Characterization and pathogenicity of a novel avian orthoreovirus in China
IntroductionAvian orthoreovirus (ARV) is a significant pathogen causing viral arthritis, leading to substantial economic losses in the poultry industry worldwide.MethodsA novel ARV strain, designated FJ202311, was isolated from a broiler farm in Fujian Province, China. Whole-genome sequencing was co...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1529351/full |
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| author | Shunyan Chen Jialin Yang Li Li Yawei Guo Shenghua Yang Zetao Su Sucan Zhao Xuesong Li Wencheng Lin Yunping Du Lijuan Yin Lianxiang Wang Feng Chen |
| author_facet | Shunyan Chen Jialin Yang Li Li Yawei Guo Shenghua Yang Zetao Su Sucan Zhao Xuesong Li Wencheng Lin Yunping Du Lijuan Yin Lianxiang Wang Feng Chen |
| author_sort | Shunyan Chen |
| collection | DOAJ |
| description | IntroductionAvian orthoreovirus (ARV) is a significant pathogen causing viral arthritis, leading to substantial economic losses in the poultry industry worldwide.MethodsA novel ARV strain, designated FJ202311, was isolated from a broiler farm in Fujian Province, China. Whole-genome sequencing was conducted using next-generation sequencing with MGI technology, and phylogenetic analysis of the sigma C amino acid sequence was performed. Comparative sequence analysis identified unique genetic features of FJ202311. Pathogenicity studies were carried out by inoculating broilers with the isolated strain and monitoring clinical signs, weight gain, and histopathological changes.ResultsThe complete genome of FJ202311 was determined to be 23,495 base pairs in length, encoding 12 major proteins. Phylogenetic analysis revealed that FJ202311 forms a distinct genotypic cluster, exhibiting only 47.1% to 59.3% sequence identity to 16 reference ARV strains. Notably, 50 unique amino acid substitutions were identified in the sigma C protein. Pathogenicity tests demonstrated that FJ202311 caused severe arthritis and tenosynovitis in broilers. Infected birds exhibited significant weight loss compared to controls, with reductions of 11.78% and 8.93% at 14 and 21 days post-infection, respectively.DiscussionThis study highlights the unique molecular and pathogenic characteristics of the novel ARV strain FJ202311, contributing to our understanding of ARV diversity and epidemiology in China. These findings underscore the importance of continuous monitoring and provide insights for developing improved prevention and control strategies against ARV. |
| format | Article |
| id | doaj-art-379ad825dc2e4b46894b9b1954e7e839 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-379ad825dc2e4b46894b9b1954e7e8392025-01-09T06:10:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.15293511529351Characterization and pathogenicity of a novel avian orthoreovirus in ChinaShunyan Chen0Jialin Yang1Li Li2Yawei Guo3Shenghua Yang4Zetao Su5Sucan Zhao6Xuesong Li7Wencheng Lin8Yunping Du9Lijuan Yin10Lianxiang Wang11Feng Chen12College of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaIntroductionAvian orthoreovirus (ARV) is a significant pathogen causing viral arthritis, leading to substantial economic losses in the poultry industry worldwide.MethodsA novel ARV strain, designated FJ202311, was isolated from a broiler farm in Fujian Province, China. Whole-genome sequencing was conducted using next-generation sequencing with MGI technology, and phylogenetic analysis of the sigma C amino acid sequence was performed. Comparative sequence analysis identified unique genetic features of FJ202311. Pathogenicity studies were carried out by inoculating broilers with the isolated strain and monitoring clinical signs, weight gain, and histopathological changes.ResultsThe complete genome of FJ202311 was determined to be 23,495 base pairs in length, encoding 12 major proteins. Phylogenetic analysis revealed that FJ202311 forms a distinct genotypic cluster, exhibiting only 47.1% to 59.3% sequence identity to 16 reference ARV strains. Notably, 50 unique amino acid substitutions were identified in the sigma C protein. Pathogenicity tests demonstrated that FJ202311 caused severe arthritis and tenosynovitis in broilers. Infected birds exhibited significant weight loss compared to controls, with reductions of 11.78% and 8.93% at 14 and 21 days post-infection, respectively.DiscussionThis study highlights the unique molecular and pathogenic characteristics of the novel ARV strain FJ202311, contributing to our understanding of ARV diversity and epidemiology in China. These findings underscore the importance of continuous monitoring and provide insights for developing improved prevention and control strategies against ARV.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1529351/fullavian orthoreovirusisolationgenomic analysisphylogenetic analysispathogenicity |
| spellingShingle | Shunyan Chen Jialin Yang Li Li Yawei Guo Shenghua Yang Zetao Su Sucan Zhao Xuesong Li Wencheng Lin Yunping Du Lijuan Yin Lianxiang Wang Feng Chen Characterization and pathogenicity of a novel avian orthoreovirus in China Frontiers in Microbiology avian orthoreovirus isolation genomic analysis phylogenetic analysis pathogenicity |
| title | Characterization and pathogenicity of a novel avian orthoreovirus in China |
| title_full | Characterization and pathogenicity of a novel avian orthoreovirus in China |
| title_fullStr | Characterization and pathogenicity of a novel avian orthoreovirus in China |
| title_full_unstemmed | Characterization and pathogenicity of a novel avian orthoreovirus in China |
| title_short | Characterization and pathogenicity of a novel avian orthoreovirus in China |
| title_sort | characterization and pathogenicity of a novel avian orthoreovirus in china |
| topic | avian orthoreovirus isolation genomic analysis phylogenetic analysis pathogenicity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1529351/full |
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