Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage.
Synthesis of antifreeze proteins (AFPs) is one of the adaptations of psychrophilic yeast to live in cold environments. AFPs demonstrate thermal hysteresis (TH) activity and inhibit the recrystallization of ice (IRI) during periodic temperature fluctuations. In this study, the Antarctic yeast strain...
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Public Library of Science (PLoS)
2025-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0318459 |
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| author | Edyta Majewska Aleksandra Twarda-Clapa Marzena Jędrzejczak-Krzepkowska Anna Kamińska-Dwórznicka Małgorzata Zakłos-Szyda Aneta Monika Białkowska |
| author_facet | Edyta Majewska Aleksandra Twarda-Clapa Marzena Jędrzejczak-Krzepkowska Anna Kamińska-Dwórznicka Małgorzata Zakłos-Szyda Aneta Monika Białkowska |
| author_sort | Edyta Majewska |
| collection | DOAJ |
| description | Synthesis of antifreeze proteins (AFPs) is one of the adaptations of psychrophilic yeast to live in cold environments. AFPs demonstrate thermal hysteresis (TH) activity and inhibit the recrystallization of ice (IRI) during periodic temperature fluctuations. In this study, the Antarctic yeast strain 186, identified as Glaciozyma martinii, was found to synthesize an extracellular, glycosylated ~27 kDa ice-binding protein (GmAFP) exhibiting IRI activity. It is the first evidence of AFP secretion by the psychrophilic yeast Glaciozyma martinii. To scale up protein production, a synthetic gene from a closely related cold-adapted species, Glaciozyma antarctica, was expressed in Pichia pastoris GS115 strain. The recombinant 26.57 kD protein (GaAFP) displayed IRI activity and a cryoprotective effect in food storage. The addition of GaAFP to the stored frozen vegetables and fruits (carrot, kohlrabi, and blueberry) markedly reduced the drip loss during the thawing process and positively affected their structure, with an effect similar to glycerol. Moreover, GaAFP increased the cell survival of Saccharomyces cerevisiae after freezing. The insights from this study provided proof that AFPs from natural sources may serve as competent biodegradable, eco-friendly, non-cytotoxic and biocompatible substitutes for traditional cryoprotectants in enhancing the quality of frozen foods. |
| format | Article |
| id | doaj-art-37e0d75615c141bdae76bd7d14a7c282 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-37e0d75615c141bdae76bd7d14a7c2822025-08-20T01:51:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01203e031845910.1371/journal.pone.0318459Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage.Edyta MajewskaAleksandra Twarda-ClapaMarzena Jędrzejczak-KrzepkowskaAnna Kamińska-DwórznickaMałgorzata Zakłos-SzydaAneta Monika BiałkowskaSynthesis of antifreeze proteins (AFPs) is one of the adaptations of psychrophilic yeast to live in cold environments. AFPs demonstrate thermal hysteresis (TH) activity and inhibit the recrystallization of ice (IRI) during periodic temperature fluctuations. In this study, the Antarctic yeast strain 186, identified as Glaciozyma martinii, was found to synthesize an extracellular, glycosylated ~27 kDa ice-binding protein (GmAFP) exhibiting IRI activity. It is the first evidence of AFP secretion by the psychrophilic yeast Glaciozyma martinii. To scale up protein production, a synthetic gene from a closely related cold-adapted species, Glaciozyma antarctica, was expressed in Pichia pastoris GS115 strain. The recombinant 26.57 kD protein (GaAFP) displayed IRI activity and a cryoprotective effect in food storage. The addition of GaAFP to the stored frozen vegetables and fruits (carrot, kohlrabi, and blueberry) markedly reduced the drip loss during the thawing process and positively affected their structure, with an effect similar to glycerol. Moreover, GaAFP increased the cell survival of Saccharomyces cerevisiae after freezing. The insights from this study provided proof that AFPs from natural sources may serve as competent biodegradable, eco-friendly, non-cytotoxic and biocompatible substitutes for traditional cryoprotectants in enhancing the quality of frozen foods.https://doi.org/10.1371/journal.pone.0318459 |
| spellingShingle | Edyta Majewska Aleksandra Twarda-Clapa Marzena Jędrzejczak-Krzepkowska Anna Kamińska-Dwórznicka Małgorzata Zakłos-Szyda Aneta Monika Białkowska Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage. PLoS ONE |
| title | Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage. |
| title_full | Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage. |
| title_fullStr | Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage. |
| title_full_unstemmed | Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage. |
| title_short | Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage. |
| title_sort | antifreeze proteins produced by antarctic yeast from the genus glaciozyma as cryoprotectants in food storage |
| url | https://doi.org/10.1371/journal.pone.0318459 |
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