Titration-WB: A methodology for accurate quantitative protein determination overcoming reproducibility errors.
Western blotting (WB) is a cornerstone technique for protein detection and quantification in molecular biology. However, its semi-quantitative nature, reliance on housekeeping protein normalization, and susceptibility to technical variability often undermine data accuracy and reproducibility. To add...
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| Main Authors: | Alice Maestri, Ewa Ehrenborg, Olivera Werngren, Maria Olin, Carolina E Hagberg, Matteo Pedrelli, Paolo Parini |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0325052 |
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