Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media
Haemophilus influenzae is a Gram-negative opportunistic bacterial pathogen of the human respiratory tract. This study describes the prevalence, serotype distribution, and susceptibility profiles of H. influenzae strains isolated from the nasopharynx of school children with acute otitis media (AOM) i...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/5391291 |
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| author | Dodi Safari Daniel Joko Wahyono Wisnu Tafroji Anton Budhi Darmawan Yayah Winarti Wahyu Dwi Kusdaryanto Wisiva Tofriska Paramaiswari Hendro Pramono Meyta Pratiwi Muhamad Riza Chamadi |
| author_facet | Dodi Safari Daniel Joko Wahyono Wisnu Tafroji Anton Budhi Darmawan Yayah Winarti Wahyu Dwi Kusdaryanto Wisiva Tofriska Paramaiswari Hendro Pramono Meyta Pratiwi Muhamad Riza Chamadi |
| author_sort | Dodi Safari |
| collection | DOAJ |
| description | Haemophilus influenzae is a Gram-negative opportunistic bacterial pathogen of the human respiratory tract. This study describes the prevalence, serotype distribution, and susceptibility profiles of H. influenzae strains isolated from the nasopharynx of school children with acute otitis media (AOM) in Banyumas Regency, Central Java, Indonesia. H. influenzae was isolated from nasopharyngeal swab specimens using chocolate agar plates supplemented with IsoVitaleX and bacitracin. Serotyping was performed using quantitative polymerase chain reaction. Antimicrobial susceptibility profiles were determined using a microdilution broth assay. H. influenzae was present in 69.7% of samples (85/122). Nontypeable H. influenzae (NHTi) was the most common serotype (95.3%), followed by H. influenzae type b (3.5%) and H. influenzae type f (1.2%). All the H. influenzae isolates were susceptible to levofloxacin, ceftriaxone, imipenem, meropenem, cefuroxime, and cefixime. Most isolates were susceptible to sparfloxacin (99%), cefepime (99%), amoxicillin/clavulanic acid 2 : 1 (99%), ampicillin/sulbactam 2 : 1 (96%), chloramphenicol (94%), tetracycline (93%), ampicillin (87%), and clarithromycin (82%). Nineteen percent of the isolates were resistant to cotrimoxazole, and 11% of the isolates were resistant to ampicillin. This study showed that H. influenzae carriage among samples was dominated by NTHi and less susceptible to cotrimoxazole. |
| format | Article |
| id | doaj-art-379bc0f9f0ed43dd889ecb9fdb8ee676 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-379bc0f9f0ed43dd889ecb9fdb8ee6762025-02-03T05:53:35ZengWileyInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/5391291Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis MediaDodi Safari0Daniel Joko Wahyono1Wisnu Tafroji2Anton Budhi Darmawan3Yayah Winarti4Wahyu Dwi Kusdaryanto5Wisiva Tofriska Paramaiswari6Hendro Pramono7Meyta Pratiwi8Muhamad Riza Chamadi9Eijkman Institute for Molecular Biology/Pusat Riset Biologi Molekuler EijkmanJenderal Soedirman UniversityEijkman Institute for Molecular Biology/Pusat Riset Biologi Molekuler EijkmanDepartment of OtorhinolaryngologyEijkman Institute for Molecular Biology/Pusat Riset Biologi Molekuler EijkmanDepartment of OtorhinolaryngologyEijkman Institute for Molecular Biology/Pusat Riset Biologi Molekuler EijkmanJenderal Soedirman UniversityJenderal Soedirman UniversityJenderal Soedirman UniversityHaemophilus influenzae is a Gram-negative opportunistic bacterial pathogen of the human respiratory tract. This study describes the prevalence, serotype distribution, and susceptibility profiles of H. influenzae strains isolated from the nasopharynx of school children with acute otitis media (AOM) in Banyumas Regency, Central Java, Indonesia. H. influenzae was isolated from nasopharyngeal swab specimens using chocolate agar plates supplemented with IsoVitaleX and bacitracin. Serotyping was performed using quantitative polymerase chain reaction. Antimicrobial susceptibility profiles were determined using a microdilution broth assay. H. influenzae was present in 69.7% of samples (85/122). Nontypeable H. influenzae (NHTi) was the most common serotype (95.3%), followed by H. influenzae type b (3.5%) and H. influenzae type f (1.2%). All the H. influenzae isolates were susceptible to levofloxacin, ceftriaxone, imipenem, meropenem, cefuroxime, and cefixime. Most isolates were susceptible to sparfloxacin (99%), cefepime (99%), amoxicillin/clavulanic acid 2 : 1 (99%), ampicillin/sulbactam 2 : 1 (96%), chloramphenicol (94%), tetracycline (93%), ampicillin (87%), and clarithromycin (82%). Nineteen percent of the isolates were resistant to cotrimoxazole, and 11% of the isolates were resistant to ampicillin. This study showed that H. influenzae carriage among samples was dominated by NTHi and less susceptible to cotrimoxazole.http://dx.doi.org/10.1155/2022/5391291 |
| spellingShingle | Dodi Safari Daniel Joko Wahyono Wisnu Tafroji Anton Budhi Darmawan Yayah Winarti Wahyu Dwi Kusdaryanto Wisiva Tofriska Paramaiswari Hendro Pramono Meyta Pratiwi Muhamad Riza Chamadi Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media International Journal of Microbiology |
| title | Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media |
| title_full | Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media |
| title_fullStr | Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media |
| title_full_unstemmed | Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media |
| title_short | Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media |
| title_sort | serotype distribution and antimicrobial resistance profile of haemophilus influenzae isolated from school children with acute otitis media |
| url | http://dx.doi.org/10.1155/2022/5391291 |
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