Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults
Abstract Background Pasteurella multocida is a Gram-negative coccobacillus and is the causative agent of fowl cholera in avian species. P. multocida expresses two large filamentous hemagglutinin (FhaB) proteins encoded by fhaB1 and fhaB2 genes. Previously, it was demonstrated that P. multocida FhaB2...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-025-04668-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849392314481377280 |
|---|---|
| author | Rohana P. Dassanayake Robert E. Briggs Bryan S. Kaplan Harish Menghwar Carly Kanipe Eduardo Casas Fred M. Tatum |
| author_facet | Rohana P. Dassanayake Robert E. Briggs Bryan S. Kaplan Harish Menghwar Carly Kanipe Eduardo Casas Fred M. Tatum |
| author_sort | Rohana P. Dassanayake |
| collection | DOAJ |
| description | Abstract Background Pasteurella multocida is a Gram-negative coccobacillus and is the causative agent of fowl cholera in avian species. P. multocida expresses two large filamentous hemagglutinin (FhaB) proteins encoded by fhaB1 and fhaB2 genes. Previously, it was demonstrated that P. multocida FhaB2 is an important virulence factor in the development of fowl cholera disease. In the current study, we examined the potential role of FhaB1 in fowl cholera disease development. An fhaB1 deletion mutant, devoid of foreign DNA, was constructed using a temperature sensitive plasmid in a well-characterized P. multocida avian strain P-1059 (A:3). Results Real-time PCR assay confirmed the expression of full-length fhaB1 mRNA in the wild-type parent strain and truncated fhaB1 mRNA in the ΔfhaB1 mutant strain. Both parent and the mutant strain produced biofilm; however, the ΔfhaB1 mutant produced significantly lower amounts of biofilm. Turkey poults were challenged intranasally and intramuscularly to assess the virulence of the fhaB1 mutant and the wild-type parent strains. Contrary to our expectation, inactivation of fhaB1 did not reduce virulence by either challenge route. Conclusions These findings indicate that this large and highly conserved FhaB1 protein is not necessary for the development of acute fowl cholera disease in turkeys. |
| format | Article |
| id | doaj-art-37771b72e9e4496cb660bf17f8e773f3 |
| institution | Kabale University |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-37771b72e9e4496cb660bf17f8e773f32025-08-20T03:40:48ZengBMCBMC Veterinary Research1746-61482025-03-012111910.1186/s12917-025-04668-1Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poultsRohana P. Dassanayake0Robert E. Briggs1Bryan S. Kaplan2Harish Menghwar3Carly Kanipe4Eduardo Casas5Fred M. Tatum6Ruminant Diseases and Immunology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Animal Disease CenterRuminant Diseases and Immunology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Animal Disease CenterRuminant Diseases and Immunology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Animal Disease CenterRuminant Diseases and Immunology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Animal Disease CenterInfectious Bacterial Diseases Research Unit, United States Department of Agriculture, Agricultural Research Service, National Animal Disease CenterRuminant Diseases and Immunology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Animal Disease CenterRuminant Diseases and Immunology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Animal Disease CenterAbstract Background Pasteurella multocida is a Gram-negative coccobacillus and is the causative agent of fowl cholera in avian species. P. multocida expresses two large filamentous hemagglutinin (FhaB) proteins encoded by fhaB1 and fhaB2 genes. Previously, it was demonstrated that P. multocida FhaB2 is an important virulence factor in the development of fowl cholera disease. In the current study, we examined the potential role of FhaB1 in fowl cholera disease development. An fhaB1 deletion mutant, devoid of foreign DNA, was constructed using a temperature sensitive plasmid in a well-characterized P. multocida avian strain P-1059 (A:3). Results Real-time PCR assay confirmed the expression of full-length fhaB1 mRNA in the wild-type parent strain and truncated fhaB1 mRNA in the ΔfhaB1 mutant strain. Both parent and the mutant strain produced biofilm; however, the ΔfhaB1 mutant produced significantly lower amounts of biofilm. Turkey poults were challenged intranasally and intramuscularly to assess the virulence of the fhaB1 mutant and the wild-type parent strains. Contrary to our expectation, inactivation of fhaB1 did not reduce virulence by either challenge route. Conclusions These findings indicate that this large and highly conserved FhaB1 protein is not necessary for the development of acute fowl cholera disease in turkeys.https://doi.org/10.1186/s12917-025-04668-1Pasteurella multocidaFowl choleraFilamentous hemagglutininfhaB1Mutants |
| spellingShingle | Rohana P. Dassanayake Robert E. Briggs Bryan S. Kaplan Harish Menghwar Carly Kanipe Eduardo Casas Fred M. Tatum Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults BMC Veterinary Research Pasteurella multocida Fowl cholera Filamentous hemagglutinin fhaB1 Mutants |
| title | Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults |
| title_full | Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults |
| title_fullStr | Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults |
| title_full_unstemmed | Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults |
| title_short | Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults |
| title_sort | pasteurella multocida filamentous hemagglutinin b1 fhab1 gene is not involved with avian fowl cholera pathogenesis in turkey poults |
| topic | Pasteurella multocida Fowl cholera Filamentous hemagglutinin fhaB1 Mutants |
| url | https://doi.org/10.1186/s12917-025-04668-1 |
| work_keys_str_mv | AT rohanapdassanayake pasteurellamultocidafilamentoushemagglutininb1fhab1geneisnotinvolvedwithavianfowlcholerapathogenesisinturkeypoults AT robertebriggs pasteurellamultocidafilamentoushemagglutininb1fhab1geneisnotinvolvedwithavianfowlcholerapathogenesisinturkeypoults AT bryanskaplan pasteurellamultocidafilamentoushemagglutininb1fhab1geneisnotinvolvedwithavianfowlcholerapathogenesisinturkeypoults AT harishmenghwar pasteurellamultocidafilamentoushemagglutininb1fhab1geneisnotinvolvedwithavianfowlcholerapathogenesisinturkeypoults AT carlykanipe pasteurellamultocidafilamentoushemagglutininb1fhab1geneisnotinvolvedwithavianfowlcholerapathogenesisinturkeypoults AT eduardocasas pasteurellamultocidafilamentoushemagglutininb1fhab1geneisnotinvolvedwithavianfowlcholerapathogenesisinturkeypoults AT fredmtatum pasteurellamultocidafilamentoushemagglutininb1fhab1geneisnotinvolvedwithavianfowlcholerapathogenesisinturkeypoults |