Protocol for induction, maintenance, and exit from embryo dormancy in mice
Summary: Embryonic dormancy (diapause) is a reproductive adaptation that allows some mammalian species to prolong pregnancy and delay birth by temporarily suspending embryonic development just before implantation. Here, we present a step-by-step protocol for inducing and maintaining embryonic diapau...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002199 |
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| author | Rui Chen Rui Fan Ivan Bedzhov |
| author_facet | Rui Chen Rui Fan Ivan Bedzhov |
| author_sort | Rui Chen |
| collection | DOAJ |
| description | Summary: Embryonic dormancy (diapause) is a reproductive adaptation that allows some mammalian species to prolong pregnancy and delay birth by temporarily suspending embryonic development just before implantation. Here, we present a step-by-step protocol for inducing and maintaining embryonic diapause in mice by tamoxifen administration or ovariectomy. We describe steps for setting up mouse matings, the administration of pharmacological compounds, the surgical procedure for the removal of the ovaries, postoperative care, and the isolation of dormant embryos. We then describe procedures for triggering exit from diapause by administration of β-estradiol and the subsequent isolation of reactivated embryos.For complete details on the use and execution of this protocol, please refer to Chen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-377f6bd8474e4083946ee90b1d962f35 |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-377f6bd8474e4083946ee90b1d962f352025-08-20T02:57:02ZengElsevierSTAR Protocols2666-16672025-06-016210381310.1016/j.xpro.2025.103813Protocol for induction, maintenance, and exit from embryo dormancy in miceRui Chen0Rui Fan1Ivan Bedzhov2Embryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, Germany; Corresponding authorEmbryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, GermanyEmbryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Röntgenstraße 20, 48149 Münster, Germany; Corresponding authorSummary: Embryonic dormancy (diapause) is a reproductive adaptation that allows some mammalian species to prolong pregnancy and delay birth by temporarily suspending embryonic development just before implantation. Here, we present a step-by-step protocol for inducing and maintaining embryonic diapause in mice by tamoxifen administration or ovariectomy. We describe steps for setting up mouse matings, the administration of pharmacological compounds, the surgical procedure for the removal of the ovaries, postoperative care, and the isolation of dormant embryos. We then describe procedures for triggering exit from diapause by administration of β-estradiol and the subsequent isolation of reactivated embryos.For complete details on the use and execution of this protocol, please refer to Chen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002199Cell BiologyDevelopmental biologyGeneticsMetabolismModel OrganismsStem Cells |
| spellingShingle | Rui Chen Rui Fan Ivan Bedzhov Protocol for induction, maintenance, and exit from embryo dormancy in mice STAR Protocols Cell Biology Developmental biology Genetics Metabolism Model Organisms Stem Cells |
| title | Protocol for induction, maintenance, and exit from embryo dormancy in mice |
| title_full | Protocol for induction, maintenance, and exit from embryo dormancy in mice |
| title_fullStr | Protocol for induction, maintenance, and exit from embryo dormancy in mice |
| title_full_unstemmed | Protocol for induction, maintenance, and exit from embryo dormancy in mice |
| title_short | Protocol for induction, maintenance, and exit from embryo dormancy in mice |
| title_sort | protocol for induction maintenance and exit from embryo dormancy in mice |
| topic | Cell Biology Developmental biology Genetics Metabolism Model Organisms Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002199 |
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