Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing
In order to save manpower and time costs, and to achieve simultaneous detection of multiple animal-derived components in meat and meat products, this study used multiple nucleotide polymorphism (MNP) marker technology based on the principle of high-throughput sequencing, and established a multi-locu...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2025-04-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250178 |
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| author | Yan Yi Zhanyue Jiang Lixia Ma Xiaoni Hou Lun Li Deping Ye Juanlan Du Hai Peng Guoquan Han Huaiping Li Jiangwen Tang Lihua Zhou |
| author_facet | Yan Yi Zhanyue Jiang Lixia Ma Xiaoni Hou Lun Li Deping Ye Juanlan Du Hai Peng Guoquan Han Huaiping Li Jiangwen Tang Lihua Zhou |
| author_sort | Yan Yi |
| collection | DOAJ |
| description | In order to save manpower and time costs, and to achieve simultaneous detection of multiple animal-derived components in meat and meat products, this study used multiple nucleotide polymorphism (MNP) marker technology based on the principle of high-throughput sequencing, and established a multi-locus 10 animal-derived components identification method of cattle, goat, sheep, donkey, horse, chicken, duck, goose, pigeon, quail in meat and meat products. The specific loci of each species could be detected and the species could be accurately identified, including 5 loci for cattle and duck, 3 loci for sheep, 9 loci for chicken and horse, 10 loci for goose and pigeon, 6 loci for quail and 1 locus for donkey and goat, and an adulteration model was established to simulate commercially available samples. The results showed that the method established in this study had high throughput, good repeatability and accuracy, and was able to identify 10 animal-derived components simultaneously with 100% repeatability accuracy. The detection limit was 0.1% (m/m) in simulated samples of chicken, duck and horse. Using the method established in this study to test commercially available samples, 4 samples from 14 commercially available samples were detected to be inconsistent with the labels, of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients. |
| format | Article |
| id | doaj-art-377cd73b4c494b3597a9af7883c59360 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-377cd73b4c494b3597a9af7883c593602025-08-20T03:42:40ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-04-01144925017810.26599/FSHW.2024.9250178Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencingYan Yi0Zhanyue Jiang1Lixia Ma2Xiaoni Hou3Lun Li4Deping Ye5Juanlan Du6Hai Peng7Guoquan Han8Huaiping Li9Jiangwen Tang10Lihua Zhou11National Institute of Measurement and Testing Technology, Chengdu 610000, ChinaNational Institute of Measurement and Testing Technology, Chengdu 610000, ChinaNational Institute of Measurement and Testing Technology, Chengdu 610000, ChinaNational Institute of Measurement and Testing Technology, Chengdu 610000, ChinaInstitute for Systems Biology, Jianghan University, Wuhan 430056, ChinaNational Institute of Measurement and Testing Technology, Chengdu 610000, ChinaMarket Supervision and Administration Bureau of Huaxi District, Guiyang 550000, ChinaInstitute for Systems Biology, Jianghan University, Wuhan 430056, ChinaSichuan Agriculture University, Ya'an 625000, ChinaNational Institute of Measurement and Testing Technology, Chengdu 610000, ChinaNational Institute of Measurement and Testing Technology, Chengdu 610000, ChinaNational Institute of Measurement and Testing Technology, Chengdu 610000, ChinaIn order to save manpower and time costs, and to achieve simultaneous detection of multiple animal-derived components in meat and meat products, this study used multiple nucleotide polymorphism (MNP) marker technology based on the principle of high-throughput sequencing, and established a multi-locus 10 animal-derived components identification method of cattle, goat, sheep, donkey, horse, chicken, duck, goose, pigeon, quail in meat and meat products. The specific loci of each species could be detected and the species could be accurately identified, including 5 loci for cattle and duck, 3 loci for sheep, 9 loci for chicken and horse, 10 loci for goose and pigeon, 6 loci for quail and 1 locus for donkey and goat, and an adulteration model was established to simulate commercially available samples. The results showed that the method established in this study had high throughput, good repeatability and accuracy, and was able to identify 10 animal-derived components simultaneously with 100% repeatability accuracy. The detection limit was 0.1% (m/m) in simulated samples of chicken, duck and horse. Using the method established in this study to test commercially available samples, 4 samples from 14 commercially available samples were detected to be inconsistent with the labels, of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients.https://www.sciopen.com/article/10.26599/FSHW.2024.9250178meat and meat productsmultiple nucleotide polymorphism marker methodhigh-throughput sequencinganimal-derived component identification |
| spellingShingle | Yan Yi Zhanyue Jiang Lixia Ma Xiaoni Hou Lun Li Deping Ye Juanlan Du Hai Peng Guoquan Han Huaiping Li Jiangwen Tang Lihua Zhou Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing Food Science and Human Wellness meat and meat products multiple nucleotide polymorphism marker method high-throughput sequencing animal-derived component identification |
| title | Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing |
| title_full | Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing |
| title_fullStr | Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing |
| title_full_unstemmed | Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing |
| title_short | Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing |
| title_sort | simultaneous identification of multiple animal derived components in meat and meat products by using mnp marker based on high throughput sequencing |
| topic | meat and meat products multiple nucleotide polymorphism marker method high-throughput sequencing animal-derived component identification |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250178 |
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