Rosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathways
Abstract There has been a continuous increase in the global incidence of obesity. Rosa roxburghii Tratt (RRT), a plant with medicinal and edible uses, contains active components that can alleviate obesity, indicating an anti-obesity potential. This research sought to investigate the anti-obesity eff...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-12126-z |
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| author | Yihan Ling Wenxi Wang Xingjie Li Xiaomeng Wang Shuwen Zhang Shaolin Huang Heting Zhou Lijun Peng Wenya Yin |
| author_facet | Yihan Ling Wenxi Wang Xingjie Li Xiaomeng Wang Shuwen Zhang Shaolin Huang Heting Zhou Lijun Peng Wenya Yin |
| author_sort | Yihan Ling |
| collection | DOAJ |
| description | Abstract There has been a continuous increase in the global incidence of obesity. Rosa roxburghii Tratt (RRT), a plant with medicinal and edible uses, contains active components that can alleviate obesity, indicating an anti-obesity potential. This research sought to investigate the anti-obesity effects of RRT fruit extract (RRTE) and elucidate the underlying mechanisms and components responsible for its action. RRTE was obtained by enriching the active ingredient in RRT and used to treat HepG2 cells. RRTE alleviated cellular lipid accumulation, reduced reactive oxygen species production, restored cellular mitochondrial function, and enhanced energy metabolism. Network pharmacology combined with molecular docking was used to determine the targets, components, and mechanisms, which were validated through in vitro experiments. p-Coumaric acid and ethyl caffeate were identified as lipid-lowering active components that may reduce lipid accumulation and alleviate obesity through the PI3K-Akt and PPAR signaling pathways. |
| format | Article |
| id | doaj-art-37db2fd9ca294f10a3c639d7aa244be5 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-37db2fd9ca294f10a3c639d7aa244be52025-08-20T03:05:17ZengNature PortfolioScientific Reports2045-23222025-07-0115111710.1038/s41598-025-12126-zRosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathwaysYihan Ling0Wenxi Wang1Xingjie Li2Xiaomeng Wang3Shuwen Zhang4Shaolin Huang5Heting Zhou6Lijun Peng7Wenya Yin8West China School of Public Health and West China Fourth Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversityDepartment of Clinical Nutrition, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of ChinaWest China School of Public Health and West China Fourth Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversityAbstract There has been a continuous increase in the global incidence of obesity. Rosa roxburghii Tratt (RRT), a plant with medicinal and edible uses, contains active components that can alleviate obesity, indicating an anti-obesity potential. This research sought to investigate the anti-obesity effects of RRT fruit extract (RRTE) and elucidate the underlying mechanisms and components responsible for its action. RRTE was obtained by enriching the active ingredient in RRT and used to treat HepG2 cells. RRTE alleviated cellular lipid accumulation, reduced reactive oxygen species production, restored cellular mitochondrial function, and enhanced energy metabolism. Network pharmacology combined with molecular docking was used to determine the targets, components, and mechanisms, which were validated through in vitro experiments. p-Coumaric acid and ethyl caffeate were identified as lipid-lowering active components that may reduce lipid accumulation and alleviate obesity through the PI3K-Akt and PPAR signaling pathways.https://doi.org/10.1038/s41598-025-12126-zRosa roxburghii Tratt fruit extractObesityLipid metabolismMitochondrial functionNetwork PharmacologyPI3K-Akt signaling pathway |
| spellingShingle | Yihan Ling Wenxi Wang Xingjie Li Xiaomeng Wang Shuwen Zhang Shaolin Huang Heting Zhou Lijun Peng Wenya Yin Rosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathways Scientific Reports Rosa roxburghii Tratt fruit extract Obesity Lipid metabolism Mitochondrial function Network Pharmacology PI3K-Akt signaling pathway |
| title | Rosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathways |
| title_full | Rosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathways |
| title_fullStr | Rosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathways |
| title_full_unstemmed | Rosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathways |
| title_short | Rosa roxburghii Tratt fruit extract ameliorate free fatty acid induced steatosis in HepG2 cells through activating PI3K-Akt and PPAR pathways |
| title_sort | rosa roxburghii tratt fruit extract ameliorate free fatty acid induced steatosis in hepg2 cells through activating pi3k akt and ppar pathways |
| topic | Rosa roxburghii Tratt fruit extract Obesity Lipid metabolism Mitochondrial function Network Pharmacology PI3K-Akt signaling pathway |
| url | https://doi.org/10.1038/s41598-025-12126-z |
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