Titration-WB: A methodology for accurate quantitative protein determination overcoming reproducibility errors.

Western blotting (WB) is a cornerstone technique for protein detection and quantification in molecular biology. However, its semi-quantitative nature, reliance on housekeeping protein normalization, and susceptibility to technical variability often undermine data accuracy and reproducibility. To add...

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Bibliographic Details
Main Authors: Alice Maestri, Ewa Ehrenborg, Olivera Werngren, Maria Olin, Carolina E Hagberg, Matteo Pedrelli, Paolo Parini
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0325052
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