underlying action mechanisms primarily focusing on the change of soil micro-ecology environments. Results The results depicted that BFs addition decreased tobacco morbidity (by 15.7–85.0%), heavy metals (Cd, V, Cu, Zn) contents in tobacco, and improved tobacco leaf production yield (by 4.5–20.5%), economic value (by 14.6–34.4%) and chemical quality compared with CF. Rhizosphere soil chemical properties and the structure and diversity of microbial communities were enhanced under BFs treatments, reflecting in the growth of bacterial OTUs number, microbial alpha-diversity, the abundances of some beneficial genera (Arthrobacter, Pseudomonas, Gemmatimonas, Trichoderma, Mortierella, Penicillium, Chaetomium, etc.), and the reduction of the numbers of detrimental microbes (Alternaria, Phytophthora nicotianae and Fusarium oxysporum). Moreover, CBF amendment improved the stability and complexity of microbial co-occurrence networks. Soil total carbon, microbial structure, and diversity were the most important explanatory factors for the increase of tobacco leaf yield and economic value. Conclusions Collectively, BFs application under rotation regime showed the great potential as a practical and environmentally friendly strategy to alleviate tobacco CCO by providing an optimized soil environment. Graphical abstract
format Article
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issn 1471-2229
language English
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series BMC Plant Biology
spelling doaj-art-37eeb27d702e4efd8752737d9042d9ac2025-08-20T03:04:11ZengBMCBMC Plant Biology1471-22292025-03-0125111610.1186/s12870-025-06266-7Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structureDan Chen0Yujie Zhou1Gang Wang2Kuai Dai3Jiangzhou Li4Xueru Song5Yongxian Xu6Yonghe Cui7Xiaoe Yang8Ministry of Education (MOE) Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang UniversityMinistry of Education (MOE) Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang UniversityMinistry of Education (MOE) Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang UniversityYuxi Tobacco Company, Ltd. of Yunnan ProvinceYuxi Tobacco Company, Ltd. of Yunnan ProvinceYuxi Tobacco Company, Ltd. of Yunnan ProvinceYuxi Tobacco Company, Ltd. of Yunnan ProvinceYuxi Tobacco Company, Ltd. of Yunnan ProvinceMinistry of Education (MOE) Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang UniversityAbstract Background Intensive monoculture poses a serious threat to agricultural sustainable development due to the phenomenon of continuous cropping obstacles. Although organic amendment has been considered an efficient and environmentally friendly solution to mitigate this tough issue, the associated mechanisms remain poorly understood. Here, a two-year field experiment was conducted with the application of four fertilizers, wood, rice straw, compound biochar-based organic fertilizers (WBF, RBF, CBF) and chemical fertilizer (CF) under tobacco rotation with broad bean and oilseed rape, respectively. This work aims to determine how BFs application alleviates tobacco CCO and to further reveal the underlying action mechanisms primarily focusing on the change of soil micro-ecology environments. Results The results depicted that BFs addition decreased tobacco morbidity (by 15.7–85.0%), heavy metals (Cd, V, Cu, Zn) contents in tobacco, and improved tobacco leaf production yield (by 4.5–20.5%), economic value (by 14.6–34.4%) and chemical quality compared with CF. Rhizosphere soil chemical properties and the structure and diversity of microbial communities were enhanced under BFs treatments, reflecting in the growth of bacterial OTUs number, microbial alpha-diversity, the abundances of some beneficial genera (Arthrobacter, Pseudomonas, Gemmatimonas, Trichoderma, Mortierella, Penicillium, Chaetomium, etc.), and the reduction of the numbers of detrimental microbes (Alternaria, Phytophthora nicotianae and Fusarium oxysporum). Moreover, CBF amendment improved the stability and complexity of microbial co-occurrence networks. Soil total carbon, microbial structure, and diversity were the most important explanatory factors for the increase of tobacco leaf yield and economic value. Conclusions Collectively, BFs application under rotation regime showed the great potential as a practical and environmentally friendly strategy to alleviate tobacco CCO by providing an optimized soil environment. Graphical abstracthttps://doi.org/10.1186/s12870-025-06266-7Biochar-based organic fertilizersCrop rotationContinuous cropping obstaclesSoil chemical propertiesRhizosphere microbial community
spellingShingle Dan Chen
Yujie Zhou
Gang Wang
Kuai Dai
Jiangzhou Li
Xueru Song
Yongxian Xu
Yonghe Cui
Xiaoe Yang
Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structure
BMC Plant Biology
Biochar-based organic fertilizers
Crop rotation
Continuous cropping obstacles
Soil chemical properties
Rhizosphere microbial community
title Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structure
title_full Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structure
title_fullStr Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structure
title_full_unstemmed Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structure
title_short Biochar-based organic fertilizer application promotes the alleviation of tobacco (Nicotiana tabacum L.) continuous cropping obstacles by improving soil chemical properties and microbial community structure
title_sort biochar based organic fertilizer application promotes the alleviation of tobacco nicotiana tabacum l continuous cropping obstacles by improving soil chemical properties and microbial community structure
topic Biochar-based organic fertilizers
Crop rotation
Continuous cropping obstacles
Soil chemical properties
Rhizosphere microbial community
url https://doi.org/10.1186/s12870-025-06266-7
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