Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical Preparations
Four simple, sensitive, accurate and rapid visible spectrophotometric methods (A, B, C and D) have been developed for the estimation of sulfadoxine in pharmaceutical preparations. They are based on the diazotization of sulfadoxine with sodium nitrite and hydrochloric acid followed by coupling with N...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/289723 |
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| author | Sangita Sharma Madhurjya Neog Vipul Prajapati Hiren Patel Dipti Dabhi |
| author_facet | Sangita Sharma Madhurjya Neog Vipul Prajapati Hiren Patel Dipti Dabhi |
| author_sort | Sangita Sharma |
| collection | DOAJ |
| description | Four simple, sensitive, accurate and rapid visible spectrophotometric methods (A, B, C and D) have been developed for the estimation of sulfadoxine in pharmaceutical preparations. They are based on the diazotization of sulfadoxine with sodium nitrite and hydrochloric acid followed by coupling with N-(1-naphthyl) ethylenediamine dihydrochloride (Method A) to form pink coloured chromogen, diphenylamine (Method B) to form light pink coloured chromogen, chromotropic acid (in alkaline medium) (Method C) to form orange coloured chromogen, Resorcinol (in alkaline medium) (Method D) to form light orange coloured chromogen and exhibiting absorption maxima (λmax) at 536 nm, 524 nm, 520 nm and 496 nm respectively. The coloured chromogens formed are stable for more than 2 h. Beer’s law was obeyed in the concentration range of 1.0 - 5.0 μg/mL in Method A , 5.0 - 25.0 μg/mL in Method B, 5.0 - 25.0 μg/mL in Method C and 4.0 - 8.0 μg/mL in Method D respectively. The results of the three analysis have been validated statistically and by recovery studies. The results obtained in the proposed methods are in good agreements with labeled amounts, when marketed pharmaceutical preparations are analyzed. |
| format | Article |
| id | doaj-art-379e3a71e56f42c49666ba74f1edaf0e |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-379e3a71e56f42c49666ba74f1edaf0e2025-02-03T01:12:11ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-01741246125310.1155/2010/289723Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical PreparationsSangita Sharma0Madhurjya Neog1Vipul Prajapati2Hiren Patel3Dipti Dabhi4Department of Chemistry, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, IndiaDepartment of Chemistry, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, IndiaDepartment of Chemistry, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, IndiaDepartment of Chemistry, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, IndiaDepartment of Chemistry, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, IndiaFour simple, sensitive, accurate and rapid visible spectrophotometric methods (A, B, C and D) have been developed for the estimation of sulfadoxine in pharmaceutical preparations. They are based on the diazotization of sulfadoxine with sodium nitrite and hydrochloric acid followed by coupling with N-(1-naphthyl) ethylenediamine dihydrochloride (Method A) to form pink coloured chromogen, diphenylamine (Method B) to form light pink coloured chromogen, chromotropic acid (in alkaline medium) (Method C) to form orange coloured chromogen, Resorcinol (in alkaline medium) (Method D) to form light orange coloured chromogen and exhibiting absorption maxima (λmax) at 536 nm, 524 nm, 520 nm and 496 nm respectively. The coloured chromogens formed are stable for more than 2 h. Beer’s law was obeyed in the concentration range of 1.0 - 5.0 μg/mL in Method A , 5.0 - 25.0 μg/mL in Method B, 5.0 - 25.0 μg/mL in Method C and 4.0 - 8.0 μg/mL in Method D respectively. The results of the three analysis have been validated statistically and by recovery studies. The results obtained in the proposed methods are in good agreements with labeled amounts, when marketed pharmaceutical preparations are analyzed.http://dx.doi.org/10.1155/2010/289723 |
| spellingShingle | Sangita Sharma Madhurjya Neog Vipul Prajapati Hiren Patel Dipti Dabhi Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical Preparations E-Journal of Chemistry |
| title | Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical Preparations |
| title_full | Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical Preparations |
| title_fullStr | Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical Preparations |
| title_full_unstemmed | Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical Preparations |
| title_short | Spectrophotometric Estimation of Sulfadoxine in Pharmaceutical Preparations |
| title_sort | spectrophotometric estimation of sulfadoxine in pharmaceutical preparations |
| url | http://dx.doi.org/10.1155/2010/289723 |
| work_keys_str_mv | AT sangitasharma spectrophotometricestimationofsulfadoxineinpharmaceuticalpreparations AT madhurjyaneog spectrophotometricestimationofsulfadoxineinpharmaceuticalpreparations AT vipulprajapati spectrophotometricestimationofsulfadoxineinpharmaceuticalpreparations AT hirenpatel spectrophotometricestimationofsulfadoxineinpharmaceuticalpreparations AT diptidabhi spectrophotometricestimationofsulfadoxineinpharmaceuticalpreparations |