Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i>
Utilizing <i>Rhodopseudomonas palustris</i> (<i>R. pal</i>), this study constructed a dual-chamber microbial electrosynthesis system, based on microbial electrolysis cells, that was capable of producing lycopene. Cultivation within the electrosynthesis chamber yielded a lycop...
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2024-11-01
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| author | Ningxin Huang Zhengxiao Wang Xiao Xiao Te’er Gai Dongyue Zhao Lu Liu Wei Wu |
| author_facet | Ningxin Huang Zhengxiao Wang Xiao Xiao Te’er Gai Dongyue Zhao Lu Liu Wei Wu |
| author_sort | Ningxin Huang |
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| description | Utilizing <i>Rhodopseudomonas palustris</i> (<i>R. pal</i>), this study constructed a dual-chamber microbial electrosynthesis system, based on microbial electrolysis cells, that was capable of producing lycopene. Cultivation within the electrosynthesis chamber yielded a lycopene concentration of 282.3722 mg/L when the optical density (OD) reached 0.6, which was four times greater than that produced by original strains. The mutant strain showed significantly higher levels of extracted riboflavin compared to the wild-type strain, and the riboflavin content of the mutant strain was 61.081 mg/L, which was more than 10 times that of the original strain. Furthermore, sequencing and analyses were performed on the mutant strains observed during the experiment. The results indicated differences in antibiotic resistance genes, carbohydrate metabolism-related genes, and the frequencies of functional genes between the mutant and original strains. The mutant strain displayed potential advantages in specific antibiotic resistance and carbohydrate degradation capabilities, likely attributable to its adaptation to electrogenic growth conditions. Moreover, the mutant strain demonstrated an enrichment of gene frequencies associated with transcriptional regulation, signal transduction, and amino acid metabolism, suggesting a complex genetic adaptation to electrogenic environments. This study presents a novel approach for the efficient and energy-conserving production of lycopene while also providing deeper insights into the genetic basis of electro-resistance genes. |
| format | Article |
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| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-378afea2e6944839a53e2082ec1749d42025-08-20T01:55:41ZengMDPI AGFoods2304-81582024-11-011323381110.3390/foods13233811Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i>Ningxin Huang0Zhengxiao Wang1Xiao Xiao2Te’er Gai3Dongyue Zhao4Lu Liu5Wei Wu6College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaAdvanced Agri-Tech Institute, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaUtilizing <i>Rhodopseudomonas palustris</i> (<i>R. pal</i>), this study constructed a dual-chamber microbial electrosynthesis system, based on microbial electrolysis cells, that was capable of producing lycopene. Cultivation within the electrosynthesis chamber yielded a lycopene concentration of 282.3722 mg/L when the optical density (OD) reached 0.6, which was four times greater than that produced by original strains. The mutant strain showed significantly higher levels of extracted riboflavin compared to the wild-type strain, and the riboflavin content of the mutant strain was 61.081 mg/L, which was more than 10 times that of the original strain. Furthermore, sequencing and analyses were performed on the mutant strains observed during the experiment. The results indicated differences in antibiotic resistance genes, carbohydrate metabolism-related genes, and the frequencies of functional genes between the mutant and original strains. The mutant strain displayed potential advantages in specific antibiotic resistance and carbohydrate degradation capabilities, likely attributable to its adaptation to electrogenic growth conditions. Moreover, the mutant strain demonstrated an enrichment of gene frequencies associated with transcriptional regulation, signal transduction, and amino acid metabolism, suggesting a complex genetic adaptation to electrogenic environments. This study presents a novel approach for the efficient and energy-conserving production of lycopene while also providing deeper insights into the genetic basis of electro-resistance genes.https://www.mdpi.com/2304-8158/13/23/3811microbial electrosynthesis<i>Rhodopseudomonas palustris</i>lycopene productionmutant strainsgene analysis |
| spellingShingle | Ningxin Huang Zhengxiao Wang Xiao Xiao Te’er Gai Dongyue Zhao Lu Liu Wei Wu Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i> Foods microbial electrosynthesis <i>Rhodopseudomonas palustris</i> lycopene production mutant strains gene analysis |
| title | Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i> |
| title_full | Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i> |
| title_fullStr | Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i> |
| title_full_unstemmed | Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i> |
| title_short | Utilizing Microbial Electrochemical Methods to Enhance Lycopene Production in <i>Rhodopseudomonas palustris</i> |
| title_sort | utilizing microbial electrochemical methods to enhance lycopene production in i rhodopseudomonas palustris i |
| topic | microbial electrosynthesis <i>Rhodopseudomonas palustris</i> lycopene production mutant strains gene analysis |
| url | https://www.mdpi.com/2304-8158/13/23/3811 |
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