Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot
Most orange peels are not utilized and become useless waste that is thrown away. Orange peels contain many nutrients and compounds, such as flavonoids, terpenoids, alkaloids, and essential oils, which are secondary metabolites that act as antibacterial agents. This study intends to investigate the a...
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| Format: | Article |
| Language: | English |
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State Islamic University Sunan Kalijaga
2024-10-01
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| Series: | Biology, Medicine & Natural Product Chemistry |
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| Online Access: | https://sciencebiology.org/index.php/BIOMEDICH/article/view/599 |
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| author | Yumiko Yumiko Suhartomi Suhartomi Sri Wahyuni Nasution Siti Syarifah Ade Pryta R. Simaremare |
| author_facet | Yumiko Yumiko Suhartomi Suhartomi Sri Wahyuni Nasution Siti Syarifah Ade Pryta R. Simaremare |
| author_sort | Yumiko Yumiko |
| collection | DOAJ |
| description | Most orange peels are not utilized and become useless waste that is thrown away. Orange peels contain many nutrients and compounds, such as flavonoids, terpenoids, alkaloids, and essential oils, which are secondary metabolites that act as antibacterial agents. This study intends to investigate the antibacterial activity of sweet orange peel tea against isolated Enterobacteriaceae from a random water depot sample around Universitas Prima Indonesia. This experiment used disc diffusion for antibacterial assay. Meanwhile, the sweet orange peel was brewed using two different methods, including infusion and decoction, in two different masses (3 grams and 5 grams). These sweet orange peel tea formulations were compared to standard (chloramphenicol) and control (distilled water). Enterobacteriaceae was isolated and identified from a random water depot sample around Universitas Prima Indonesia, which included colony identification in EMB agar and MacConkey agar, gram staining, and biochemical test. This study showed that a random water depot sample contaminated by Enterobacteriaceae had properties similar to Citrobacter sp. Sweet orange tea formulation inhibited this isolated Enterobacteriaceae growth (P-Value: 0.010). Antibacterial activity was observed in 3-gram infusion, 5-gram infusion, and 5-gram decoction. However, the antibacterial activity was not better than the standard (chloramphenicol). Overall, it can be concluded that the sweet orange peel tea as infusion or decoction has weak antibacterial activity against Enterobacteriaceae bacteria isolated from water depots, which had some properties similar to Citrobacter sp. |
| format | Article |
| id | doaj-art-37a989b401d847fa9eebf23ab6306e74 |
| institution | DOAJ |
| issn | 2089-6514 2540-9328 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | State Islamic University Sunan Kalijaga |
| record_format | Article |
| series | Biology, Medicine & Natural Product Chemistry |
| spelling | doaj-art-37a989b401d847fa9eebf23ab6306e742025-08-20T02:46:05ZengState Islamic University Sunan KalijagaBiology, Medicine & Natural Product Chemistry2089-65142540-93282024-10-0113244945810.14421/biomedich.2024.132.449-458294Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water DepotYumiko Yumiko0Suhartomi Suhartomi1Sri Wahyuni Nasution2Siti Syarifah3Ade Pryta R. Simaremare4Universitas Prima IndonesiaUniversitas Prima IndonesiaUniversitas Prima IndonesiaUniversitas Sumatera UtaraUniversitas HKBP NommensenMost orange peels are not utilized and become useless waste that is thrown away. Orange peels contain many nutrients and compounds, such as flavonoids, terpenoids, alkaloids, and essential oils, which are secondary metabolites that act as antibacterial agents. This study intends to investigate the antibacterial activity of sweet orange peel tea against isolated Enterobacteriaceae from a random water depot sample around Universitas Prima Indonesia. This experiment used disc diffusion for antibacterial assay. Meanwhile, the sweet orange peel was brewed using two different methods, including infusion and decoction, in two different masses (3 grams and 5 grams). These sweet orange peel tea formulations were compared to standard (chloramphenicol) and control (distilled water). Enterobacteriaceae was isolated and identified from a random water depot sample around Universitas Prima Indonesia, which included colony identification in EMB agar and MacConkey agar, gram staining, and biochemical test. This study showed that a random water depot sample contaminated by Enterobacteriaceae had properties similar to Citrobacter sp. Sweet orange tea formulation inhibited this isolated Enterobacteriaceae growth (P-Value: 0.010). Antibacterial activity was observed in 3-gram infusion, 5-gram infusion, and 5-gram decoction. However, the antibacterial activity was not better than the standard (chloramphenicol). Overall, it can be concluded that the sweet orange peel tea as infusion or decoction has weak antibacterial activity against Enterobacteriaceae bacteria isolated from water depots, which had some properties similar to Citrobacter sp.https://sciencebiology.org/index.php/BIOMEDICH/article/view/599sweet orangedecoctionenterobacteriaceaecitrobacterinfusion |
| spellingShingle | Yumiko Yumiko Suhartomi Suhartomi Sri Wahyuni Nasution Siti Syarifah Ade Pryta R. Simaremare Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot Biology, Medicine & Natural Product Chemistry sweet orange decoction enterobacteriaceae citrobacter infusion |
| title | Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot |
| title_full | Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot |
| title_fullStr | Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot |
| title_full_unstemmed | Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot |
| title_short | Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot |
| title_sort | antibacterial activity of sweet orange citrus sinensis peel tea against enterobacteriaceae isolated from a water depot |
| topic | sweet orange decoction enterobacteriaceae citrobacter infusion |
| url | https://sciencebiology.org/index.php/BIOMEDICH/article/view/599 |
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