Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycin
Based on a real-time PCR technology using an original-design system of allele-specific, fluorophore-labelled primers complementary to the sequence of a fluorescence quencher, the authors developed a method and test system prototypes for the rapid determination of Mycobacterium tuberculosis (MBT) DNA...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2014-12-01
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| Series: | Туберкулез и болезни лёгких |
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| Online Access: | https://www.tibl-journal.com/jour/article/view/320 |
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| author | Yu. S. Alyapkina Ya. I. Alekseev D. A. Varlamov L. V. Domotenko L. K. Shipina M. A. Vladimirsky |
| author_facet | Yu. S. Alyapkina Ya. I. Alekseev D. A. Varlamov L. V. Domotenko L. K. Shipina M. A. Vladimirsky |
| author_sort | Yu. S. Alyapkina |
| collection | DOAJ |
| description | Based on a real-time PCR technology using an original-design system of allele-specific, fluorophore-labelled primers complementary to the sequence of a fluorescence quencher, the authors developed a method and test system prototypes for the rapid determination of Mycobacterium tuberculosis (MBT) DNA mutations responsible for resistance to reserve anti-TB drugs, such as fluoroquinolones, amikacin, and capreomycin.To study drug susceptibility, an Amplitube-MDR-RV molecular genetic test system was used to investigate sputum smears from 68 patients with destructive pulmonary tuberculosis who had been long treated with first- and second-line drugs (53 patients). These patients excreted multidrug-resistant MBT in 51 (75%) cases. All obtained MBT DNA samples were also examined for resistance to fluoroquinolones, amikacin, and capreomycin, by employing both the developed method and prototype reagent kits and by conventional culture methods. Molecular genetic analysis established that the study had a sensitivity of 90% and a specificity of 96%. |
| format | Article |
| id | doaj-art-37865e5225a8436a96bb9e51e1b60b05 |
| institution | OA Journals |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| publishDate | 2014-12-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj-art-37865e5225a8436a96bb9e51e1b60b052025-08-20T02:05:57ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062014-12-010126975320Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycinYu. S. Alyapkina0Ya. I. Alekseev1D. A. Varlamov2L. V. Domotenko3L. K. Shipina4M. A. Vladimirsky5НПФ «Синтол»; НИИ фтизиопульмонологии ГБОУ ВПО «Первый МГМУ им. И. М. Сеченова»НПФ «Синтол»НПФ «Синтол»НИИ прикладной микробиологии Роспотребнадзора РФ, г. МоскваНИИ фтизиопульмонологии ГБОУ ВПО «Первый МГМУ им. И. М. Сеченова»НИИ фтизиопульмонологии ГБОУ ВПО «Первый МГМУ им. И. М. Сеченова»Based on a real-time PCR technology using an original-design system of allele-specific, fluorophore-labelled primers complementary to the sequence of a fluorescence quencher, the authors developed a method and test system prototypes for the rapid determination of Mycobacterium tuberculosis (MBT) DNA mutations responsible for resistance to reserve anti-TB drugs, such as fluoroquinolones, amikacin, and capreomycin.To study drug susceptibility, an Amplitube-MDR-RV molecular genetic test system was used to investigate sputum smears from 68 patients with destructive pulmonary tuberculosis who had been long treated with first- and second-line drugs (53 patients). These patients excreted multidrug-resistant MBT in 51 (75%) cases. All obtained MBT DNA samples were also examined for resistance to fluoroquinolones, amikacin, and capreomycin, by employing both the developed method and prototype reagent kits and by conventional culture methods. Molecular genetic analysis established that the study had a sensitivity of 90% and a specificity of 96%.https://www.tibl-journal.com/jour/article/view/320drug resistancetuberculosisreserve antituberculosis drugs |
| spellingShingle | Yu. S. Alyapkina Ya. I. Alekseev D. A. Varlamov L. V. Domotenko L. K. Shipina M. A. Vladimirsky Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycin Туберкулез и болезни лёгких drug resistance tuberculosis reserve antituberculosis drugs |
| title | Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycin |
| title_full | Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycin |
| title_fullStr | Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycin |
| title_full_unstemmed | Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycin |
| title_short | Development of real-time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs: fluoroquinolones, amikacin, and capreomycin |
| title_sort | development of real time pcr technology for the rapid detection of mycobacterium tuberculosis resistance to reserve antituberculosis drugs fluoroquinolones amikacin and capreomycin |
| topic | drug resistance tuberculosis reserve antituberculosis drugs |
| url | https://www.tibl-journal.com/jour/article/view/320 |
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