Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles
Abstract Little is known about how cell cycle and autophagy, two fundamental life processes, affect cellular accumulation of nanoparticles. What’s even more tough is that several long-lasting methodological barriers have hindered the progress of related research. Here we firstly show the constructio...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60962-4 |
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| author | Yisha Wang Gan Luo Haiyang Wang Yue Zheng Xiao Xu Wenbin Zhou Junrong Lin Baocheng Chen Yangfu Guo Yifeng Jin Meihua Sui |
| author_facet | Yisha Wang Gan Luo Haiyang Wang Yue Zheng Xiao Xu Wenbin Zhou Junrong Lin Baocheng Chen Yangfu Guo Yifeng Jin Meihua Sui |
| author_sort | Yisha Wang |
| collection | DOAJ |
| description | Abstract Little is known about how cell cycle and autophagy, two fundamental life processes, affect cellular accumulation of nanoparticles. What’s even more tough is that several long-lasting methodological barriers have hindered the progress of related research. Here we firstly show the construction of a multi-functional platform for overcoming existing methodological obstacles through integrating multiple technical approaches including autophagy-related gene 7 knockout to specifically block autophagy, PIP-FUCCI transfection and mitotic shake-off to thoroughly separate cell cycle phases, and 3D reconstruction to stereoscopically evaluate cellular accumulation of nanoparticles. Further application of this platform reveals that after a 2-hour incubation of lipid-based nanoparticles, G2-phase and M-phase cells, two populations previously muddled up together as G2/M-phase cells, respectively exhibited the maximum and minimum nanoparticle accumulation. Meanwhile, our data preliminarily suggest enhanced nanoparticle accumulation by autophagy blockade. Besides cell cycle and autophagy, comprehensive statistical analyses reveal a close association between cellular accumulation of nanoparticles and nanoparticle type. This study not only provides a valuable technical strategy, but uncovers important characteristics of cellular accumulation of nanoparticles, offering new insights for optimization and application of nanomedicines. |
| format | Article |
| id | doaj-art-37ba3f09d9bd4201b77ead8d40e919b8 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-37ba3f09d9bd4201b77ead8d40e919b82025-08-20T03:37:38ZengNature PortfolioNature Communications2041-17232025-07-0116111810.1038/s41467-025-60962-4Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticlesYisha Wang0Gan Luo1Haiyang Wang2Yue Zheng3Xiao Xu4Wenbin Zhou5Junrong Lin6Baocheng Chen7Yangfu Guo8Yifeng Jin9Meihua Sui10School of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Clinical Medicine, Hangzhou Medical CollegeSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineSchool of Basic Medical Sciences and Sir Run Run Shaw Hospital, Zhejiang University School of MedicineAbstract Little is known about how cell cycle and autophagy, two fundamental life processes, affect cellular accumulation of nanoparticles. What’s even more tough is that several long-lasting methodological barriers have hindered the progress of related research. Here we firstly show the construction of a multi-functional platform for overcoming existing methodological obstacles through integrating multiple technical approaches including autophagy-related gene 7 knockout to specifically block autophagy, PIP-FUCCI transfection and mitotic shake-off to thoroughly separate cell cycle phases, and 3D reconstruction to stereoscopically evaluate cellular accumulation of nanoparticles. Further application of this platform reveals that after a 2-hour incubation of lipid-based nanoparticles, G2-phase and M-phase cells, two populations previously muddled up together as G2/M-phase cells, respectively exhibited the maximum and minimum nanoparticle accumulation. Meanwhile, our data preliminarily suggest enhanced nanoparticle accumulation by autophagy blockade. Besides cell cycle and autophagy, comprehensive statistical analyses reveal a close association between cellular accumulation of nanoparticles and nanoparticle type. This study not only provides a valuable technical strategy, but uncovers important characteristics of cellular accumulation of nanoparticles, offering new insights for optimization and application of nanomedicines.https://doi.org/10.1038/s41467-025-60962-4 |
| spellingShingle | Yisha Wang Gan Luo Haiyang Wang Yue Zheng Xiao Xu Wenbin Zhou Junrong Lin Baocheng Chen Yangfu Guo Yifeng Jin Meihua Sui Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles Nature Communications |
| title | Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles |
| title_full | Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles |
| title_fullStr | Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles |
| title_full_unstemmed | Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles |
| title_short | Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles |
| title_sort | evaluating cell cycle and autophagy associated cellular accumulation of lipid based nanoparticles |
| url | https://doi.org/10.1038/s41467-025-60962-4 |
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