Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy
Objective: Diabetic nephropathy is one of the most significant causes of end-stage renal failure and is a common microvascular complication of diabetes (D). Resveratrol (RSV), a natural compound found in grape skins and red wine, has potent antioxidant properties. This study aimed to evaluate the ef...
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Galenos Publishing House
2025-01-01
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| Series: | Gazi Medical Journal |
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| Online Access: | https://gazimedj.com/articles/effects-of-resveratrol-on-the-kidney-in-rats-with-streptozotocin-induced-diabetic-nephropathy/doi/gmj.2024.4232 |
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| author | Duygu Tozcu Çiğdem Özer Aydan Babül Sevim Ercan Ergin Dileköz Gülnur Take Kaplanoğlu Güleser Göktaş |
| author_facet | Duygu Tozcu Çiğdem Özer Aydan Babül Sevim Ercan Ergin Dileköz Gülnur Take Kaplanoğlu Güleser Göktaş |
| author_sort | Duygu Tozcu |
| collection | DOAJ |
| description | Objective: Diabetic nephropathy is one of the most significant causes of end-stage renal failure and is a common microvascular complication of diabetes (D). Resveratrol (RSV), a natural compound found in grape skins and red wine, has potent antioxidant properties. This study aimed to evaluate the effects of RSV in a streptozotocin (STZ)-induced diabetic rat model.
Methods: Animals were divided into four groups: control, RSV, D, and D + RSV. The diabetic group received a single intraperitoneal dose of STZ (65 mg/kg). After 2 weeks, rats with basal blood glucose levels >250 mg/dL were considered diabetic. RSV(10 mg/kg/day) was administered orally by gavage for 8 weeks. Metabolic analyses were conducted throughout the study. At the study’s end, transmission electron microscopy and immunohistochemical analyses were performed. Additionally, the left kidney was isolated and suspended in an organ bath to study the functional changes without damaging the renal artery.
Results: In the study, increased transforming growth factor-beta, fibronectin and inducible nitric oxide synthase immunoreactivity, which are markers of D-induced renal degeneration, were partially reduced by RSV treatment. In group D, decreased endothelial nitric oxide synthase uptake (weak immune reactivity) was observed, whereas this uptake increased with RSV treatment (moderate immune reactivity). Furthermore, both angiotensin II and phenylephrine responses were reduced in group D treated with RSV. Vasodilator responses to acetylcholine were also reduced in this group.
Conclusion: RSV may protect against diabetic nephropathy by modulating key pathways involved in renal degeneration and vascular function and may have potential as a therapeutic agent for slowing disease progression. |
| format | Article |
| id | doaj-art-37dd61913aa54330b56bf7f0c8ea73ff |
| institution | DOAJ |
| issn | 2147-2092 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Gazi Medical Journal |
| spelling | doaj-art-37dd61913aa54330b56bf7f0c8ea73ff2025-08-20T02:41:27ZengGalenos Publishing HouseGazi Medical Journal2147-20922025-01-013619610410.12996/gmj.2024.4232Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic NephropathyDuygu Tozcu0https://orcid.org/0000-0002-3972-5442Çiğdem Özer1https://orcid.org/0000-0002-2705-4522Aydan Babül2https://orcid.org/0000-0001-5554-0010Sevim Ercan3Ergin Dileköz4https://orcid.org/0000-0001-5868-364XGülnur Take Kaplanoğlu5https://orcid.org/0000-0002-3661-3488Güleser Göktaş6https://orcid.org/0000-0001-7989-4346Department of Physiology, Amasya University Faculty of Medicine, Amasya, TürkiyeDepartment of Physiology, Gazi University Faculty of Medicine, Ankara, TürkiyeDepartment of Physiology, Gazi University Faculty of Medicine, Ankara, TürkiyeDepartment of Pharmacology, Gazi University Faculty of Medicine, Ankara, TürkiyeDepartment of Pharmacology, Gazi University Faculty of Medicine, Ankara, TürkiyeDepartment of Histology Embriyology, Gazi University Faculty of Medicine, Ankara, TürkiyeDepartment of Histology Embriyology, Gazi University Faculty of Medicine, Ankara, TürkiyeObjective: Diabetic nephropathy is one of the most significant causes of end-stage renal failure and is a common microvascular complication of diabetes (D). Resveratrol (RSV), a natural compound found in grape skins and red wine, has potent antioxidant properties. This study aimed to evaluate the effects of RSV in a streptozotocin (STZ)-induced diabetic rat model. Methods: Animals were divided into four groups: control, RSV, D, and D + RSV. The diabetic group received a single intraperitoneal dose of STZ (65 mg/kg). After 2 weeks, rats with basal blood glucose levels >250 mg/dL were considered diabetic. RSV(10 mg/kg/day) was administered orally by gavage for 8 weeks. Metabolic analyses were conducted throughout the study. At the study’s end, transmission electron microscopy and immunohistochemical analyses were performed. Additionally, the left kidney was isolated and suspended in an organ bath to study the functional changes without damaging the renal artery. Results: In the study, increased transforming growth factor-beta, fibronectin and inducible nitric oxide synthase immunoreactivity, which are markers of D-induced renal degeneration, were partially reduced by RSV treatment. In group D, decreased endothelial nitric oxide synthase uptake (weak immune reactivity) was observed, whereas this uptake increased with RSV treatment (moderate immune reactivity). Furthermore, both angiotensin II and phenylephrine responses were reduced in group D treated with RSV. Vasodilator responses to acetylcholine were also reduced in this group. Conclusion: RSV may protect against diabetic nephropathy by modulating key pathways involved in renal degeneration and vascular function and may have potential as a therapeutic agent for slowing disease progression.https://gazimedj.com/articles/effects-of-resveratrol-on-the-kidney-in-rats-with-streptozotocin-induced-diabetic-nephropathy/doi/gmj.2024.4232resveratroldiabetic nephropathyproinflammatory cytokinestransmission electron microscoperenal vascular responses |
| spellingShingle | Duygu Tozcu Çiğdem Özer Aydan Babül Sevim Ercan Ergin Dileköz Gülnur Take Kaplanoğlu Güleser Göktaş Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy Gazi Medical Journal resveratrol diabetic nephropathy proinflammatory cytokines transmission electron microscope renal vascular responses |
| title | Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy |
| title_full | Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy |
| title_fullStr | Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy |
| title_full_unstemmed | Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy |
| title_short | Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy |
| title_sort | effects of resveratrol on the kidney in rats with streptozotocin induced diabetic nephropathy |
| topic | resveratrol diabetic nephropathy proinflammatory cytokines transmission electron microscope renal vascular responses |
| url | https://gazimedj.com/articles/effects-of-resveratrol-on-the-kidney-in-rats-with-streptozotocin-induced-diabetic-nephropathy/doi/gmj.2024.4232 |
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