The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women
The study aims to evaluate the significant role of interleukin 15 (IL-15), IL-22, IL-37, and Caspase 9 gene expression in polycystic ovary syndrome (PCOS), focusing on the underlying mechanisms and potential diagnostic or therapeutic implications. Peripheral blood has been collected, and serum was s...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X2500006X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573204148781056 |
|---|---|
| author | Noor A. Mohammed Ghassan M. Sulaiman Hazima M. Alabassi Khalil A.A. Khalil Elsadig M. Ahmed |
| author_facet | Noor A. Mohammed Ghassan M. Sulaiman Hazima M. Alabassi Khalil A.A. Khalil Elsadig M. Ahmed |
| author_sort | Noor A. Mohammed |
| collection | DOAJ |
| description | The study aims to evaluate the significant role of interleukin 15 (IL-15), IL-22, IL-37, and Caspase 9 gene expression in polycystic ovary syndrome (PCOS), focusing on the underlying mechanisms and potential diagnostic or therapeutic implications. Peripheral blood has been collected, and serum was separated for the evaluation of the serum IL-15, IL-22, and IL-37. The ELISA technique has been carried out to determine the serum levels of understudied factors mentioned above in Iraqi women patients diagnosed with PCOS (No. = 90) via a specialized gynecologist and healthy fertile women (No. = 48) as a control group. In addition, a genetic study on the expression of the caspase 9 gene in these patients had been performed. The data reveals statistically significant differences in interleukin levels in PCOS patients versus the control group. Specifically, the PCOS group exhibits significantly higher levels of IL-15 and IL-22 as compared to the control group. Conversely, the PCOS group shows significantly lower levels of IL-37 compared to the control group. The results showed no statistically significant difference in the mean expression of the Caspase 9 gene when comparing these fold graduations. However, it’s worth noting that a higher fold frequency was observed in both the PCOS and control groups, with 57.1 % and 60 %, respectively, having folds less than 1. The distribution of folds varied across other categories was also addressed. Additionally, there was a notable difference in the frequency of 11.4 % in the PCOS group compared to 2 % in the control group for folds greater than 9. The findings suggest that interleukins, particularly IL-22 and IL-37, hold promise as diagnostic markers for distinguishing PCOS from healthy conditions. However, the potential diagnostic utility of the Caspase 9 gene expression was not confirmed in this study. |
| format | Article |
| id | doaj-art-37a9834984b1454184910afeef667bf5 |
| institution | Kabale University |
| issn | 1687-157X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj-art-37a9834984b1454184910afeef667bf52025-02-02T05:26:59ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2025-03-01231100462The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi womenNoor A. Mohammed0Ghassan M. Sulaiman1Hazima M. Alabassi2Khalil A.A. Khalil3Elsadig M. Ahmed4Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, Iraq; Corresponding author.Department of Biology, College of Education for Pure Science, Ibn. Al-Haitham, University of Baghdad, Baghdad, IraqDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, 61922 P.O. Box 551, Saudi Arabia; Department of Medical Laboratory Sciences, Faculty of Medicine and Health Sciences, Hodeidah University, Hodeidah, YemenDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, 61922 P.O. Box 551, Saudi Arabia; Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Elmam El Mahdi, Kosti, 209 P.O. Box 27711, SudanThe study aims to evaluate the significant role of interleukin 15 (IL-15), IL-22, IL-37, and Caspase 9 gene expression in polycystic ovary syndrome (PCOS), focusing on the underlying mechanisms and potential diagnostic or therapeutic implications. Peripheral blood has been collected, and serum was separated for the evaluation of the serum IL-15, IL-22, and IL-37. The ELISA technique has been carried out to determine the serum levels of understudied factors mentioned above in Iraqi women patients diagnosed with PCOS (No. = 90) via a specialized gynecologist and healthy fertile women (No. = 48) as a control group. In addition, a genetic study on the expression of the caspase 9 gene in these patients had been performed. The data reveals statistically significant differences in interleukin levels in PCOS patients versus the control group. Specifically, the PCOS group exhibits significantly higher levels of IL-15 and IL-22 as compared to the control group. Conversely, the PCOS group shows significantly lower levels of IL-37 compared to the control group. The results showed no statistically significant difference in the mean expression of the Caspase 9 gene when comparing these fold graduations. However, it’s worth noting that a higher fold frequency was observed in both the PCOS and control groups, with 57.1 % and 60 %, respectively, having folds less than 1. The distribution of folds varied across other categories was also addressed. Additionally, there was a notable difference in the frequency of 11.4 % in the PCOS group compared to 2 % in the control group for folds greater than 9. The findings suggest that interleukins, particularly IL-22 and IL-37, hold promise as diagnostic markers for distinguishing PCOS from healthy conditions. However, the potential diagnostic utility of the Caspase 9 gene expression was not confirmed in this study.http://www.sciencedirect.com/science/article/pii/S1687157X2500006XInterleukinsIL-15IL-22IL-37Caspase 9 genePolycystic Ovary Syndrome |
| spellingShingle | Noor A. Mohammed Ghassan M. Sulaiman Hazima M. Alabassi Khalil A.A. Khalil Elsadig M. Ahmed The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women Journal of Genetic Engineering and Biotechnology Interleukins IL-15 IL-22 IL-37 Caspase 9 gene Polycystic Ovary Syndrome |
| title | The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women |
| title_full | The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women |
| title_fullStr | The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women |
| title_full_unstemmed | The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women |
| title_short | The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women |
| title_sort | significant role of il 15 il 22 il 37 and caspase 9 in polycystic ovary syndrome a case control study in a sample of iraqi women |
| topic | Interleukins IL-15 IL-22 IL-37 Caspase 9 gene Polycystic Ovary Syndrome |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X2500006X |
| work_keys_str_mv | AT nooramohammed thesignificantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT ghassanmsulaiman thesignificantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT hazimamalabassi thesignificantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT khalilaakhalil thesignificantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT elsadigmahmed thesignificantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT nooramohammed significantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT ghassanmsulaiman significantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT hazimamalabassi significantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT khalilaakhalil significantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen AT elsadigmahmed significantroleofil15il22il37andcaspase9inpolycysticovarysyndromeacasecontrolstudyinasampleofiraqiwomen |